FastQCFastQC Report
Fri 10 Feb 2017
ERR1633389.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633389.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159167
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79760.6880803197468527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56270.48543479929984207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31110.2683823814860154No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13300.1147375658554807No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12410.10705963851627937No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12090.1042990354280272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.024.3738881
GTATCAA45050.022.7913421
TTCGCCG3000.020.96666524
GCCGCTC3150.020.55555527
CGCTCTC3050.020.01639229
ACAACGC656.902196E-519.9230773
CCGCTCT3250.019.92307728
GCTTCGC3550.019.2816922
GCCGGCA3300.019.06060615
CGAGTCG1850.019.021
GAGTCGC1850.019.022
TGCTCGC3600.018.510
CTGCTCG5550.018.0000029
CGCCGTC1850.018.026
AGCTTCG3750.017.75999821
TTACACC1054.797348E-717.6190474
CCGGCAG3900.017.55128316
TTGCGGT852.7231485E-517.41176435
TAGACTG1301.3929821E-817.0769235
CCGACAA650.001579945217.07692333
Produced by FastQC (version 0.11.5)