FastQCFastQC Report
Fri 10 Feb 2017
ERR1633386.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633386.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005127
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55570.5528654587927695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38610.3841305626055215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22640.2252451680235433No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12600.12535729315797906No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11770.11709963019598518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.025.207171
CGTTCGT400.001931019423.12524
GTATCAA31950.022.7558691
TTGCGGT555.142477E-420.18181835
CGAGTCG1052.2586391E-819.3809521
CGCCGTC1052.2586391E-819.3809526
CGTAGTT500.00703428418.49999828
GCCGCTC1156.402479E-817.6956527
TTCGCCG1054.795784E-717.61904724
ATAAGGT752.0669404E-417.2666664
GTCGGCT752.0669404E-417.26666623
GTACCGG650.001579747217.0769236
GCGATAG1107.8051926E-716.81818236
CGATAGA1107.8051926E-716.81818237
GAGTCGC1352.2180757E-816.44444522
TAGCTCG904.4450622E-516.44444536
ATAGGCG1151.2417386E-616.08695632
TAGTACC2200.015.9772734
CTCGGTA700.002592190415.85714227
ATTTCGG1059.340683E-615.85714229
Produced by FastQC (version 0.11.5)