FastQCFastQC Report
Fri 10 Feb 2017
ERR1633392.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633392.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877165
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48430.5521196126156425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35110.4002667685099155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19620.22367513523681407No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12430.14170652043800197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9750.023.5282041
GTATCAA26950.023.1335811
TCGCCAT1251.8189894E-1120.7213
AGCGACG450.003825096220.55555529
GTCGCCA1254.110916E-1019.2412
TGCTCGC1850.019.00000210
GTACTAG609.234435E-418.51
CCGTTAA701.2187798E-418.516
CGGCAGC1700.018.49999817
GCCGGCA1700.018.49999815
CTGCTCG3250.017.6461549
CGCCGTC1900.017.52631626
CCGGCAG1800.017.47222116
GAGTCGT752.0665627E-417.26666619
CGTCCGC1950.017.07692329
GCCGTCC1950.017.07692327
TTAGGCA1201.0403346E-716.9583344
GCTTCGC1757.2759576E-1216.91428622
TTCGCCG1655.4569682E-1116.81818224
CCGTCCG2000.016.65000228
Produced by FastQC (version 0.11.5)