##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633396.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	961462
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74210005179612	33.0	33.0	33.0	27.0	33.0
2	31.887611782888975	33.0	33.0	33.0	33.0	33.0
3	31.60824972801837	33.0	33.0	33.0	27.0	33.0
4	31.898142620301165	33.0	33.0	33.0	33.0	33.0
5	31.997812706066387	33.0	33.0	33.0	33.0	33.0
6	35.12876535942138	37.0	37.0	37.0	33.0	37.0
7	35.29812202666356	37.0	37.0	37.0	33.0	37.0
8	35.42781826010804	37.0	37.0	37.0	33.0	37.0
9	35.546226475929366	37.0	37.0	37.0	33.0	37.0
10	35.54463514938708	37.0	37.0	37.0	33.0	37.0
11	35.56392764352621	37.0	37.0	37.0	33.0	37.0
12	35.552622984579735	37.0	37.0	37.0	33.0	37.0
13	35.56904069011568	37.0	37.0	37.0	33.0	37.0
14	35.545599306056815	37.0	37.0	37.0	33.0	37.0
15	35.566943883377604	37.0	37.0	37.0	33.0	37.0
16	35.54991564929243	37.0	37.0	37.0	33.0	37.0
17	35.538875171353624	37.0	37.0	37.0	33.0	37.0
18	35.52503479076656	37.0	37.0	37.0	33.0	37.0
19	35.512166887510894	37.0	37.0	37.0	33.0	37.0
20	35.51450915376791	37.0	37.0	37.0	33.0	37.0
21	35.49123730319035	37.0	37.0	37.0	33.0	37.0
22	35.39391468409568	37.0	37.0	37.0	33.0	37.0
23	35.4467758476154	37.0	37.0	37.0	33.0	37.0
24	35.44453758962913	37.0	37.0	37.0	33.0	37.0
25	35.46487744705459	37.0	37.0	37.0	33.0	37.0
26	35.35163948237164	37.0	37.0	37.0	33.0	37.0
27	35.36486829432676	37.0	37.0	37.0	33.0	37.0
28	35.3800441411101	37.0	37.0	37.0	33.0	37.0
29	35.37927551998935	37.0	37.0	37.0	33.0	37.0
30	35.38062034692999	37.0	37.0	37.0	33.0	37.0
31	35.368163276343736	37.0	37.0	37.0	33.0	37.0
32	35.36087333664773	37.0	37.0	37.0	33.0	37.0
33	35.35056091660409	37.0	37.0	37.0	33.0	37.0
34	35.32622402133418	37.0	37.0	37.0	33.0	37.0
35	35.25749951636154	37.0	37.0	37.0	33.0	37.0
36	35.27708635390686	37.0	37.0	37.0	33.0	37.0
37	35.270968587422075	37.0	37.0	37.0	33.0	37.0
38	35.250698415538004	37.0	37.0	37.0	33.0	37.0
39	35.14178615483503	37.0	37.0	37.0	33.0	37.0
40	34.920474236111254	37.0	37.0	37.0	27.0	37.0
41	35.078045726196144	37.0	37.0	37.0	27.0	37.0
42	35.10433069637698	37.0	37.0	37.0	33.0	37.0
43	34.74986218904127	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	26.0
16	49.0
17	41.0
18	30.0
19	32.0
20	65.0
21	179.0
22	535.0
23	1159.0
24	2376.0
25	4220.0
26	6855.0
27	10585.0
28	14429.0
29	19486.0
30	25263.0
31	32239.0
32	41086.0
33	54368.0
34	76883.0
35	152740.0
36	518809.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.55519198886695	19.93401715304401	14.174975194027429	23.335815664061606
2	15.999176254495758	22.07533943099155	39.3245910914836	22.600893223029097
3	18.08464609105716	27.888881723874682	30.324547408009884	23.701924777058274
4	12.042493619092589	17.937682404504805	40.53150306512374	29.488320911278866
5	13.966438611198361	36.63982559893162	34.68426209252159	14.709473697348413
6	28.328316667741422	40.7781066750428	17.08731078295346	13.806265874262321
7	26.153711743157814	32.82761045158311	22.963674071362156	18.055003733896918
8	24.991315309393404	34.52180117362933	20.607158681258333	19.87972483571894
9	26.83995831348509	15.13309938406302	20.067355756129725	37.959586546322164
10	15.485271388780836	27.76427981553093	33.15866877734117	23.59178001834706
11	33.69951178517716	24.317757748096128	23.793972096661125	18.18875837006559
12	23.812901601935383	26.149239387516094	29.728163983599977	20.309695026948543
13	28.988769186925744	22.756177571240464	25.680266094759858	22.574787147073934
14	22.557625782402216	21.53709662992401	28.742581610089633	27.16269597758414
15	24.288323407477364	28.40965113545829	25.32486983364917	21.97715562341517
16	21.97601153243706	28.613299329562686	25.672673490996008	23.73801564700425
17	22.333175934150283	26.989210181993673	26.318356835735578	24.359257048120465
18	23.837135529017267	25.801435730169263	28.306890963969455	22.054537776844015
19	25.694723244392392	25.523317614216683	28.204338809022094	20.57762033236883
20	25.430022195364977	25.262464871206557	27.962727596098443	21.344785337330023
21	23.401964924250777	26.51108416141252	28.183953187957506	21.902997726379205
22	22.737768107319894	26.570992925357423	28.436589277579355	22.254649689743328
23	22.737352074236945	26.196459142431006	28.77388809958168	22.292300683750373
24	22.682435707287443	26.38221791396852	28.16845595561759	22.766890423126444
25	22.87474699988143	26.69673892467929	28.505962794161395	21.922551281277887
26	23.00309320597174	26.459808083938835	28.299714393288554	22.237384316800874
27	23.00402928040838	26.197187200326173	28.330292824885433	22.468490694380016
28	22.493868712440012	26.32626146431164	28.560151103215723	22.619718720032616
29	23.315014009914066	26.530013666686774	27.836877588505835	22.318094734893318
30	23.317510208411775	26.266664725178945	28.363887496333707	22.051937570075573
31	23.005485396198704	26.258136046978453	28.37886468731994	22.357513869502903
32	21.83050396167503	26.249191335695016	28.684440986747266	23.235863715882687
33	21.852865739883637	26.447223083179573	29.117739442640477	22.58217173429631
34	22.638440208765402	25.871537304646463	28.61506747016523	22.874955016422906
35	22.440616477822317	26.68873028783249	28.21442761128365	22.656225623061545
36	22.95795361647158	26.539062386240953	28.260607283491186	22.24237671379628
37	23.12322275867377	26.14497504841585	28.168247939076114	22.563554253834265
38	23.036999902232225	26.34716712672992	27.85455899453125	22.76127397650661
39	22.774171002078088	26.070713143109142	28.29784224441528	22.8572736103975
40	22.518622680875584	26.64275863216643	28.4623833287223	22.37623535823569
41	22.688156162178018	25.760456471498618	28.425148367798208	23.12623899852516
42	22.058178066319833	26.73938231568174	28.483184982869837	22.719254635128586
43	22.753577364472022	26.18657835671093	28.185929345101524	22.87391493371553
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	77.0
2	102.0
3	372.5
4	643.0
5	643.0
6	835.0
7	1027.0
8	938.5
9	850.0
10	1191.0
11	1532.0
12	1532.0
13	2509.0
14	3486.0
15	6528.0
16	9570.0
17	10801.0
18	12032.0
19	12032.0
20	10841.0
21	9650.0
22	9958.0
23	10266.0
24	12782.0
25	15298.0
26	15298.0
27	18788.0
28	22278.0
29	25959.5
30	29641.0
31	33610.0
32	37579.0
33	37579.0
34	42914.0
35	48249.0
36	51383.5
37	54518.0
38	57023.0
39	59528.0
40	59528.0
41	62266.0
42	65004.0
43	66225.5
44	67447.0
45	68272.0
46	69097.0
47	69097.0
48	69781.0
49	70465.0
50	70766.5
51	71068.0
52	69899.0
53	68730.0
54	68730.0
55	65351.5
56	61973.0
57	55073.0
58	48173.0
59	43835.0
60	39497.0
61	39497.0
62	34521.5
63	29546.0
64	24490.0
65	19434.0
66	16362.5
67	13291.0
68	13291.0
69	11157.5
70	9024.0
71	7425.0
72	5826.0
73	4425.5
74	3025.0
75	3025.0
76	2168.5
77	1312.0
78	1013.0
79	714.0
80	597.5
81	481.0
82	481.0
83	406.0
84	331.0
85	337.0
86	343.0
87	274.5
88	206.0
89	206.0
90	167.0
91	128.0
92	84.5
93	41.0
94	23.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	961462.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.18809420142744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.24513128417945	49.89382786495559
2	7.665954408214697	8.768026456816601
3	1.7375794813144632	2.981065771796369
4	0.7532033288522472	1.7229705169292417
5	0.45920185394604923	1.3130439440468396
6	0.2985210682879013	1.0243110584615553
7	0.22656794735911417	0.9069892381617953
8	0.1781476239638158	0.8150338480802537
9	0.14007262010225097	0.7209437574103458
>10	1.0547724728444174	12.117304682067266
>50	0.14174317493349026	5.720069340374708
>100	0.09246084312125162	9.881907165608935
>500	0.005352024820718412	1.9102065279114306
>1k	9.22762900123864E-4	0.8663951261188428
>5k	3.691051600495456E-4	1.3579047012602061
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7606	0.7910869072308631	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5260	0.5470835040802445	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2974	0.30932059717388727	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1477	0.15362021587956673	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1341	0.13947509105924102	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1249	0.12990633015137362	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1168	0.12148166022162082	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1000	0.10400827073768906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.0400827073768906E-4	0.0	0.0
2	0.0	0.0	1.0400827073768906E-4	0.0	0.0
3	0.0	0.0	3.120248122130672E-4	0.0	0.0
4	0.0	0.0	4.1603308295075624E-4	1.0400827073768906E-4	0.0
5	0.0	0.0	4.1603308295075624E-4	1.0400827073768906E-4	0.0
6	0.0	0.0	0.0011440909781145797	1.0400827073768906E-4	0.0
7	0.0	0.0	0.0028082233099176045	4.1603308295075624E-4	0.0
8	0.0	0.0	0.003224256392868361	4.1603308295075624E-4	0.0
9	0.0	0.0	0.004576363912458319	0.0011440909781145797	0.0
10	3.120248122130672E-4	0.0	0.005408430078359831	0.0011440909781145797	0.0
11	4.1603308295075624E-4	0.0	0.00894471128344126	0.0012480992488522688	0.0
12	4.1603308295075624E-4	0.0	0.010192810532293529	0.0012480992488522688	0.0
13	4.1603308295075624E-4	0.0	0.010712851885981972	0.0012480992488522688	0.0
14	4.1603308295075624E-4	0.0	0.011544918051883487	0.0013521075195899578	0.0
15	6.240496244261344E-4	0.0	0.012480992488522687	0.0014561157903276469	0.0
16	8.320661659015125E-4	0.0	0.013625083466637267	0.001664132331803025	0.0
17	8.320661659015125E-4	0.0	0.014145124820325712	0.001768140602540714	0.0
18	8.320661659015125E-4	0.0	0.0142491330910634	0.001872148873278403	0.0
19	8.320661659015125E-4	0.0	0.01456115790327647	0.001976157144016092	0.0
20	8.320661659015125E-4	0.0	0.014977190986227224	0.001976157144016092	0.0
21	9.360744366392015E-4	0.0	0.01549723233991567	0.0026002067684422264	0.0
22	0.0010400827073768907	0.0	0.01570524888139105	0.003120248122130672	0.0
23	0.0010400827073768907	0.0	0.015809257152128737	0.003744297746556806	0.0
24	0.0011440909781145797	0.0	0.015913265422866425	0.005096405266146764	0.0
25	0.0011440909781145797	0.0	0.016017273693604117	0.005928471432048277	0.0
26	0.0011440909781145797	0.0	0.016017273693604117	0.006864545868687478	0.0
27	0.0011440909781145797	0.0	0.016017273693604117	0.008528678200490502	0.0
28	0.0011440909781145797	0.0	0.016017273693604117	0.017161364671718696	0.0
29	0.0011440909781145797	0.0	0.016017273693604117	0.036090869945978106	0.0
30	0.0012480992488522688	0.0	0.016017273693604117	0.06614926018917024	0.0
31	0.0012480992488522688	0.0	0.016017273693604117	0.13614682639563497	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1405	0.0	24.886122	1
GTATCAA	4010	0.0	23.94389	1
GCCGGCA	230	0.0	20.913044	15
TTCGCCG	225	0.0	20.555555	24
CCGGCAG	250	0.0	19.98	16
ATGCGGA	75	9.263185E-6	19.733334	6
CCTACCG	115	3.0486262E-9	19.304348	24
GCCGCTC	235	0.0	18.893618	27
CGCTCTC	260	0.0	18.500002	29
GCGACAA	110	3.849709E-8	18.5	16
GCGAACT	210	0.0	18.5	28
CCGCTCT	250	0.0	18.5	28
TCGATCA	80	1.6162223E-5	18.5	34
TTCGATC	70	1.2189339E-4	18.5	33
GACTCGG	60	9.235321E-4	18.5	33
CCGCCCA	50	0.007034067	18.499998	9
GCTTTAT	445	0.0	17.876406	1
GCTTCGC	280	0.0	17.839287	22
CTACCGA	115	6.4015694E-8	17.695652	25
AGCTTCG	285	0.0	17.526316	21
>>END_MODULE