FastQCFastQC Report
Fri 10 Feb 2017
ERR1633395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871728
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29270.335769873171448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20610.23642695886790374No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC16790.19260595047996623No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC16380.18790264853256977No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG15030.1724161665106547No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC14630.16782757924490208No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA12970.1487849420920287No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC12730.14603178973257713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12610.14465521355285135No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11330.12997173430244296No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10900.12503900299175888No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10570.12125341849751298No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10100.11586182846025365No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT9950.11414110823559642No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC9630.11047023842299433No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA9630.11047023842299433No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA9510.10909366224326855No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9290.10656993924710459No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9170.10519336306737881No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9140.10484921902244736No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8760.10049006111998238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGGT1051.8189894E-1224.66666635
GGTATCA8500.024.376471
TGCGGTA1103.6379788E-1223.54545436
GAATCGT400.001930814623.1250026
GCGTTAT400.001930814623.12500211
GTATCAA21550.021.7192571
ATCGCTC603.7249556E-521.5833348
CCGGCGT555.1417254E-420.18181823
TATATCG656.899683E-519.9230775
CTTGCGG1403.6379788E-1219.8214334
GGACCGT851.2446017E-619.5882366
ATATCGC701.21876816E-418.56
CGCCGTT609.2343724E-418.525
TTACCGG609.2343724E-418.531
TTCGCCG801.61594E-518.524
TACGTCC609.2343724E-418.54
ACGGTAC1409.458745E-1118.53
CATGCTA4450.017.8764044
CCTTGCG1351.1514203E-917.81481633
AACGATT1258.569259E-917.76000222
Produced by FastQC (version 0.11.5)