##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633395.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	871728
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53092707817117	33.0	33.0	33.0	27.0	33.0
2	31.71898229723033	33.0	33.0	33.0	27.0	33.0
3	31.44759948057192	33.0	33.0	33.0	27.0	33.0
4	31.760768267165904	33.0	33.0	33.0	27.0	33.0
5	31.86568860929097	33.0	33.0	33.0	33.0	33.0
6	34.93531927390195	37.0	37.0	37.0	27.0	37.0
7	35.10859580052493	37.0	37.0	37.0	33.0	37.0
8	35.2358476497256	37.0	37.0	37.0	33.0	37.0
9	35.38111773397206	37.0	37.0	37.0	33.0	37.0
10	35.37096548464659	37.0	37.0	37.0	33.0	37.0
11	35.38397068810455	37.0	37.0	37.0	33.0	37.0
12	35.379054016849295	37.0	37.0	37.0	33.0	37.0
13	35.38225685076079	37.0	37.0	37.0	33.0	37.0
14	35.36964282436723	37.0	37.0	37.0	33.0	37.0
15	35.38528875979664	37.0	37.0	37.0	33.0	37.0
16	35.3688799717343	37.0	37.0	37.0	33.0	37.0
17	35.35040402510875	37.0	37.0	37.0	33.0	37.0
18	35.3387604849219	37.0	37.0	37.0	33.0	37.0
19	35.3232189398528	37.0	37.0	37.0	33.0	37.0
20	35.32353096378687	37.0	37.0	37.0	33.0	37.0
21	35.328102343850375	37.0	37.0	37.0	33.0	37.0
22	35.21610066442744	37.0	37.0	37.0	33.0	37.0
23	35.26776471559936	37.0	37.0	37.0	33.0	37.0
24	35.26742630728851	37.0	37.0	37.0	33.0	37.0
25	35.277924994952556	37.0	37.0	37.0	33.0	37.0
26	35.17025035332122	37.0	37.0	37.0	33.0	37.0
27	35.17965810436283	37.0	37.0	37.0	33.0	37.0
28	35.194210808876164	37.0	37.0	37.0	33.0	37.0
29	35.20125543747591	37.0	37.0	37.0	33.0	37.0
30	35.19503904887763	37.0	37.0	37.0	33.0	37.0
31	35.18751147146816	37.0	37.0	37.0	33.0	37.0
32	35.179830176385295	37.0	37.0	37.0	33.0	37.0
33	35.18165413982343	37.0	37.0	37.0	33.0	37.0
34	35.1655803186315	37.0	37.0	37.0	33.0	37.0
35	35.102199309876475	37.0	37.0	37.0	33.0	37.0
36	35.12831181285906	37.0	37.0	37.0	33.0	37.0
37	35.117419653836976	37.0	37.0	37.0	33.0	37.0
38	35.11444969072922	37.0	37.0	37.0	33.0	37.0
39	35.0097908980783	37.0	37.0	37.0	27.0	37.0
40	34.80235233926179	37.0	37.0	37.0	27.0	37.0
41	34.99635666171099	37.0	37.0	37.0	27.0	37.0
42	35.03756102821063	37.0	37.0	37.0	27.0	37.0
43	34.67571077216746	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	21.0
16	45.0
17	33.0
18	36.0
19	38.0
20	92.0
21	217.0
22	576.0
23	1377.0
24	2649.0
25	4590.0
26	7360.0
27	10775.0
28	14755.0
29	19654.0
30	25292.0
31	31681.0
32	40180.0
33	52764.0
34	75884.0
35	147876.0
36	435830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58946368592038	20.093194207367436	13.330649009782869	24.986693096929315
2	16.806159719545548	20.999096048308648	38.543674173595434	23.651070058550374
3	18.103123910210524	27.064978984270322	29.426036561863334	25.405860543655816
4	12.729199933924344	17.801309582805647	39.10680854578492	30.362681937485085
5	14.23873042967531	36.9436337940275	34.89081456601142	13.926821210285778
6	29.948332507387626	39.465521355285134	17.013563863957565	13.572582273369674
7	27.432754253620395	31.762889341629496	22.958766954829947	17.84558944992016
8	24.874387423600023	35.37812253363434	20.02906870032854	19.71842134243709
9	26.476722096800838	15.15839803241378	19.80583392984968	38.559045940935704
10	16.082310078373073	27.17074592074592	33.001807903382705	23.7451360974983
11	34.27537029899235	23.53876438522108	23.499531964098892	18.686333351687683
12	24.105684341904816	25.50382688177964	28.92725712607602	21.463231650239525
13	28.008507240790703	22.003193656736965	25.546730172714426	24.441568929757906
14	22.507938255969755	20.685810252739387	27.158471449810033	29.647780041480832
15	24.850182625773176	27.64279683571022	24.49858212653488	23.008438411981718
16	23.398238900207406	27.62627792155351	24.627865572747464	24.34761760549162
17	22.910930932584474	26.98444927775636	25.428918194666227	24.675701594992933
18	23.458578822752052	26.558169520767944	27.27077712313933	22.712474533340675
19	25.914161298019568	25.43385092597691	26.479016390433713	22.172971385569813
20	24.9471165317622	25.584471302975242	26.955541177982123	22.512870987280436
21	24.12185911201659	26.420282473432078	26.6721959143219	22.785662500229428
22	23.383555420956995	26.473624800396458	26.51939595837234	23.623423820274216
23	23.44504249031808	26.42957436264523	27.184970541269752	22.940412605766937
24	23.897247765358003	26.956688324798563	25.316268377292	23.829795532551437
25	23.80972046326377	26.468577354404125	26.812491969972285	22.909210212359817
26	23.89151203127581	26.690435548703267	26.867096158434737	22.550956261586183
27	23.937053759888403	26.437489675678655	25.832828588734102	23.792627975698842
28	22.96312611273241	26.139346218086374	27.350962685608355	23.546564983572857
29	24.974189196630142	25.974845364609145	26.103325808050215	22.947639630710498
30	24.231985206394654	25.672113319751116	27.11556815887525	22.980333314978985
31	23.788154103114735	25.96899491584531	26.805035515665438	23.43781546537452
32	22.46526439439825	25.829157718921497	27.211125488684544	24.494452397995705
33	22.877319530862838	26.11445313216967	27.53393260283024	23.474294734137256
34	23.617688086192025	26.09173962520419	27.16592790411688	23.124644384486903
35	23.85514747719472	26.603940678743825	26.427280069012355	23.113631775049097
36	24.618458968852668	26.333328744746066	25.930336068131343	23.11787621826992
37	23.666327111209	25.80598535322945	26.35707468384634	24.170612851715216
38	24.14434318961878	26.02405796303434	26.27597140392416	23.55562744342272
39	23.134280417744986	26.245342583925265	26.787943028100507	23.832433970229246
40	23.409136794963565	26.77050639649065	27.011292513261015	22.809064295284767
41	23.36095662867316	25.387391479911166	27.123598186590314	24.12805370482536
42	22.787268505772442	26.832567039259953	26.786337022557493	23.59382743241011
43	23.95586696767799	25.18377291999339	26.761099792595854	24.09926031973276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	27.5
2	31.0
3	136.5
4	242.0
5	242.0
6	316.0
7	390.0
8	380.0
9	370.0
10	508.0
11	646.0
12	646.0
13	1017.5
14	1389.0
15	2617.5
16	3846.0
17	4305.0
18	4764.0
19	4764.0
20	4430.0
21	4096.0
22	4344.5
23	4593.0
24	6260.5
25	7928.0
26	7928.0
27	10682.5
28	13437.0
29	16380.5
30	19324.0
31	24052.5
32	28781.0
33	28781.0
34	36058.0
35	43335.0
36	47763.0
37	52191.0
38	56582.0
39	60973.0
40	60973.0
41	64377.5
42	67782.0
43	70182.5
44	72583.0
45	70694.5
46	68806.0
47	68806.0
48	66618.5
49	64431.0
50	64972.0
51	65513.0
52	63588.5
53	61664.0
54	61664.0
55	60965.0
56	60266.0
57	52014.0
58	43762.0
59	39419.0
60	35076.0
61	35076.0
62	31948.5
63	28821.0
64	23044.0
65	17267.0
66	14326.0
67	11385.0
68	11385.0
69	9767.0
70	8149.0
71	7066.5
72	5984.0
73	6037.5
74	6091.0
75	6091.0
76	5206.5
77	4322.0
78	3161.5
79	2001.0
80	1292.0
81	583.0
82	583.0
83	485.5
84	388.0
85	338.0
86	288.0
87	213.5
88	139.0
89	139.0
90	95.5
91	52.0
92	33.0
93	14.0
94	7.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	871728.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.72127056793734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.8609553212775	41.832548354295405
2	7.871678461799091	7.670363523222365
3	2.054114259321272	3.0023716981754967
4	0.9365067650910035	1.8251119796281006
5	0.5464216889557721	1.3311179475901735
6	0.3832171389781415	1.120249554865493
7	0.27467616103093123	0.9367800092105227
8	0.21813551104557038	0.8502271403301214
9	0.17155710163516308	0.7522631969956114
>10	1.3152190558730537	13.106477548567222
>50	0.19883349260033994	6.7941304780799365
>100	0.15408959900809943	14.66002726780489
>500	0.011484939056354318	3.8577055754221554
>1k	0.0031105043277626283	2.260625725812475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2927	0.335769873171448	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2061	0.23642695886790374	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1679	0.19260595047996623	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1638	0.18790264853256977	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1503	0.1724161665106547	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1463	0.16782757924490208	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1297	0.1487849420920287	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1273	0.14603178973257713	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1261	0.14465521355285135	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1133	0.12997173430244296	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1090	0.12503900299175888	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1057	0.12125341849751298	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1010	0.11586182846025365	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	995	0.11414110823559642	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	963	0.11047023842299433	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	963	0.11047023842299433	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	951	0.10909366224326855	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	929	0.10656993924710459	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	917	0.10519336306737881	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	914	0.10484921902244736	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	876	0.10049006111998238	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.147146816438155E-4	0.0	0.0
2	0.0	0.0	1.147146816438155E-4	0.0	0.0
3	0.0	0.0	2.29429363287631E-4	0.0	0.0
4	0.0	0.0	2.29429363287631E-4	0.0	0.0
5	0.0	0.0	5.735734082190775E-4	0.0	0.0
6	0.0	0.0	0.001376576179725786	0.0	0.0
7	0.0	0.0	0.002982581722739203	1.147146816438155E-4	0.0
8	0.0	0.0	0.003212011086026834	1.147146816438155E-4	0.0
9	0.0	0.0	0.003670869812602096	8.030027715067085E-4	0.0
10	0.0	0.0	0.004129728539177358	8.030027715067085E-4	0.0
11	0.0	0.0	0.005735734082190775	8.030027715067085E-4	0.0
12	1.147146816438155E-4	0.0	0.006424022172053668	8.030027715067085E-4	0.0
13	1.147146816438155E-4	0.0	0.006997595580272745	9.17717453150524E-4	0.0
14	1.147146816438155E-4	0.0	0.0071123102619165615	0.0010324321347943395	0.0
15	1.147146816438155E-4	0.0	0.007227024943560377	0.0010324321347943395	0.0
16	1.147146816438155E-4	0.0	0.007571168988491823	0.0010324321347943395	0.0
17	1.147146816438155E-4	0.0	0.00791531303342327	0.0010324321347943395	0.0
18	1.147146816438155E-4	0.0	0.0081447423967109	0.0010324321347943395	0.0
19	1.147146816438155E-4	0.0	0.00837417175999853	0.0010324321347943395	0.0
20	1.147146816438155E-4	0.0	0.00837417175999853	0.0010324321347943395	0.0
21	1.147146816438155E-4	0.0	0.008833030486573794	0.001147146816438155	0.0
22	1.147146816438155E-4	0.0	0.008833030486573794	0.001606005543013417	0.0
23	1.147146816438155E-4	0.0	0.00894774516821761	0.0017207202246572325	0.0
24	1.147146816438155E-4	0.0	0.009062459849861424	0.0024090083145201255	0.0
25	1.147146816438155E-4	0.0	0.009062459849861424	0.002523722996163941	0.0
26	1.147146816438155E-4	0.0	0.009062459849861424	0.003212011086026834	0.0
27	1.147146816438155E-4	0.0	0.009062459849861424	0.0042444432208211735	0.0
28	1.147146816438155E-4	0.0	0.00917717453150524	0.011700897527669181	0.0
29	1.147146816438155E-4	0.0	0.00917717453150524	0.025810803369858488	0.0
30	1.147146816438155E-4	0.0	0.00917717453150524	0.04519758456766331	0.0
31	1.147146816438155E-4	0.0	0.00917717453150524	0.10817594479011802	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGGT	105	1.8189894E-12	24.666666	35
GGTATCA	850	0.0	24.37647	1
TGCGGTA	110	3.6379788E-12	23.545454	36
GAATCGT	40	0.0019308146	23.125002	6
GCGTTAT	40	0.0019308146	23.125002	11
GTATCAA	2155	0.0	21.719257	1
ATCGCTC	60	3.7249556E-5	21.583334	8
CCGGCGT	55	5.1417254E-4	20.181818	23
TATATCG	65	6.899683E-5	19.923077	5
CTTGCGG	140	3.6379788E-12	19.82143	34
GGACCGT	85	1.2446017E-6	19.588236	6
ATATCGC	70	1.21876816E-4	18.5	6
CGCCGTT	60	9.2343724E-4	18.5	25
TTACCGG	60	9.2343724E-4	18.5	31
TTCGCCG	80	1.61594E-5	18.5	24
TACGTCC	60	9.2343724E-4	18.5	4
ACGGTAC	140	9.458745E-11	18.5	3
CATGCTA	445	0.0	17.876404	4
CCTTGCG	135	1.1514203E-9	17.814816	33
AACGATT	125	8.569259E-9	17.760002	22
>>END_MODULE