##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633388.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1066128
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88634666756712	33.0	33.0	33.0	33.0	33.0
2	32.00574884066454	33.0	33.0	33.0	33.0	33.0
3	31.720106778923356	33.0	33.0	33.0	27.0	33.0
4	31.996740541473443	33.0	33.0	33.0	33.0	33.0
5	32.09773498116549	33.0	33.0	33.0	33.0	33.0
6	35.28473410322213	37.0	37.0	37.0	33.0	37.0
7	35.434231161736676	37.0	37.0	37.0	33.0	37.0
8	35.5677470247475	37.0	37.0	37.0	33.0	37.0
9	35.6784598097039	37.0	37.0	37.0	33.0	37.0
10	35.68754971260486	37.0	37.0	37.0	33.0	37.0
11	35.710842412918524	37.0	37.0	37.0	33.0	37.0
12	35.6983964402023	37.0	37.0	37.0	33.0	37.0
13	35.71178038659523	37.0	37.0	37.0	33.0	37.0
14	35.68662299461228	37.0	37.0	37.0	33.0	37.0
15	35.70631575195474	37.0	37.0	37.0	33.0	37.0
16	35.68859836717542	37.0	37.0	37.0	33.0	37.0
17	35.6868115273213	37.0	37.0	37.0	33.0	37.0
18	35.66070303003016	37.0	37.0	37.0	33.0	37.0
19	35.66666197679828	37.0	37.0	37.0	33.0	37.0
20	35.66991299356175	37.0	37.0	37.0	33.0	37.0
21	35.66475413833986	37.0	37.0	37.0	33.0	37.0
22	35.58174346795131	37.0	37.0	37.0	33.0	37.0
23	35.628234133237285	37.0	37.0	37.0	33.0	37.0
24	35.61665860009305	37.0	37.0	37.0	33.0	37.0
25	35.63515544099771	37.0	37.0	37.0	33.0	37.0
26	35.52832399111551	37.0	37.0	37.0	33.0	37.0
27	35.54604043792115	37.0	37.0	37.0	33.0	37.0
28	35.5678839689043	37.0	37.0	37.0	33.0	37.0
29	35.585243047739105	37.0	37.0	37.0	33.0	37.0
30	35.58368788738313	37.0	37.0	37.0	33.0	37.0
31	35.57198572779254	37.0	37.0	37.0	33.0	37.0
32	35.566757462518574	37.0	37.0	37.0	33.0	37.0
33	35.56413301217115	37.0	37.0	37.0	33.0	37.0
34	35.54360733420377	37.0	37.0	37.0	33.0	37.0
35	35.48670703705371	37.0	37.0	37.0	33.0	37.0
36	35.511749996248106	37.0	37.0	37.0	33.0	37.0
37	35.512211479297044	37.0	37.0	37.0	33.0	37.0
38	35.48959787192532	37.0	37.0	37.0	33.0	37.0
39	35.40023430582444	37.0	37.0	37.0	33.0	37.0
40	35.19119467831255	37.0	37.0	37.0	33.0	37.0
41	35.35115014332238	37.0	37.0	37.0	33.0	37.0
42	35.39672159440517	37.0	37.0	37.0	33.0	37.0
43	35.052201987003436	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	10.0
15	39.0
16	57.0
17	35.0
18	39.0
19	35.0
20	66.0
21	147.0
22	398.0
23	996.0
24	1977.0
25	3637.0
26	6210.0
27	9486.0
28	13599.0
29	18738.0
30	24992.0
31	31686.0
32	41154.0
33	54544.0
34	79607.0
35	163633.0
36	615043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.444085513184156	20.630168234958653	14.256824696471718	23.66892155538547
2	15.453866702684858	22.39909279185989	40.176132697011994	21.970907808443265
3	17.62780829318806	27.68992091005958	31.857806942505967	22.824463854246392
4	12.534705026038148	18.24574535140246	39.22915447300887	29.990395149550526
5	13.246158059820209	38.0344574009875	35.37155013281707	13.34783440637522
6	28.312360241922168	40.01451983251542	17.83519427310792	13.837925652454489
7	25.0287957918749	33.60712784956403	23.571747482478653	17.792328876082422
8	23.577750514009573	36.62984182011916	20.763923281257036	19.02848438461423
9	25.64410652379452	16.297574024882564	19.996285624240244	38.06203382708267
10	14.602843185808833	28.80845451953236	33.77624450347426	22.81245779118455
11	32.39817357765672	24.702943736586978	24.60886497681329	18.290017708943015
12	22.260272687707293	26.661432773550644	30.00887323098165	21.069421307760418
13	27.229938619002596	22.969380787297585	27.235472663695166	22.565207930004654
14	21.79897723350292	22.11554334939144	27.966247955217383	28.119231461888255
15	23.858017048609547	28.896717842510466	25.774391067489084	21.470874041390903
16	22.427325799528763	29.56802560292948	24.96698332657992	23.037665270961835
17	22.359228910599853	28.11369741719568	25.96179820809509	23.565275464109376
18	22.132333078204493	27.466401782900363	28.155437245809132	22.24582789308601
19	24.842795611783952	26.781399606801436	28.465156153857695	19.910648627556917
20	24.550992000960484	25.650953731634473	28.885931145228337	20.9121231221767
21	21.93582759293443	28.24313778458121	27.403369951825674	22.41766467065868
22	21.717748713100114	28.019055873215976	27.44801749883691	22.815177914847
23	21.68379406600333	27.994387165518585	28.07101961490553	22.250799153572554
24	23.520346524995123	28.005267660168386	26.92509717407291	21.549288640763585
25	23.081187249561026	26.932225774015876	28.084620233217777	21.90196674320532
26	22.594472708717902	27.176943106268663	27.618072126423844	22.61051205858959
27	22.438112496810888	26.91703060045323	27.819455074812783	22.825401827923102
28	21.148398691339125	27.845249350922213	28.272965347500488	22.73338661023817
29	23.06599207599838	27.8433734035688	27.197766121891554	21.892868398541264
30	22.05832695511233	27.982943886662763	27.77771524619933	22.181013912025573
31	22.67626401332673	27.355720889048968	27.98078654720634	21.98722855041796
32	21.400526015637897	27.43272852790659	28.112384254048294	23.054361202407218
33	21.728816802485255	26.97818648417451	28.59590968439062	22.69708702894962
34	21.6026593429682	27.421848033256797	28.096720093647292	22.878772530127716
35	22.43285984422133	27.853409721909568	27.295971965842753	22.417758468026353
36	22.553483259045816	27.896744105773415	27.161466540603	22.38830609457776
37	23.46022241231822	26.5979319556376	27.14242567496586	22.799419957078324
38	23.140467185928895	27.522211216664417	27.359566581123467	21.977755016283222
39	22.514275959359477	27.07733030180241	27.84243542989209	22.56595830894602
40	22.71397055513034	27.070389296594783	27.89289841369892	22.32274173457596
41	22.055982020920563	27.486380622214217	28.180105953506523	22.277531403358697
42	22.62036078219501	27.10650128314799	27.585336845106777	22.687801089550224
43	21.99951600558282	27.1426132697012	27.615445800129063	23.242424924586917
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	41.0
2	59.0
3	272.5
4	486.0
5	486.0
6	615.0
7	744.0
8	702.5
9	661.0
10	1013.5
11	1366.0
12	1366.0
13	2030.5
14	2695.0
15	5313.5
16	7932.0
17	8895.5
18	9859.0
19	9859.0
20	9362.0
21	8865.0
22	9772.5
23	10680.0
24	13470.0
25	16260.0
26	16260.0
27	20180.5
28	24101.0
29	33063.5
30	42026.0
31	43577.0
32	45128.0
33	45128.0
34	50842.0
35	56556.0
36	58922.5
37	61289.0
38	69505.0
39	77721.0
40	77721.0
41	84322.5
42	90924.0
43	82991.0
44	75058.0
45	76929.5
46	78801.0
47	78801.0
48	79304.5
49	79808.0
50	82937.0
51	86066.0
52	86760.0
53	87454.0
54	87454.0
55	72439.5
56	57425.0
57	49975.0
58	42525.0
59	37236.0
60	31947.0
61	31947.0
62	27917.0
63	23887.0
64	19456.0
65	15025.0
66	12927.0
67	10829.0
68	10829.0
69	9075.0
70	7321.0
71	6271.0
72	5221.0
73	4316.5
74	3412.0
75	3412.0
76	2528.5
77	1645.0
78	1249.0
79	853.0
80	679.5
81	506.0
82	506.0
83	427.0
84	348.0
85	322.0
86	296.0
87	242.5
88	189.0
89	189.0
90	147.5
91	106.0
92	67.5
93	29.0
94	15.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1066128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.67202320563587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.04408536410514	39.7547948666276
2	7.497742588474137	6.8487414698135005
3	1.8269008895604912	2.503147794672107
4	0.8239705378382151	1.5052960609962889
5	0.4851546246185047	1.1078996636948952
6	0.3173734549993191	0.8697052680949039
7	0.23726751851435196	0.7585541328071784
8	0.17772066192489466	0.6493489756443804
9	0.14082907008501203	0.578875370125571
>10	1.0624109721001467	10.060131282291088
>50	0.17875964818064877	5.750580879169438
>100	0.18063692382891153	17.704806155425814
>500	0.019838737293600642	5.948241083321501
>1k	0.0071001796629728605	5.453714738565994
>5k	2.0882881361684885E-4	0.5061622587497547
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5307	0.49778263022826524	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3582	0.3359821709963532	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2854	0.2676976873321027	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2601	0.24396695331142226	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	2553	0.23946467966322996	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2459	0.2306477271021866	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2172	0.20372788258070326	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2138	0.20053877207990034	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	2091	0.1961302957993787	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	2082	0.19528611949034264	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	2041	0.191440427415845	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1953	0.18318625906082572	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1841	0.17268095388171026	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1835	0.17211816967568622	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1814	0.17014842495460206	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1792	0.16808488286584725	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1776	0.16658412498311648	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	1541	0.14454174358050814	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1498	0.14050845677066917	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1445	0.13553719628412347	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	1353	0.12690783845842152	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	1264	0.11855987273573154	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	1228	0.11518316749958729	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	1213	0.11377620698452717	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	1209	0.11340101751384449	No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA	1208	0.11330722014617381	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	1151	0.10796077018894541	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	1122	0.10524064652649588	No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC	1080	0.10130115708432759	No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT	1080	0.10130115708432759	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.8139210301202105E-4	9.379736767067369E-5	0.0
2	9.379736767067369E-5	0.0	2.8139210301202105E-4	9.379736767067369E-5	0.0
3	9.379736767067369E-5	0.0	3.7518947068269475E-4	1.8759473534134738E-4	0.0
4	9.379736767067369E-5	0.0	6.565815736947158E-4	2.8139210301202105E-4	0.0
5	9.379736767067369E-5	0.0	0.0010317710443774105	2.8139210301202105E-4	0.0
6	9.379736767067369E-5	0.0	0.0016883526180721264	2.8139210301202105E-4	0.0
7	9.379736767067369E-5	0.0	0.004220881545180316	3.7518947068269475E-4	0.0
8	9.379736767067369E-5	0.0	0.0050650578542163795	3.7518947068269475E-4	0.0
9	2.8139210301202105E-4	0.0	0.006378221001605811	0.001219365779718758	0.0
10	3.7518947068269475E-4	0.0	0.0069410052076298535	0.0015945552504014527	0.0
11	3.7518947068269475E-4	0.0	0.010880494649798149	0.0015945552504014527	0.0
12	3.7518947068269475E-4	0.0	0.011724670958834211	0.0015945552504014527	0.0
13	3.7518947068269475E-4	0.0	0.01219365779718758	0.0015945552504014527	0.0
14	3.7518947068269475E-4	0.0	0.01322542884156499	0.0016883526180721264	0.0
15	3.7518947068269475E-4	0.0	0.014350997253613074	0.0017821499857428001	0.0
16	3.7518947068269475E-4	0.0	0.015195173562649138	0.0017821499857428001	0.0
17	3.7518947068269475E-4	0.0	0.015288970930319811	0.0018759473534134738	0.0
18	3.7518947068269475E-4	0.0	0.016226944607026547	0.0019697447210841475	0.0
19	3.7518947068269475E-4	0.0	0.016508336710038568	0.002157339456425495	0.0
20	3.7518947068269475E-4	0.0	0.016883526180721264	0.002157339456425495	0.0
21	4.6898683835336846E-4	0.0	0.017446310386745306	0.0025325289271081897	0.0
22	4.6898683835336846E-4	0.0	0.017633905122086655	0.0029077183977908845	0.0
23	4.6898683835336846E-4	0.0	0.017915297225098676	0.0036580973391562737	0.0
24	4.6898683835336846E-4	0.0	0.018009094592769347	0.004220881545180316	0.0
25	4.6898683835336846E-4	0.0	0.018009094592769347	0.004689868383533685	0.0
26	4.6898683835336846E-4	0.0	0.018009094592769347	0.0050650578542163795	0.0
27	4.6898683835336846E-4	0.0	0.018102891960440022	0.006753410472288506	0.0
28	4.6898683835336846E-4	0.0	0.018102891960440022	0.01500757882730779	0.0
29	4.6898683835336846E-4	0.0	0.018102891960440022	0.03170351027268771	0.0
30	4.6898683835336846E-4	0.0	0.018102891960440022	0.05721639427911095	0.0
31	4.6898683835336846E-4	0.0	0.018102891960440022	0.12296834901625321	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCTC	25	0.0054961676	29.599998	8
CCCGTAT	35	8.8689703E-4	26.428572	1
GGTATCA	1175	0.0	24.719147	1
CCGCTCT	190	0.0	23.368422	28
GTATCAA	3085	0.0	22.667746	1
CGCTCTC	190	0.0	22.394737	29
GACCGCT	50	2.7019248E-4	22.199999	7
TTCGCCG	200	0.0	22.199999	24
GCCGCTC	210	0.0	21.142857	27
AGTTCCG	45	0.0038256291	20.555555	32
TCGCCAT	380	0.0	19.960527	13
GCGGTTT	65	6.901531E-5	19.923077	19
GCCGGCA	205	0.0	19.85366	15
TAACCGT	150	0.0	19.733334	7
CGAGTCG	105	2.259003E-8	19.38095	21
AACGGCC	155	1.8189894E-12	19.096775	37
TTAACGG	155	1.8189894E-12	19.096775	35
CGGCCTT	470	0.0	18.893616	24
CTAACCG	160	1.8189894E-12	18.5	6
TAGCGAA	50	0.007034557	18.499998	10
>>END_MODULE