##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633393.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1009832
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.782169707436484	33.0	33.0	33.0	27.0	33.0
2	31.906079427073017	33.0	33.0	33.0	33.0	33.0
3	31.63016521560022	33.0	33.0	33.0	27.0	33.0
4	31.927885034342346	33.0	33.0	33.0	33.0	33.0
5	32.019760712673	33.0	33.0	33.0	33.0	33.0
6	35.17306244999168	37.0	37.0	37.0	33.0	37.0
7	35.333308906828066	37.0	37.0	37.0	33.0	37.0
8	35.45859905410009	37.0	37.0	37.0	33.0	37.0
9	35.58603411260487	37.0	37.0	37.0	33.0	37.0
10	35.589556480681935	37.0	37.0	37.0	33.0	37.0
11	35.61783346140744	37.0	37.0	37.0	33.0	37.0
12	35.60181792615009	37.0	37.0	37.0	33.0	37.0
13	35.60630481109729	37.0	37.0	37.0	33.0	37.0
14	35.58983375452551	37.0	37.0	37.0	33.0	37.0
15	35.608258601430734	37.0	37.0	37.0	33.0	37.0
16	35.58777994755563	37.0	37.0	37.0	33.0	37.0
17	35.575481862329575	37.0	37.0	37.0	33.0	37.0
18	35.55582413708419	37.0	37.0	37.0	33.0	37.0
19	35.55587266000681	37.0	37.0	37.0	33.0	37.0
20	35.55362278081899	37.0	37.0	37.0	33.0	37.0
21	35.55321479216345	37.0	37.0	37.0	33.0	37.0
22	35.46147775075458	37.0	37.0	37.0	33.0	37.0
23	35.51369237655372	37.0	37.0	37.0	33.0	37.0
24	35.49372667928923	37.0	37.0	37.0	33.0	37.0
25	35.52160062267783	37.0	37.0	37.0	33.0	37.0
26	35.40694887862536	37.0	37.0	37.0	33.0	37.0
27	35.41625636739577	37.0	37.0	37.0	33.0	37.0
28	35.44259738253491	37.0	37.0	37.0	33.0	37.0
29	35.44965400185377	37.0	37.0	37.0	33.0	37.0
30	35.45901298433799	37.0	37.0	37.0	33.0	37.0
31	35.45573224061032	37.0	37.0	37.0	33.0	37.0
32	35.444536318912455	37.0	37.0	37.0	33.0	37.0
33	35.44858946834721	37.0	37.0	37.0	33.0	37.0
34	35.423708101941706	37.0	37.0	37.0	33.0	37.0
35	35.367019464623816	37.0	37.0	37.0	33.0	37.0
36	35.397143287200244	37.0	37.0	37.0	33.0	37.0
37	35.399514968826495	37.0	37.0	37.0	33.0	37.0
38	35.38954598388643	37.0	37.0	37.0	33.0	37.0
39	35.29295962100627	37.0	37.0	37.0	33.0	37.0
40	35.0910715841843	37.0	37.0	37.0	33.0	37.0
41	35.26946561408234	37.0	37.0	37.0	33.0	37.0
42	35.32041864389324	37.0	37.0	37.0	33.0	37.0
43	34.98988544629206	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	32.0
16	48.0
17	35.0
18	30.0
19	30.0
20	55.0
21	171.0
22	472.0
23	1080.0
24	2292.0
25	4082.0
26	6854.0
27	9933.0
28	14273.0
29	19341.0
30	25289.0
31	32323.0
32	41488.0
33	54255.0
34	78473.0
35	159478.0
36	559795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.458975354316365	20.068189560243685	13.504622551077802	24.968212534362152
2	16.275380459323927	21.108758684612887	38.62087951263181	23.99498134343138
3	17.959819059011796	26.67057490750937	29.686522114569552	25.683083918909283
4	12.608037772619603	17.810586315347503	39.04580167790286	30.535574234130035
5	14.077391090795299	36.761065206885895	35.28131411957632	13.880229582742476
6	30.140855112533572	39.31941154568285	17.198009173803168	13.341724167980418
7	27.373662153704775	31.676655126793367	23.11325052087872	17.836432198623136
8	24.79026214261382	35.61493396921468	20.222670701661265	19.372133186510233
9	26.12216685547695	15.501291303900055	20.008674710248833	38.36786713037416
10	15.336115314230486	27.62400082389942	33.451009672896085	23.588874188974007
11	34.3783916532651	23.27902066878451	23.87149545667002	18.471092221280372
12	23.50064169089512	25.408978919265778	29.77475461264844	21.31562477719066
13	28.17102250671399	21.989598269811218	25.630104809512872	24.209274413961925
14	22.15655673418945	20.772366096538832	27.321376228917288	29.749700940354433
15	24.329096324933257	28.239251677506754	24.029145442014116	23.402506555545873
16	23.420628381750628	28.06763897361145	24.686086398529657	23.825646246108263
17	22.859643980384856	27.26057403607729	26.041559388096235	23.838222595441618
18	23.441027814527565	27.033011431604464	27.170658089662442	22.35530266420553
19	25.313616522352234	26.11553208850581	26.63908452098963	21.931766868152327
20	24.580128179736825	25.99313549184419	26.94933414666994	22.477402181749042
21	23.577189076994987	26.678001885462137	26.974288792591246	22.770520244951637
22	23.227625981351355	26.523817823162666	26.813271910575224	23.435284284910757
23	23.225645453897283	26.33527160953505	27.275229939237416	23.16385299733025
24	23.55094708822854	27.10500360455997	25.76715730933462	23.576891997876874
25	23.598578773498957	26.638094257262594	26.780890286701155	22.982436682537294
26	23.598281694380848	26.805052721640827	26.86466659800838	22.73199898596994
27	23.395079577593105	26.784059130627668	26.295463007708214	23.525398284071013
28	22.967186621140943	26.547287073493415	27.127086485672862	23.35843981969278
29	24.424458721846804	26.33140958099961	26.220599069944306	23.023532627209278
30	23.82970632738911	26.077406935014935	26.9731995024915	23.119687235104454
31	23.71374644495322	26.237928685167432	26.75098432214467	23.297340547734674
32	22.679118902946232	26.086913466794474	27.21274429806146	24.02122333219783
33	22.672385109602388	26.285263291319744	27.590628936298316	23.451722662779552
34	23.536192158695705	26.024427825618517	27.09282336071743	23.34655665496835
35	23.46588343407616	26.852090248675026	26.569766060097127	23.112260257151686
36	24.3523675225186	26.372109420180784	26.354482725839546	22.92104033146107
37	23.61887917990319	26.000760522542365	26.49272354213374	23.887636755420704
38	23.64729974886912	26.556991658018365	26.36101846643798	23.434690126674536
39	23.29288436096301	26.24694008508346	26.80237900957783	23.6577965443757
40	23.332791989162555	26.975774188181795	26.95785041472245	22.7335834079332
41	23.368837588826658	25.85212193711429	27.004788915383944	23.774251558675104
42	22.884994731796972	26.839810978459784	26.919230129368053	23.35596416037519
43	23.71820263172488	25.722397388872604	26.795150084370466	23.764249895032044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	28.5
2	47.0
3	152.5
4	258.0
5	258.0
6	314.0
7	370.0
8	349.0
9	328.0
10	479.0
11	630.0
12	630.0
13	942.5
14	1255.0
15	2410.0
16	3565.0
17	4241.5
18	4918.0
19	4918.0
20	4733.5
21	4549.0
22	5295.5
23	6042.0
24	8096.0
25	10150.0
26	10150.0
27	13705.5
28	17261.0
29	21173.0
30	25085.0
31	30523.5
32	35962.0
33	35962.0
34	43745.5
35	51529.0
36	56741.0
37	61953.0
38	66795.0
39	71637.0
40	71637.0
41	75374.5
42	79112.0
43	81355.0
44	83598.0
45	81842.5
46	80087.0
47	80087.0
48	77962.0
49	75837.0
50	75662.0
51	75487.0
52	72835.0
53	70183.0
54	70183.0
55	67662.0
56	65141.0
57	57046.0
58	48951.0
59	45164.5
60	41378.0
61	41378.0
62	36524.5
63	31671.0
64	26798.0
65	21925.0
66	18490.5
67	15056.0
68	15056.0
69	12549.5
70	10043.0
71	8230.0
72	6417.0
73	5180.5
74	3944.0
75	3944.0
76	3078.0
77	2212.0
78	1729.0
79	1246.0
80	1002.5
81	759.0
82	759.0
83	629.0
84	499.0
85	448.5
86	398.0
87	311.5
88	225.0
89	225.0
90	158.5
91	92.0
92	53.5
93	15.0
94	10.5
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1009832.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.77217648407227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.86481772959938	49.02825227163041
2	8.830119944043012	10.202704955655584
3	2.3873218052082232	4.137623299642904
4	1.0920515763444538	2.523607855931244
5	0.616218733149353	1.7800148702147924
6	0.391848565811036	1.3582766699439464
7	0.2716886624093243	1.098723174740311
8	0.19583811592559613	0.9051195356449394
9	0.15588462261233582	0.8105214535841581
>10	0.9928944477489878	11.107470395931418
>50	0.10975239614047694	4.398923401062034
>100	0.08394754179773858	9.070986022220563
>500	0.006058069826253433	2.3183197115236895
>1k	0.00155778938389374	1.2594563822740195
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2823	0.2795514501422019	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1860	0.18418905322865584	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1354	0.13408170864064517	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1157	0.11457351321804021	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1156	0.11447448684533666	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1088	0.10774069350149333	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1064	0.10536406055660742	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1058	0.10476990232038597	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1035	0.10249229574820365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	9.902637270357842E-5	0.0	0.0
3	0.0	0.0	9.902637270357842E-5	0.0	0.0
4	0.0	0.0	9.902637270357842E-5	0.0	0.0
5	0.0	0.0	1.9805274540715684E-4	0.0	0.0
6	0.0	0.0	3.961054908143137E-4	0.0	0.0
7	0.0	0.0	0.0014853955905536763	0.0	0.0
8	0.0	0.0	0.0017824747086644115	0.0	0.0
9	0.0	0.0	0.0020795538267751467	3.961054908143137E-4	0.0
10	2.9707911811073523E-4	0.0	0.002178580199478725	3.961054908143137E-4	0.0
11	2.9707911811073523E-4	0.0	0.0032678702992180878	4.951318635178921E-4	0.0
12	2.9707911811073523E-4	0.0	0.0037630021627359797	4.951318635178921E-4	0.0
13	2.9707911811073523E-4	0.0	0.0039610549081431365	4.951318635178921E-4	0.0
14	2.9707911811073523E-4	0.0	0.0044561867716610285	5.941582362214705E-4	0.0
15	2.9707911811073523E-4	0.0	0.004753265889771764	5.941582362214705E-4	0.0
16	2.9707911811073523E-4	0.0	0.0052483977532896565	5.941582362214705E-4	1.9805274540715684E-4
17	2.9707911811073523E-4	0.0	0.005347424125993235	6.93184608925049E-4	1.9805274540715684E-4
18	2.9707911811073523E-4	0.0	0.005347424125993235	7.922109816286273E-4	1.9805274540715684E-4
19	2.9707911811073523E-4	0.0	0.005545476871400392	7.922109816286273E-4	1.9805274540715684E-4
20	2.9707911811073523E-4	0.0	0.0057435296168075484	8.912373543322057E-4	1.9805274540715684E-4
21	2.9707911811073523E-4	0.0	0.005941582362214705	9.902637270357841E-4	1.9805274540715684E-4
22	2.9707911811073523E-4	0.0	0.006040608734918284	0.0010892900997393625	1.9805274540715684E-4
23	2.9707911811073523E-4	0.0	0.006139635107621862	0.0014853955905536763	1.9805274540715684E-4
24	2.9707911811073523E-4	0.0	0.006139635107621862	0.001683448335960833	1.9805274540715684E-4
25	2.9707911811073523E-4	0.0	0.006139635107621862	0.0018815010813679899	1.9805274540715684E-4
26	2.9707911811073523E-4	0.0	0.006139635107621862	0.002376632944885882	1.9805274540715684E-4
27	2.9707911811073523E-4	0.0	0.006139635107621862	0.004258134026253872	1.9805274540715684E-4
28	2.9707911811073523E-4	0.0	0.006139635107621862	0.01000166364306142	1.9805274540715684E-4
29	2.9707911811073523E-4	0.0	0.006139635107621862	0.0238653558215624	1.9805274540715684E-4
30	2.9707911811073523E-4	0.0	0.006139635107621862	0.04337355124416735	1.9805274540715684E-4
31	2.9707911811073523E-4	0.0	0.006139635107621862	0.09754097711302474	1.9805274540715684E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	885	0.0	21.322033	1
GTATCAA	2105	0.0	21.180523	1
CGGCGTA	45	0.0038254918	20.555555	12
TCGGCGT	45	0.0038254918	20.555555	11
GGTCGTG	55	5.142499E-4	20.181818	11
TTAACGG	290	0.0	19.13793	35
CCGGTAT	50	0.007034304	18.5	1
AACGGCC	320	0.0	17.921875	37
TAACGGC	335	0.0	16.567163	36
TATTAGA	410	0.0	16.243902	2
GTCGCCC	285	0.0	16.22807	37
ATTAGAG	365	0.0	16.21918	3
GTATTAG	480	0.0	16.1875	1
GTGACAC	385	0.0	15.376623	24
TTTACAC	170	1.4842954E-9	15.235294	3
GCCCTAA	85	5.363349E-4	15.235294	1
GCTCGGA	295	0.0	15.050847	11
TTTAACG	370	0.0	15.0	34
TATACTG	185	3.0377123E-10	15.0	5
TCAACGC	2980	0.0	14.837248	4
>>END_MODULE