FastQCFastQC Report
Fri 10 Feb 2017
ERR1633393.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633393.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009832
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28230.2795514501422019No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18600.18418905322865584No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC13540.13408170864064517No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG11570.11457351321804021No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11560.11447448684533666No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10880.10774069350149333No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10640.10536406055660742No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10580.10476990232038597No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10350.10249229574820365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8850.021.3220331
GTATCAA21050.021.1805231
CGGCGTA450.003825491820.55555512
TCGGCGT450.003825491820.55555511
GGTCGTG555.142499E-420.18181811
TTAACGG2900.019.1379335
CCGGTAT500.00703430418.51
AACGGCC3200.017.92187537
TAACGGC3350.016.56716336
TATTAGA4100.016.2439022
GTCGCCC2850.016.2280737
ATTAGAG3650.016.219183
GTATTAG4800.016.18751
GTGACAC3850.015.37662324
TTTACAC1701.4842954E-915.2352943
GCCCTAA855.363349E-415.2352941
GCTCGGA2950.015.05084711
TTTAACG3700.015.034
TATACTG1853.0377123E-1015.05
TCAACGC29800.014.8372484
Produced by FastQC (version 0.11.5)