##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633389.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1159167
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.827336354468336	33.0	33.0	33.0	27.0	33.0
2	31.947653789315947	33.0	33.0	33.0	33.0	33.0
3	31.673481042852323	33.0	33.0	33.0	27.0	33.0
4	31.952121652876592	33.0	33.0	33.0	33.0	33.0
5	32.05334002779582	33.0	33.0	33.0	33.0	33.0
6	35.21614055610624	37.0	37.0	37.0	33.0	37.0
7	35.3796959368236	37.0	37.0	37.0	33.0	37.0
8	35.50605822974602	37.0	37.0	37.0	33.0	37.0
9	35.62882569983445	37.0	37.0	37.0	33.0	37.0
10	35.639579111551654	37.0	37.0	37.0	33.0	37.0
11	35.65036271736514	37.0	37.0	37.0	33.0	37.0
12	35.642165451569966	37.0	37.0	37.0	33.0	37.0
13	35.64968982036238	37.0	37.0	37.0	33.0	37.0
14	35.63149399525694	37.0	37.0	37.0	33.0	37.0
15	35.6538816236142	37.0	37.0	37.0	33.0	37.0
16	35.61876157620084	37.0	37.0	37.0	33.0	37.0
17	35.617628003557726	37.0	37.0	37.0	33.0	37.0
18	35.59899393271203	37.0	37.0	37.0	33.0	37.0
19	35.597550654909945	37.0	37.0	37.0	33.0	37.0
20	35.60139479471034	37.0	37.0	37.0	33.0	37.0
21	35.59797164688091	37.0	37.0	37.0	33.0	37.0
22	35.50512825158066	37.0	37.0	37.0	33.0	37.0
23	35.558735712800654	37.0	37.0	37.0	33.0	37.0
24	35.54219452417124	37.0	37.0	37.0	33.0	37.0
25	35.56914922526262	37.0	37.0	37.0	33.0	37.0
26	35.44792251677282	37.0	37.0	37.0	33.0	37.0
27	35.47348828943543	37.0	37.0	37.0	33.0	37.0
28	35.48995269879146	37.0	37.0	37.0	33.0	37.0
29	35.49520043272454	37.0	37.0	37.0	33.0	37.0
30	35.49928439991822	37.0	37.0	37.0	33.0	37.0
31	35.49354320818312	37.0	37.0	37.0	33.0	37.0
32	35.47412495352266	37.0	37.0	37.0	33.0	37.0
33	35.47485565065258	37.0	37.0	37.0	33.0	37.0
34	35.44653617640944	37.0	37.0	37.0	33.0	37.0
35	35.382744677859186	37.0	37.0	37.0	33.0	37.0
36	35.40132181126619	37.0	37.0	37.0	33.0	37.0
37	35.396178462637394	37.0	37.0	37.0	33.0	37.0
38	35.376938784489205	37.0	37.0	37.0	33.0	37.0
39	35.27749409705418	37.0	37.0	37.0	33.0	37.0
40	35.046827592572946	37.0	37.0	37.0	33.0	37.0
41	35.2086118738715	37.0	37.0	37.0	33.0	37.0
42	35.25302911487301	37.0	37.0	37.0	33.0	37.0
43	34.898430510875485	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	33.0
16	52.0
17	57.0
18	48.0
19	52.0
20	55.0
21	201.0
22	477.0
23	1164.0
24	2442.0
25	4501.0
26	7385.0
27	11023.0
28	15732.0
29	21666.0
30	28600.0
31	36797.0
32	47082.0
33	62576.0
34	89564.0
35	182038.0
36	647616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16062051455916	20.02826167411598	14.260844209678156	23.550273601646698
2	15.770031410487015	22.45716104754535	39.44755156073283	22.3252559812348
3	17.88836293648801	27.882436266732917	30.78788474827182	23.441316048507247
4	12.386135906215411	18.11637149780834	39.90382748991301	29.593665106063234
5	13.81181486360464	37.066272590575814	34.79964491742778	14.322267628391769
6	28.21336356193715	40.42316594589045	17.403445750267217	13.960024741905178
7	25.740208270249237	33.056410336043044	23.173106204714248	18.030275188993475
8	24.605255325591564	35.063023705816335	20.7433441428198	19.5883768257723
9	26.58590177256599	15.357925130718868	20.111424842149578	37.94474825456556
10	15.107918013539031	28.017360742671244	33.417014114445976	23.457707129343746
11	33.13129169481188	24.367929728848388	24.064608464526682	18.436170111813052
12	23.199590740592164	26.279302292076984	30.075907958042286	20.44519900928857
13	28.48640446113459	22.70992876781344	26.423284996898634	22.380381774153335
14	22.52876419014689	21.732071392646617	28.395563365761795	27.3436010514447
15	24.154414333741386	28.35743253560531	25.45767779793593	22.030475332717373
16	22.252962687861196	28.825182221371037	25.45905809948006	23.46279699128771
17	22.26348748713516	27.33229983255217	26.389898953300083	24.01431372701259
18	23.610316718816186	26.21313408680544	28.151681336684014	22.02486785769436
19	25.27866994143208	25.784636726200798	28.42144401971416	20.515249312652966
20	25.102336419169973	25.52945347823049	28.053766195897573	21.314443906701968
21	22.97632696582977	27.071509109558846	27.68436299515083	22.267800929460552
22	22.593120749641766	26.85997789792152	27.868719520138168	22.678181832298538
23	22.478469452632797	26.52672134386158	28.608043534710703	22.38676566879492
24	22.844076824133193	26.80769897693775	27.98147290252397	22.36675129640509
25	22.92439312023203	26.576239661757107	28.503571961589657	21.995795256421204
26	22.896096938577443	26.703054866123694	27.953090452022877	22.44775774327599
27	22.934227768733926	26.551049158576806	27.920308290349883	22.594414782339385
28	22.234673692401525	26.79795059728236	28.265815020613942	22.701560689702173
29	23.179403830509322	26.662939852497523	27.754068223129195	22.403588093863956
30	22.86089924920223	26.743687492828904	28.234758235871105	22.160655022097764
31	22.996255069373092	26.44217787428386	28.147712969744653	22.413854086598395
32	21.78728345441166	26.503860099536997	28.416009082384157	23.292847363667185
33	22.050317167414185	26.53983420853078	28.957863707300156	22.451984916754878
34	22.475967656084066	26.260236876998743	28.22802926584349	23.035766201073702
35	22.35303454981034	27.110588897026915	28.062996962473914	22.47337959068883
36	22.775061746926887	26.767497694465074	28.077662666380256	22.379777892227782
37	23.133249997627605	26.375664593626286	27.75993450469173	22.731150904054374
38	22.92111490406473	26.76120006867	27.701961839838436	22.615723187426834
39	22.645744746011577	26.322695521870447	28.01787835575029	23.013681376367686
40	22.537822419030217	26.91786429392831	28.20853250653271	22.335780780508763
41	22.658857610680773	26.27334974166794	28.21043042115588	22.857362226495407
42	22.310849083867986	26.77112098601841	28.09526151106786	22.822768419045747
43	22.544810195597357	26.27421243013302	28.06316950016693	23.1178078741027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	74.5
2	101.0
3	392.0
4	683.0
5	683.0
6	862.5
7	1042.0
8	1065.5
9	1089.0
10	1430.0
11	1771.0
12	1771.0
13	2769.5
14	3768.0
15	7126.0
16	10484.0
17	11775.0
18	13066.0
19	13066.0
20	11901.0
21	10736.0
22	11204.5
23	11673.0
24	14902.5
25	18132.0
26	18132.0
27	22187.5
28	26243.0
29	32912.0
30	39581.0
31	43680.0
32	47779.0
33	47779.0
34	53093.0
35	58407.0
36	62698.5
37	66990.0
38	71102.5
39	75215.0
40	75215.0
41	78688.5
42	82162.0
43	81637.5
44	81113.0
45	82953.0
46	84793.0
47	84793.0
48	84012.5
49	83232.0
50	86047.0
51	88862.0
52	87940.0
53	87018.0
54	87018.0
55	79265.0
56	71512.0
57	64017.5
58	56523.0
59	50179.5
60	43836.0
61	43836.0
62	38089.0
63	32342.0
64	26314.0
65	20286.0
66	17410.5
67	14535.0
68	14535.0
69	12015.0
70	9495.0
71	8251.5
72	7008.0
73	5685.5
74	4363.0
75	4363.0
76	3206.5
77	2050.0
78	1574.0
79	1098.0
80	884.5
81	671.0
82	671.0
83	587.0
84	503.0
85	479.5
86	456.0
87	371.0
88	286.0
89	286.0
90	223.5
91	161.0
92	103.0
93	45.0
94	27.0
95	9.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1159167.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.13961936615668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.78899734336369	48.72301276026821
2	7.908396832450183	8.879487759405448
3	1.8533166448943528	3.1213347302799455
4	0.8243668848267339	1.8511857252894863
5	0.4886310694982464	1.3715781126054805
6	0.3258119344723521	1.0974574791737417
7	0.2228680430240701	0.8758208972976068
8	0.17344969167619823	0.7789919735903202
9	0.12938702760218676	0.6537364632454655
>10	1.0152560940144342	11.591315226991355
>50	0.1578966738771391	6.3269737170524865
>100	0.10570596351511367	11.107963667497906
>500	0.004514687020348298	1.5520051777935804
>1k	0.0010897520393944166	0.8802678314414315
>5k	3.113577255412619E-4	1.188868478067692
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7976	0.6880803197468527	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5627	0.48543479929984207	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3111	0.2683823814860154	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1330	0.1147375658554807	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1241	0.10705963851627937	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1209	0.1042990354280272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.7253769301576045E-4	0.0	0.0
2	0.0	0.0	2.588065395236407E-4	0.0	0.0
3	0.0	0.0	3.450753860315209E-4	0.0	0.0
4	0.0	0.0	4.3134423253940116E-4	0.0	0.0
5	0.0	0.0	6.901507720630418E-4	0.0	0.0
6	0.0	0.0	0.0020704523161891256	8.626884650788023E-5	0.0
7	0.0	0.0	0.004313442325394011	8.626884650788023E-5	0.0
8	0.0	0.0	0.006038819255551616	8.626884650788023E-5	0.0
9	0.0	0.0	0.00767792733920134	7.76419618570922E-4	0.0
10	3.450753860315209E-4	0.0	0.008799422343803784	9.489573115866825E-4	0.0
11	5.176130790472814E-4	0.0	0.013199133515705674	0.0010352261580945628	0.0
12	6.038819255551616E-4	0.0	0.01518331698538692	0.0012077638511103232	0.0
13	6.038819255551616E-4	0.0	0.016132274296973604	0.0012077638511103232	0.0
14	6.038819255551616E-4	0.0	0.017512575841099685	0.0013803015441260836	0.0
15	6.038819255551616E-4	0.0	0.01915168392474941	0.0013803015441260836	0.0
16	6.038819255551616E-4	0.0	0.020877060854907016	0.001552839237141844	0.0
17	6.038819255551616E-4	0.0	0.021308405087446417	0.0016391080836497243	0.0
18	6.038819255551616E-4	0.0	0.021912287013001578	0.001897914623173365	0.0
19	6.901507720630418E-4	0.0	0.02242990009204886	0.0019841834696812453	0.0
20	6.901507720630418E-4	0.0	0.02242990009204886	0.0020704523161891256	0.0
21	6.901507720630418E-4	0.0	0.0231200508641119	0.002329258855712766	0.0
22	7.76419618570922E-4	0.0	0.02337885740363554	0.002674334241744287	0.0
23	7.76419618570922E-4	0.0	0.023465126250143423	0.003105678474283688	0.0
24	7.76419618570922E-4	0.0	0.0235513950966513	0.0040546357858703705	0.0
25	7.76419618570922E-4	0.0	0.0235513950966513	0.004658517711425532	0.0
26	7.76419618570922E-4	0.0	0.0235513950966513	0.005866281562535856	0.0
27	9.489573115866825E-4	0.0	0.023637663943159182	0.006987776567138298	0.0
28	0.0010352261580945628	0.0	0.023637663943159182	0.016994962762052406	0.0
29	0.001121495004602443	8.626884650788023E-5	0.023810201636174942	0.04442845595155832	0.0
30	0.001121495004602443	8.626884650788023E-5	0.023810201636174942	0.08109271571740742	0.0
31	0.0012077638511103232	8.626884650788023E-5	0.023810201636174942	0.15476631063513713	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1685	0.0	24.373888	1
GTATCAA	4505	0.0	22.791342	1
TTCGCCG	300	0.0	20.966665	24
GCCGCTC	315	0.0	20.555555	27
CGCTCTC	305	0.0	20.016392	29
ACAACGC	65	6.902196E-5	19.923077	3
CCGCTCT	325	0.0	19.923077	28
GCTTCGC	355	0.0	19.28169	22
GCCGGCA	330	0.0	19.060606	15
CGAGTCG	185	0.0	19.0	21
GAGTCGC	185	0.0	19.0	22
TGCTCGC	360	0.0	18.5	10
CTGCTCG	555	0.0	18.000002	9
CGCCGTC	185	0.0	18.0	26
AGCTTCG	375	0.0	17.759998	21
TTACACC	105	4.797348E-7	17.619047	4
CCGGCAG	390	0.0	17.551283	16
TTGCGGT	85	2.7231485E-5	17.411764	35
TAGACTG	130	1.3929821E-8	17.076923	5
CCGACAA	65	0.0015799452	17.076923	33
>>END_MODULE