##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633392.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	877165
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46815137402883	33.0	33.0	33.0	27.0	33.0
2	31.681902492689517	33.0	33.0	33.0	27.0	33.0
3	31.410247786904403	33.0	33.0	33.0	27.0	33.0
4	31.714536033699474	33.0	33.0	33.0	27.0	33.0
5	31.82510702091397	33.0	33.0	33.0	33.0	33.0
6	34.84676315174454	37.0	37.0	37.0	27.0	37.0
7	35.01492421608249	37.0	37.0	37.0	33.0	37.0
8	35.15500162455182	37.0	37.0	37.0	33.0	37.0
9	35.29207503719368	37.0	37.0	37.0	33.0	37.0
10	35.307484908768586	37.0	37.0	37.0	33.0	37.0
11	35.306815707421066	37.0	37.0	37.0	33.0	37.0
12	35.32391055274663	37.0	37.0	37.0	33.0	37.0
13	35.31545376297504	37.0	37.0	37.0	33.0	37.0
14	35.30803668637029	37.0	37.0	37.0	33.0	37.0
15	35.31439352915358	37.0	37.0	37.0	33.0	37.0
16	35.285105994881235	37.0	37.0	37.0	33.0	37.0
17	35.29050634715247	37.0	37.0	37.0	33.0	37.0
18	35.267432011081155	37.0	37.0	37.0	33.0	37.0
19	35.2725576145879	37.0	37.0	37.0	33.0	37.0
20	35.26598416489486	37.0	37.0	37.0	33.0	37.0
21	35.26244207190209	37.0	37.0	37.0	33.0	37.0
22	35.15263604908996	37.0	37.0	37.0	33.0	37.0
23	35.2038464827028	37.0	37.0	37.0	33.0	37.0
24	35.19469085063813	37.0	37.0	37.0	33.0	37.0
25	35.21196696174608	37.0	37.0	37.0	33.0	37.0
26	35.087207081905916	37.0	37.0	37.0	33.0	37.0
27	35.11146249565361	37.0	37.0	37.0	33.0	37.0
28	35.11424076428038	37.0	37.0	37.0	33.0	37.0
29	35.119441610187366	37.0	37.0	37.0	33.0	37.0
30	35.12957539345505	37.0	37.0	37.0	33.0	37.0
31	35.12296888270736	37.0	37.0	37.0	33.0	37.0
32	35.11618566632276	37.0	37.0	37.0	33.0	37.0
33	35.10204009507903	37.0	37.0	37.0	33.0	37.0
34	35.07838434046046	37.0	37.0	37.0	33.0	37.0
35	35.01493561644617	37.0	37.0	37.0	27.0	37.0
36	35.040562493943554	37.0	37.0	37.0	27.0	37.0
37	35.029620424891554	37.0	37.0	37.0	27.0	37.0
38	35.0174060752538	37.0	37.0	37.0	27.0	37.0
39	34.911700763254345	37.0	37.0	37.0	27.0	37.0
40	34.68607958593879	37.0	37.0	37.0	27.0	37.0
41	34.863701812087804	37.0	37.0	37.0	27.0	37.0
42	34.90700609349438	37.0	37.0	37.0	27.0	37.0
43	34.511090843797916	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	30.0
16	38.0
17	45.0
18	42.0
19	40.0
20	117.0
21	268.0
22	684.0
23	1471.0
24	2939.0
25	4978.0
26	7884.0
27	11524.0
28	15539.0
29	20726.0
30	26495.0
31	33301.0
32	41918.0
33	54929.0
34	78231.0
35	152092.0
36	423870.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.40942696072005	20.730079289529336	14.028831519725479	23.831662230025138
2	15.864404074489977	22.649900531826965	39.107807539060495	22.377887854622564
3	18.08884303409279	27.851202453358265	30.766959466006966	23.292995046541982
4	12.579731293428258	18.372598085878938	39.74964801377164	29.29802260692116
5	13.776883482583093	37.35021347180975	35.09134541391871	13.781557631688452
6	28.264693643727234	40.76701646782532	17.40288315197255	13.565406736474895
7	25.486995035141618	33.20971538992094	23.51917826178655	17.784111313150888
8	24.295885038732738	35.81777658707313	20.78172293696169	19.104615437232447
9	26.211602150108586	15.535845593474432	20.26175234989996	37.99079990651702
10	14.732575969173418	28.501935211733255	33.906847628439344	22.85864119065398
11	33.219747709951946	24.3718114607856	24.124765580022004	18.28367524924045
12	22.741331448473208	26.502425427371136	30.476250192381137	20.279992931774522
13	28.441399280637054	22.71978476113388	26.496725245535334	22.342090712693736
14	21.96781677335507	21.852787103908614	28.55551692098978	27.623879201746536
15	23.916936950288715	28.831975740026106	25.338790307410807	21.91229700227437
16	22.16367502123318	29.066139209840795	25.669400853887236	23.100784915038787
17	22.11704753381633	27.71040796201399	26.602748627681223	23.56979587648846
18	23.076388136781564	26.734194820814782	28.234710687270926	21.95470635513273
19	24.927921200686303	26.22608061197152	28.435699098801255	20.410299088540924
20	24.65978464713024	25.959426105692774	28.183979068932295	21.196810178244686
21	22.722520848415066	27.41399850655236	27.837978031499205	22.02550261353337
22	22.496451636807212	27.171512771257404	27.90809026807955	22.423945323855833
23	22.329892323565122	26.8674650721358	28.57546755741508	22.227175046883996
24	22.82786020874066	27.238204898736267	27.777214093129572	22.1567207993935
25	22.7775846049489	26.89801804677569	28.466252073441144	21.858145274834268
26	22.68284758283789	27.01213568712842	27.93545114089139	22.369565589142294
27	22.611937320800536	26.95570388695399	27.91527249719266	22.517086295052813
28	21.994835635256766	27.18017704764782	28.35760660765078	22.467380709444633
29	23.102608973226246	27.10128653104034	27.640865743617223	22.155238752116194
30	22.720696790227606	27.1465459748166	28.21681211630651	21.915945118649287
31	22.89181624893834	26.78857455552832	28.141455712437224	22.17815348309611
32	21.69945221252558	26.846032388433187	28.303568883847397	23.150946515193834
33	21.958810486054507	26.73829895173656	28.932868958519776	22.370021603689157
34	22.16276299213945	26.65997845331266	28.26708772009827	22.91017083444962
35	22.404450701977392	27.549662834244415	27.830453791475946	22.215432672302246
36	22.779066652226206	27.1052766583254	27.849036384260657	22.266620305187736
37	23.18058746074	26.651542184195677	27.58967811073173	22.578192244332595
38	22.85989523065786	27.219736309588278	27.731270627533018	22.189097832220845
39	22.507281982295236	26.798036857375752	27.942975380914653	22.751705779414362
40	22.578762262516175	27.218026255037536	28.10383451232094	22.09937697012535
41	22.571238022492917	26.653708253293278	28.216698112669796	22.55835561154401
42	22.294551196183157	26.951827763305648	28.135299516054562	22.61832152445663
43	22.45769040032377	26.72963467534614	27.9490175736606	22.863657350669484
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	61.5
2	75.0
3	250.5
4	426.0
5	426.0
6	502.0
7	578.0
8	599.0
9	620.0
10	866.5
11	1113.0
12	1113.0
13	1780.5
14	2448.0
15	4693.0
16	6938.0
17	7740.0
18	8542.0
19	8542.0
20	8142.0
21	7742.0
22	8427.0
23	9112.0
24	11816.0
25	14520.0
26	14520.0
27	18038.0
28	21556.0
29	27316.0
30	33076.0
31	35891.5
32	38707.0
33	38707.0
34	42901.5
35	47096.0
36	50462.5
37	53829.0
38	56795.0
39	59761.0
40	59761.0
41	62476.0
42	65191.0
43	64168.0
44	63145.0
45	63793.0
46	64441.0
47	64441.0
48	63054.0
49	61667.0
50	62753.0
51	63839.0
52	63253.0
53	62667.0
54	62667.0
55	57035.5
56	51404.0
57	45757.0
58	40110.0
59	35693.0
60	31276.0
61	31276.0
62	27204.5
63	23133.0
64	18735.0
65	14337.0
66	12330.0
67	10323.0
68	10323.0
69	8673.0
70	7023.0
71	6059.0
72	5095.0
73	4132.0
74	3169.0
75	3169.0
76	2325.5
77	1482.0
78	1181.5
79	881.0
80	705.0
81	529.0
82	529.0
83	479.0
84	429.0
85	385.0
86	341.0
87	302.5
88	264.0
89	264.0
90	213.5
91	163.0
92	111.0
93	59.0
94	34.5
95	10.0
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	877165.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.04770384850367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.21449889701103	52.088636060148616
2	6.952005906154931	8.209999717993696
3	1.5924182552349644	2.8208592461419535
4	0.743612915026075	1.7563454073752882
5	0.4445866998214497	1.3125911893020283
6	0.31088810358817087	1.101433720241835
7	0.21158054798769804	0.8745341876377201
8	0.16584744493100778	0.7834328649853737
9	0.13519356040658675	0.7184582385401636
>10	0.9870322586526024	12.084164737864633
>50	0.14908552903334635	6.177335614724564
>100	0.08913591676867941	9.508128364482088
>500	0.003330352929507143	1.2269784111031252
>1k	7.836124540016808E-4	1.3371022394589445
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4843	0.5521196126156425	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3511	0.4002667685099155	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1962	0.22367513523681407	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1243	0.14170652043800197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	1.1400363671601124E-4	0.0	0.0
3	0.0	0.0	2.2800727343202248E-4	0.0	0.0
4	0.0	0.0	6.840218202960674E-4	2.2800727343202248E-4	0.0
5	0.0	0.0	6.840218202960674E-4	2.2800727343202248E-4	0.0
6	0.0	0.0	0.0015960509140241575	4.5601454686404496E-4	0.0
7	0.0	0.0	0.004218134558492416	4.5601454686404496E-4	0.0
8	0.0	0.0	0.005016160015504495	4.5601454686404496E-4	0.0
9	1.1400363671601124E-4	0.0	0.0060421927459485955	0.0011400363671601125	0.0
10	1.1400363671601124E-4	0.0	0.006840218202960675	0.0012540400038761237	0.0
11	1.1400363671601124E-4	0.0	0.009804312757576967	0.0012540400038761237	0.0
12	1.1400363671601124E-4	0.0	0.010602338214589046	0.0012540400038761237	0.0
13	1.1400363671601124E-4	0.0	0.01197038185518118	0.0012540400038761237	0.0
14	1.1400363671601124E-4	0.0	0.012540400038761236	0.0013680436405921349	0.0
15	1.1400363671601124E-4	0.0	0.014364458226217416	0.001482047277308146	0.0
16	1.1400363671601124E-4	0.0	0.015162483683229495	0.001482047277308146	0.0
17	1.1400363671601124E-4	0.0	0.015276487319945506	0.001482047277308146	0.0
18	1.1400363671601124E-4	0.0	0.015390490956661517	0.0015960509140241575	0.0
19	1.1400363671601124E-4	0.0	0.015846505503525562	0.0015960509140241575	0.0
20	1.1400363671601124E-4	0.0	0.016416523687105618	0.0017100545507401687	0.0
21	1.1400363671601124E-4	0.0	0.01653052732382163	0.0017100545507401687	0.0
22	1.1400363671601124E-4	0.0	0.01675853459725365	0.0021660690976042134	0.0
23	1.1400363671601124E-4	0.0	0.017100545507401685	0.002394076371036236	0.0
24	1.1400363671601124E-4	0.0	0.017214549144117696	0.002964094554616292	0.0
25	1.1400363671601124E-4	0.0	0.017214549144117696	0.003306105464764326	0.0
26	1.1400363671601124E-4	0.0	0.017214549144117696	0.003762120011628371	0.0
27	1.1400363671601124E-4	0.0	0.017214549144117696	0.004674149105356461	0.0
28	1.1400363671601124E-4	0.0	0.017214549144117696	0.011172356398169101	0.0
29	1.1400363671601124E-4	0.0	0.017214549144117696	0.027018861901694665	0.0
30	1.1400363671601124E-4	0.0	0.017328552780833707	0.051529643795637084	0.0
31	1.1400363671601124E-4	0.0	0.017328552780833707	0.11639771308704748	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	975	0.0	23.528204	1
GTATCAA	2695	0.0	23.133581	1
TCGCCAT	125	1.8189894E-11	20.72	13
AGCGACG	45	0.0038250962	20.555555	29
GTCGCCA	125	4.110916E-10	19.24	12
TGCTCGC	185	0.0	19.000002	10
GTACTAG	60	9.234435E-4	18.5	1
CCGTTAA	70	1.2187798E-4	18.5	16
CGGCAGC	170	0.0	18.499998	17
GCCGGCA	170	0.0	18.499998	15
CTGCTCG	325	0.0	17.646154	9
CGCCGTC	190	0.0	17.526316	26
CCGGCAG	180	0.0	17.472221	16
GAGTCGT	75	2.0665627E-4	17.266666	19
CGTCCGC	195	0.0	17.076923	29
GCCGTCC	195	0.0	17.076923	27
TTAGGCA	120	1.0403346E-7	16.958334	4
GCTTCGC	175	7.2759576E-12	16.914286	22
TTCGCCG	165	5.4569682E-11	16.818182	24
CCGTCCG	200	0.0	16.650002	28
>>END_MODULE