##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633391.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767968
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74805200216676	33.0	33.0	33.0	27.0	33.0
2	31.885698362431768	33.0	33.0	33.0	33.0	33.0
3	31.604458779532482	33.0	33.0	33.0	27.0	33.0
4	31.89269604983541	33.0	33.0	33.0	33.0	33.0
5	31.999089805825243	33.0	33.0	33.0	33.0	33.0
6	35.15045548772866	37.0	37.0	37.0	33.0	37.0
7	35.293366390266264	37.0	37.0	37.0	33.0	37.0
8	35.4294970623776	37.0	37.0	37.0	33.0	37.0
9	35.55906365890245	37.0	37.0	37.0	33.0	37.0
10	35.55977462602608	37.0	37.0	37.0	33.0	37.0
11	35.57516459019126	37.0	37.0	37.0	33.0	37.0
12	35.5637500520855	37.0	37.0	37.0	33.0	37.0
13	35.57895771698821	37.0	37.0	37.0	33.0	37.0
14	35.55563773490562	37.0	37.0	37.0	33.0	37.0
15	35.57119307054461	37.0	37.0	37.0	33.0	37.0
16	35.54747593649736	37.0	37.0	37.0	33.0	37.0
17	35.53380739822492	37.0	37.0	37.0	33.0	37.0
18	35.520799043710156	37.0	37.0	37.0	33.0	37.0
19	35.51962972415517	37.0	37.0	37.0	33.0	37.0
20	35.52189544356015	37.0	37.0	37.0	33.0	37.0
21	35.53252088628693	37.0	37.0	37.0	33.0	37.0
22	35.43782423225968	37.0	37.0	37.0	33.0	37.0
23	35.48926257344056	37.0	37.0	37.0	33.0	37.0
24	35.471808721196716	37.0	37.0	37.0	33.0	37.0
25	35.49268979957498	37.0	37.0	37.0	33.0	37.0
26	35.394153141797574	37.0	37.0	37.0	33.0	37.0
27	35.40241650693779	37.0	37.0	37.0	33.0	37.0
28	35.41667881995083	37.0	37.0	37.0	33.0	37.0
29	35.42271943622651	37.0	37.0	37.0	33.0	37.0
30	35.43105962748448	37.0	37.0	37.0	33.0	37.0
31	35.42528204300179	37.0	37.0	37.0	33.0	37.0
32	35.411489801658405	37.0	37.0	37.0	33.0	37.0
33	35.40447648860369	37.0	37.0	37.0	33.0	37.0
34	35.392511146297764	37.0	37.0	37.0	33.0	37.0
35	35.33319747697821	37.0	37.0	37.0	33.0	37.0
36	35.371342295512314	37.0	37.0	37.0	33.0	37.0
37	35.36662074461436	37.0	37.0	37.0	33.0	37.0
38	35.35406813825576	37.0	37.0	37.0	33.0	37.0
39	35.25804200175007	37.0	37.0	37.0	33.0	37.0
40	35.06041137130714	37.0	37.0	37.0	27.0	37.0
41	35.22966712154673	37.0	37.0	37.0	33.0	37.0
42	35.2854181424226	37.0	37.0	37.0	33.0	37.0
43	34.96609103504313	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	28.0
16	42.0
17	28.0
18	28.0
19	20.0
20	60.0
21	132.0
22	369.0
23	938.0
24	1837.0
25	3338.0
26	5297.0
27	7876.0
28	11288.0
29	15132.0
30	19537.0
31	24962.0
32	31853.0
33	41580.0
34	60496.0
35	120998.0
36	422127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.59157673236385	20.312044251843826	13.22841576732364	24.867963248468687
2	16.3545876911538	21.02288116171507	38.407069044543526	24.21546210258761
3	18.15661590066253	25.96071711321305	29.803325138547436	26.07934184757698
4	12.77396974873953	17.359317054877287	39.04863223467645	30.81808096170674
5	14.083269094545606	36.85544189341223	35.24170277928247	13.819586232759699
6	30.569633109712907	39.50255739822492	16.649001000041668	13.278808492020499
7	27.586826534438934	31.656787782824285	22.887151547981166	17.869234134755615
8	24.99921871744656	35.82141443393475	20.130916913204715	19.048449935413974
9	25.63244822700946	15.525386474436434	20.137167173632236	38.70499812492187
10	14.963904746031083	28.027600108337847	33.48277532397183	23.525719821659237
11	34.43060127505313	23.209691028792868	23.989931872161343	18.369775823992665
12	23.575070836284844	25.546376932372183	29.671027959498314	21.20752427184466
13	27.960279594983124	22.19571648818701	26.015667319471646	23.828336597358224
14	22.38062002583441	20.586795283136798	27.134984791032963	29.897599899995836
15	24.086420267511148	28.822685320221673	24.001260469186217	23.089633943080962
16	23.5328815783991	28.352613650568774	24.45700862535939	23.657496145672734
17	22.818138255760655	27.200742739280802	25.957852410517106	24.023266594441438
18	23.511005666902786	27.045137297387388	27.2459269136214	22.19793012208842
19	24.992838243260135	25.953555356473185	26.88862035918163	22.164986041085044
20	24.196971748822868	26.81413808908705	26.816872578024086	22.172017584066005
21	23.174923955164797	26.813747447810325	27.480051252135507	22.531277344889368
22	23.101092753864744	26.678325138547436	26.979900204175173	23.240681903412643
23	23.077524063502647	26.419590399599986	27.227176132338847	23.275709404558523
24	23.183127421975914	27.171184216009	25.932070086253596	23.71361827576149
25	23.474545814408934	27.017792408016998	26.78653277219884	22.721129005375225
26	23.74109337889079	26.53834534772282	27.01505791907996	22.70550335430643
27	23.170106046085255	26.78887661985916	26.505010625442726	23.53600670861286
28	22.770480020000832	26.359431642985125	27.473931205466894	23.39615713154715
29	24.07665423559315	26.320888370348765	26.400188549522895	23.20226884453519
30	23.577805325221885	26.402532397183215	27.167668444518522	22.851993833076378
31	23.32987832826368	26.48691091295471	27.240197508229507	22.943013250552106
32	22.879338722446768	26.356566940289177	27.237072378015753	23.5270219592483
33	22.813841201716738	26.35734822284262	27.817695528980373	23.01111504646027
34	23.642391349639567	25.941575690653778	27.293845576899038	23.122187382807617
35	23.21607150297929	27.01440685028543	26.906199216634025	22.863322430101256
36	24.20764927705321	26.066841326721946	26.99656756531522	22.728941830909623
37	23.528454310596274	26.121270677944914	26.785751489645403	23.56452352181341
38	23.72781157548231	26.450320846701945	26.87481770073753	22.947049877078214
39	23.34667590316263	26.38964123505146	26.85476478186591	23.408918079919996
40	23.054085586899454	27.376010458769116	27.145792533022213	22.42411142130922
41	23.574419767490312	25.865661069211214	26.982895120630022	23.577024042668445
42	23.03767865327722	26.81622150922955	26.944482061752574	23.201617775740658
43	23.80268448685362	25.811882786782782	26.868827867827825	23.51660485853577
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	28.0
2	35.0
3	124.0
4	213.0
5	213.0
6	249.5
7	286.0
8	262.0
9	238.0
10	336.5
11	435.0
12	435.0
13	722.5
14	1010.0
15	1904.0
16	2798.0
17	3284.5
18	3771.0
19	3771.0
20	3768.5
21	3766.0
22	4190.0
23	4614.0
24	6125.5
25	7637.0
26	7637.0
27	10261.5
28	12886.0
29	16038.5
30	19191.0
31	23181.5
32	27172.0
33	27172.0
34	33229.5
35	39287.0
36	44528.0
37	49769.0
38	52267.0
39	54765.0
40	54765.0
41	57387.0
42	60009.0
43	62213.5
44	64418.0
45	62788.0
46	61158.0
47	61158.0
48	59000.0
49	56842.0
50	57941.0
51	59040.0
52	56194.0
53	53348.0
54	53348.0
55	50530.0
56	47712.0
57	41436.5
58	35161.0
59	33496.5
60	31832.0
61	31832.0
62	29217.5
63	26603.0
64	21662.5
65	16722.0
66	13768.0
67	10814.0
68	10814.0
69	8646.5
70	6479.0
71	5308.5
72	4138.0
73	3323.5
74	2509.0
75	2509.0
76	1907.0
77	1305.0
78	1025.5
79	746.0
80	613.0
81	480.0
82	480.0
83	385.0
84	290.0
85	265.0
86	240.0
87	184.5
88	129.0
89	129.0
90	104.0
91	79.0
92	46.5
93	14.0
94	10.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	767968.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.742203422002476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.58617320160332	44.61261153687696
2	8.188103769743606	8.637172693285653
3	2.4133876784451997	3.8186215161813317
4	1.1641442855554172	2.455981388853022
5	0.6988042364287201	1.8428237594940338
6	0.49946309829413427	1.5805670599207713
7	0.3275667736305002	1.2093615386376215
8	0.25161251672696394	1.0616478832588427
9	0.1970354696821984	0.935287634099548
>10	1.3850464755624172	14.339591892276763
>50	0.1768496700754927	6.488835929198644
>100	0.10455874840575201	10.110427642288053
>500	0.006753794753414701	2.4178744359517865
>1k	5.002810928455334E-4	0.48919508967693703
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2207	0.2873817659069128	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1501	0.1954508521188383	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	999	0.1300835451477145	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	989	0.12878140755864828	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	978	0.12734905621067547	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	826	0.10755656485686904	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	812	0.10573357223217633	No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG	809	0.10534293095545648	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	790	0.10286886953623069	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3021375890662112E-4	0.0
5	0.0	0.0	0.0	1.3021375890662112E-4	0.0
6	0.0	0.0	0.0	1.3021375890662112E-4	0.0
7	0.0	0.0	3.9064127671986333E-4	1.3021375890662112E-4	0.0
8	0.0	0.0	6.510687945331055E-4	1.3021375890662112E-4	0.0
9	0.0	0.0	7.812825534397267E-4	2.6042751781324224E-4	0.0
10	0.0	0.0	0.001041710071252969	2.6042751781324224E-4	0.0
11	0.0	0.0	0.0028647026959456646	2.6042751781324224E-4	0.0
12	0.0	0.0	0.0028647026959456646	2.6042751781324224E-4	0.0
13	0.0	0.0	0.0029949164548522854	2.6042751781324224E-4	0.0
14	0.0	0.0	0.003255343972665528	2.6042751781324224E-4	0.0
15	1.3021375890662112E-4	0.0	0.0033855577315721488	2.6042751781324224E-4	0.0
16	1.3021375890662112E-4	0.0	0.003906412767198633	2.6042751781324224E-4	0.0
17	1.3021375890662112E-4	0.0	0.004036626526105255	3.9064127671986333E-4	0.0
18	1.3021375890662112E-4	0.0	0.004036626526105255	3.9064127671986333E-4	0.0
19	1.3021375890662112E-4	0.0	0.004036626526105255	5.208550356264845E-4	0.0
20	1.3021375890662112E-4	0.0	0.004036626526105255	7.812825534397267E-4	0.0
21	1.3021375890662112E-4	0.0	0.004166840285011876	0.001041710071252969	0.0
22	1.3021375890662112E-4	0.0	0.0044272678028251175	0.00117192383015959	0.0
23	1.3021375890662112E-4	0.0	0.0044272678028251175	0.00117192383015959	0.0
24	1.3021375890662112E-4	0.0	0.0044272678028251175	0.0016927788657860744	0.0
25	1.3021375890662112E-4	0.0	0.0044272678028251175	0.0018229926246926956	0.0
26	1.3021375890662112E-4	0.0	0.0044272678028251175	0.002083420142505938	0.0
27	2.6042751781324224E-4	0.0	0.0044272678028251175	0.0027344889370390433	0.0
28	2.6042751781324224E-4	2.6042751781324224E-4	0.0044272678028251175	0.00820346681111713	0.0
29	2.6042751781324224E-4	2.6042751781324224E-4	0.0044272678028251175	0.019401850077086544	0.0
30	2.6042751781324224E-4	2.6042751781324224E-4	0.0044272678028251175	0.034636859869161214	0.0
31	2.6042751781324224E-4	2.6042751781324224E-4	0.0044272678028251175	0.07799804158506604	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	580	0.0	27.11207	1
TGTATCG	40	0.0019306066	23.125002	5
CGCCTAT	40	0.0019306066	23.125002	36
TATCGCC	40	0.0019306066	23.125002	33
TCTATCG	40	0.0019306066	23.125002	31
GATACTA	65	2.6809212E-6	22.76923	15
ACGTATG	120	1.0913936E-11	21.583334	27
TTAACGG	180	0.0	21.583332	35
GTATCAA	1430	0.0	21.216784	1
TAACGGC	185	0.0	21.0	36
TAGACTG	135	1.8189894E-12	20.555555	5
ATACAAT	180	0.0	20.555555	6
CATCCGT	55	5.1409594E-4	20.181818	11
GACGTAT	130	3.274181E-11	19.923077	26
AACGGCC	195	0.0	19.923077	37
ATTAGAG	235	0.0	18.893618	3
GTATTAG	280	0.0	18.5	1
TATTAGA	280	0.0	18.5	2
GCAATTA	160	1.8189894E-12	18.5	17
ATATTAG	70	1.2185285E-4	18.5	1
>>END_MODULE