FastQCFastQC Report
Fri 10 Feb 2017
ERR1633388.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633388.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066128
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53070.49778263022826524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35820.3359821709963532No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT28540.2676976873321027No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC26010.24396695331142226No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC25530.23946467966322996No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA24590.2306477271021866No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG21720.20372788258070326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21380.20053877207990034No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC20910.1961302957993787No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT20820.19528611949034264No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC20410.191440427415845No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA19530.18318625906082572No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT18410.17268095388171026No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC18350.17211816967568622No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA18140.17014842495460206No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT17920.16808488286584725No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA17760.16658412498311648No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC15410.14454174358050814No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG14980.14050845677066917No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA14450.13553719628412347No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT13530.12690783845842152No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC12640.11855987273573154No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG12280.11518316749958729No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG12130.11377620698452717No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG12090.11340101751384449No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA12080.11330722014617381No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA11510.10796077018894541No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA11220.10524064652649588No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC10800.10130115708432759No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT10800.10130115708432759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC250.005496167629.5999988
CCCGTAT358.8689703E-426.4285721
GGTATCA11750.024.7191471
CCGCTCT1900.023.36842228
GTATCAA30850.022.6677461
CGCTCTC1900.022.39473729
GACCGCT502.7019248E-422.1999997
TTCGCCG2000.022.19999924
GCCGCTC2100.021.14285727
AGTTCCG450.003825629120.55555532
TCGCCAT3800.019.96052713
GCGGTTT656.901531E-519.92307719
GCCGGCA2050.019.8536615
TAACCGT1500.019.7333347
CGAGTCG1052.259003E-819.3809521
AACGGCC1551.8189894E-1219.09677537
TTAACGG1551.8189894E-1219.09677535
CGGCCTT4700.018.89361624
CTAACCG1601.8189894E-1218.56
TAGCGAA500.00703455718.49999810
Produced by FastQC (version 0.11.5)