FastQCFastQC Report
Fri 10 Feb 2017
ERR1633394.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633394.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006305
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74560.7409284461470429No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55060.54715021787629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28570.2839099477792518No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA17420.17310855058853927No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15180.15084889770000148No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14040.13952032435494208No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12810.12729738995632536No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG12000.11924814047430947No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11990.11914876702391422No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11260.11189450514506039No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11070.11000640958755051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.028.8273071
CGTTATT454.0076065E-628.77777710
CGCCGTT754.620233E-1027.13333325
TTACCGG509.089619E-625.90000231
TAGGACC1300.024.1923084
GACCGTT701.9224353E-723.7857137
GTATCAA35350.023.6025451
TTTACCG603.7256945E-521.58333230
CGGCGTA450.003825482420.55555512
ATAGCGG450.003825482420.5555557
TCGGCGT450.003825482420.55555511
TTCGCCG2850.020.12280824
TGTTACG656.9010406E-519.92307716
GCCGGCA3100.019.69354815
GTTACGG851.2449927E-619.58823417
CCGTTTA851.2449927E-619.58823427
TCCGCCG2750.019.50909231
CCGCTCT2950.019.44067828
CGCCGTC3100.019.09677526
CCGTCCG3150.018.7936528
Produced by FastQC (version 0.11.5)