FastQCFastQC Report
Fri 10 Feb 2017
ERR1633387.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633387.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366013
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76970.5634646229574682No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC58990.43184069258491686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52450.38396413504117455No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC50620.3705674836183843No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG50260.36793207678111406No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA49420.36178279416081693No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC42740.31288135618035845No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG41540.3040966667227911No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA40810.2987526473027709No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG39460.2888698716630076No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG39020.2856488188618996No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG37770.27649810067693353No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT37640.27554642598569706No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG37120.2717397272207512No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC36860.2698363778382783No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT36400.2664689135462108No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT36290.26566365034593376No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA34980.25607369768808935No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG34450.25219379317766377No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC32610.23872393600939376No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA29920.21903159047534687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29860.21859235600246849No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG29770.21793350429315095No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT28760.2105397239996984No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT28510.20870958036270518No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC27870.20402441265200258No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG27830.20373158967008365No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA27580.20190144603309043No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG26940.19721627832238786No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG26630.1949469002125163No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC26460.19370240253936089No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT26420.193409579557442No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT25480.18652823948234754No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA25160.18418565562699624No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC25010.1830875694448003No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC24920.18242871773548275No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC24580.179939722389172No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG24440.1789148419524558No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC23470.17181388464092215No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT22880.1674947456576182No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA22620.16559139627514524No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT22370.16376125263815206No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC21790.15951531940032782No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG21630.15834402747265217No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTAC21620.15827082172717244No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA21370.15644067809017922No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT20980.15358565401646984No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA20610.15087704143371988No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTT20370.14912010354220642No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC20250.14824163459644968No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT20060.14685072543233485No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT20010.1464846967049362No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19990.14633828521397674No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT19850.14531340477726054No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC19840.14524019903178081No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG19690.1441421128495849No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT19460.14245838070355116No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG19450.14238517495807143No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG19030.13931053364792284No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT19010.13916412215696336No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC18880.1382124474657269No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA18090.13242919357282837No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG18040.13206316484542974No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA17870.13081866717227433No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG17850.13067225568131488No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA17680.1294277580081595No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGC17680.1294277580081595No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA17160.12562105924321362No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA16970.1242301500790988No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16900.12371770986074071No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTT16290.11925215938647729No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG16270.11910574789551784No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC16090.11778804447688274No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT16060.11756842724044353No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG15950.11676316404016653No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAAT15750.11529904913057196No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA15570.11398134571193685No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATT15230.11149235036562609No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCC15220.11141914462014636No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGC15030.11002823545603153No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTAC15020.10995502971055181No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGT14530.10636794818204512No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14520.1062947424365654No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCT14500.10614833094560593No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG14480.10600191945464649No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT14100.10322010112641682No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCT14060.1029272781444979No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13920.1019023977077817No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTC13850.10138995748942359No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAG13730.10051148854366686No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA13680.10014545981626823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG308.313138E-637.01
AGCGGAA200.001842057637.018
GCGGAAT200.001842057637.019
TCTAGTA250.005496700829.63
CGTAGCG250.005496700829.615
CGCGTAA250.005496700829.637
CCCAACG358.870254E-426.42857221
CTGGTAT659.3945346E-825.61538533
GGTATCA18400.025.135871
GCTATAC451.3233909E-424.6666663
GGTCCGG601.3377157E-624.66666622
CCCTTAG451.3233909E-424.6666661
GCCGGCA3850.024.50649315
CCGCTCT3650.024.32876828
GCCGCTC3650.024.32876827
CCGGCAG3900.024.19230816
CGGTCCG701.9234722E-723.78571521
CGCTCTC3550.023.45070529
TGAAAGA400.001931372723.1254
AGCGGTC400.001931372723.12519
Produced by FastQC (version 0.11.5)