FastQCFastQC Report
Fri 10 Feb 2017
ERR1633390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324659
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT125080.9442430089555124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89350.67451321434422No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC47370.35760146573571006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46890.35397789166872384No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA40520.30589004415475984No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC39890.3011341031918403No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG38990.29433990181624103No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG36890.27848676527317595No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC35660.26920135672652357No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG35530.26821997208338144No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG33810.25523549834334724No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA32900.24836580584135237No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG32010.24164709559214861No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT29910.2257939590490836No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG29380.22179292935011954No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT29340.22149096484453734No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC28290.21356439657300486No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA28260.21333792319381817No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT28240.21318694094102705No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG28180.2127339941826538No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC27520.20775157984054765No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA27480.20744961533496545No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT26540.20035344945378394No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA25770.19454063272132677No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT25350.19137000541271376No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA24910.18804839585130967No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA24440.18450031291071892No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG23840.17997084532698604No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC23660.17861200505186617No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG23080.1742335197209244No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG22900.17287467944580454No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC22290.16826972073567611No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT22250.16796775623009394No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG21950.1657030224382275No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21760.16426869103671207No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT21510.1623814128768234No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT21270.16056962584333026No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC21190.15996569683216585No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG21150.15966373232658368No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG21080.15913529444181485No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC20920.1579274364194861No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC20870.15754998078750834No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG20530.15498328249005972No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA19830.14969890364237137No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG19730.14894399237841588No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT19660.14841555449364704No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC19630.14818908111446039No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTAC19600.14796260773527375No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA19020.143584122404332No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT18850.14230077325560767No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC18600.140413495095719No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTT18550.14003603946374124No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA18220.13754483229268816No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG17990.13580853638559054No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA17920.13528009850082173No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT17800.13437420498407515No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA17360.13105259542267103No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG17120.1292408083891779No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA17010.12841040599882686No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA16940.12788196811405805No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGC16920.12773098586126694No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT16670.12584370770137823No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT16610.12539076094300494No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA16490.12448486742625837No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT16230.12252209813997414No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG16130.12176718687601866No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTT16130.12176718687601866No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT15740.11882303294659229No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC15470.11678477253391249No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC15380.11610535239635258No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG15300.11550142338518819No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC15230.11497298550041934No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT14880.11233079607657517No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAAT14810.11180235819180635No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT14700.1109719558014553No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC14540.10976409777912655No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC14530.109688606652731No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGC14340.1082542752512156No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCC14330.10817878412482006No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA14210.10727289060807348No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATT14160.10689543497609574No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG14060.10614052371214026No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGT13580.10251694964515397No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG13560.10236596739236287No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTC13550.10229047626596731No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT13260.10010123360049641No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG200.001842036837.01
TCTTACA250.005496642629.62
CTAGTGC751.4551915E-1129.69
GGCGATC250.005496642629.619
GTGACAT405.940802E-527.75000216
TGTACCT358.870112E-426.42857237
ACTAGTG1000.025.9000028
GACATCT451.3233609E-424.66666818
TAGTGCA901.4370016E-1024.66666810
ACATGGC601.3376648E-624.66666635
TTGCGGT4050.024.20987735
TGCGGTA4150.024.07228936
GCTACCG1002.0008883E-1124.0500017
GGTATCA26200.023.7958011
AGATCTT400.001931341423.1250028
GTATCAA67200.021.9136921
GCGGGCT603.7268393E-521.58333233
GCTCTCT5500.021.52727130
CGCTCTC5650.021.28318629
CACATGG705.102893E-621.14285934
Produced by FastQC (version 0.11.5)