FastQCFastQC Report
Fri 10 Feb 2017
ERR1633391.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633391.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767968
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22070.2873817659069128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15010.1954508521188383No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9990.1300835451477145No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9890.12878140755864828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9780.12734905621067547No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8260.10755656485686904No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT8120.10573357223217633No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG8090.10534293095545648No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7900.10286886953623069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5800.027.112071
TGTATCG400.001930606623.1250025
CGCCTAT400.001930606623.12500236
TATCGCC400.001930606623.12500233
TCTATCG400.001930606623.12500231
GATACTA652.6809212E-622.7692315
ACGTATG1201.0913936E-1121.58333427
TTAACGG1800.021.58333235
GTATCAA14300.021.2167841
TAACGGC1850.021.036
TAGACTG1351.8189894E-1220.5555555
ATACAAT1800.020.5555556
CATCCGT555.1409594E-420.18181811
GACGTAT1303.274181E-1119.92307726
AACGGCC1950.019.92307737
ATTAGAG2350.018.8936183
GTATTAG2800.018.51
TATTAGA2800.018.52
GCAATTA1601.8189894E-1218.517
ATATTAG701.2185285E-418.51
Produced by FastQC (version 0.11.5)