##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633386.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1005127
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61523369683632	33.0	33.0	33.0	27.0	33.0
2	31.791730796207844	33.0	33.0	33.0	27.0	33.0
3	31.520040751069267	33.0	33.0	33.0	27.0	33.0
4	31.821425551199003	33.0	33.0	33.0	27.0	33.0
5	31.92967256874007	33.0	33.0	33.0	33.0	33.0
6	35.02585046466765	37.0	37.0	37.0	33.0	37.0
7	35.191664336944484	37.0	37.0	37.0	33.0	37.0
8	35.33090843246674	37.0	37.0	37.0	33.0	37.0
9	35.46301412657306	37.0	37.0	37.0	33.0	37.0
10	35.45901164728437	37.0	37.0	37.0	33.0	37.0
11	35.47370431796181	37.0	37.0	37.0	33.0	37.0
12	35.47161602464166	37.0	37.0	37.0	33.0	37.0
13	35.476975546373744	37.0	37.0	37.0	33.0	37.0
14	35.4648099195425	37.0	37.0	37.0	33.0	37.0
15	35.487386171100766	37.0	37.0	37.0	33.0	37.0
16	35.457933176603554	37.0	37.0	37.0	33.0	37.0
17	35.441694432643835	37.0	37.0	37.0	33.0	37.0
18	35.42864235066813	37.0	37.0	37.0	33.0	37.0
19	35.414535675591246	37.0	37.0	37.0	33.0	37.0
20	35.41843269556981	37.0	37.0	37.0	33.0	37.0
21	35.40361665739752	37.0	37.0	37.0	33.0	37.0
22	35.306726413677076	37.0	37.0	37.0	33.0	37.0
23	35.3624457406875	37.0	37.0	37.0	33.0	37.0
24	35.3513207783693	37.0	37.0	37.0	33.0	37.0
25	35.36934934590355	37.0	37.0	37.0	33.0	37.0
26	35.248444226450985	37.0	37.0	37.0	33.0	37.0
27	35.269815655136114	37.0	37.0	37.0	33.0	37.0
28	35.2806461273053	37.0	37.0	37.0	33.0	37.0
29	35.29503037924561	37.0	37.0	37.0	33.0	37.0
30	35.29581833937403	37.0	37.0	37.0	33.0	37.0
31	35.290962236612884	37.0	37.0	37.0	33.0	37.0
32	35.27376043027399	37.0	37.0	37.0	33.0	37.0
33	35.27637900484218	37.0	37.0	37.0	33.0	37.0
34	35.244362155230135	37.0	37.0	37.0	33.0	37.0
35	35.17867592851451	37.0	37.0	37.0	33.0	37.0
36	35.2060296858009	37.0	37.0	37.0	33.0	37.0
37	35.20211575253674	37.0	37.0	37.0	33.0	37.0
38	35.18207748871536	37.0	37.0	37.0	33.0	37.0
39	35.077719531959644	37.0	37.0	37.0	33.0	37.0
40	34.870331808816196	37.0	37.0	37.0	27.0	37.0
41	35.04255681122883	37.0	37.0	37.0	27.0	37.0
42	35.08293180861722	37.0	37.0	37.0	33.0	37.0
43	34.711073327052205	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	39.0
16	52.0
17	49.0
18	32.0
19	36.0
20	70.0
21	226.0
22	579.0
23	1408.0
24	2774.0
25	4809.0
26	7936.0
27	11713.0
28	16004.0
29	21394.0
30	27687.0
31	35724.0
32	45029.0
33	58396.0
34	83328.0
35	164958.0
36	522882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.12243825904587	20.116661874569083	13.870883977845585	23.89001588853946
2	16.181835728221408	21.97980951660835	38.92294207597647	22.91541267919377
3	18.32753472944215	27.243025010769784	30.245431671818583	24.18400858796948
4	12.381917906891369	17.92271026447404	40.09622664598603	29.599145182648563
5	14.035838257255055	36.7158577970744	34.99816441106447	14.250139534606074
6	28.8723713520779	40.31241823172594	17.125199104192802	13.690011312003358
7	26.392983175260436	32.59309520090496	22.919989215293192	18.09393240854141
8	25.007884575779975	34.77162587414327	20.68285898199929	19.537630568077464
9	26.6165370147255	15.170819209910787	20.209983415031136	38.00266036033258
10	15.185344737530679	28.007903478863867	33.314397086139365	23.49235469746609
11	33.69415009247588	24.15774325035543	23.97119965934653	18.176906997822165
12	23.644474777814146	25.82837790647351	30.05938553038571	20.46776178532663
13	28.834963143960913	22.462136625520955	25.881903480853662	22.82099674966447
14	22.41428197630747	21.402171068929597	28.319406403369925	27.864140551393007
15	24.052681899899216	28.497592841501625	25.011267232896937	22.438458025702225
16	22.474274395175932	28.51749082454257	25.37778808051122	23.63044669977028
17	22.397169710892257	27.31316540098913	26.356470376380297	23.933194511738318
18	23.74247234429082	26.164753309780753	27.97527078667671	22.117503559251716
19	25.548612264917768	25.79962532097934	27.74763786068825	20.904124553414643
20	25.312920655797726	25.646908301140055	27.556020284003914	21.484150759058306
21	23.726354978027654	26.568582875596814	27.588056036699836	22.117006109675692
22	22.805973772468555	26.7108534543396	27.868816577407628	22.614356195784215
23	22.93252494460899	26.302745822169737	28.178329703609595	22.586399529611683
24	22.7859762995124	26.522817514602632	27.76683941432277	22.9243667715622
25	23.061861834375158	26.837902076056057	27.857375237159086	22.242860852409695
26	23.227612033106265	26.69045802172263	27.688142891395813	22.393787053775295
27	23.158466542038965	26.50092973325759	27.76693890423797	22.57366482046547
28	22.687580773374908	26.627182435652408	27.859961974954413	22.825274816018272
29	23.306507535863627	26.704486099766495	27.398129788573982	22.590876575795896
30	23.351576467451377	26.56470276890383	27.824444075226314	22.259276688418478
31	23.22084671887234	26.48570777623126	27.606063711351897	22.687381793544496
32	22.068355541140573	26.508590456728353	28.139130677018926	23.28392332511215
33	22.265544553076378	26.557539495009085	28.541069934446096	22.635846017468438
34	22.79124926501825	26.186541601210596	27.94422993313283	23.077979200638328
35	22.606993942059063	26.789052527690533	27.78892617549822	22.815027354752186
36	23.181050752790444	26.656134000976987	27.653022951328538	22.509792294904027
37	23.337349409577097	26.170921684523446	27.61203310626418	22.879695799635268
38	23.29208149815894	26.42024341202654	27.505877366740723	22.7817977230738
39	22.823384507629385	26.393779094582076	27.82334968615906	22.959486711629477
40	22.74488696453284	26.773631590833798	27.92711766771761	22.554363776915753
41	22.866861600573856	25.87265091873962	28.041929029863887	23.21855845082263
42	22.32513901228402	26.674241165544256	28.124505659483827	22.876114162687898
43	22.844575859567996	26.241758504149225	27.886923741974893	23.026741894307882
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	57.0
1	71.5
2	86.0
3	285.0
4	484.0
5	484.0
6	629.0
7	774.0
8	769.5
9	765.0
10	993.5
11	1222.0
12	1222.0
13	1980.5
14	2739.0
15	4985.0
16	7231.0
17	8401.0
18	9571.0
19	9571.0
20	9086.5
21	8602.0
22	9520.5
23	10439.0
24	13341.0
25	16243.0
26	16243.0
27	20217.5
28	24192.0
29	28202.0
30	32212.0
31	36945.0
32	41678.0
33	41678.0
34	46919.0
35	52160.0
36	55398.0
37	58636.0
38	61669.0
39	64702.0
40	64702.0
41	66325.0
42	67948.0
43	69013.5
44	70079.0
45	70615.5
46	71152.0
47	71152.0
48	71702.0
49	72252.0
50	72056.5
51	71861.0
52	71316.5
53	70772.0
54	70772.0
55	67155.0
56	63538.0
57	57425.0
58	51312.0
59	46688.5
60	42065.0
61	42065.0
62	37052.5
63	32040.0
64	26655.5
65	21271.0
66	17878.5
67	14486.0
68	14486.0
69	12145.5
70	9805.0
71	8256.0
72	6707.0
73	5042.5
74	3378.0
75	3378.0
76	2572.0
77	1766.0
78	1382.0
79	998.0
80	811.5
81	625.0
82	625.0
83	546.5
84	468.0
85	418.0
86	368.0
87	313.0
88	258.0
89	258.0
90	197.5
91	137.0
92	89.5
93	42.0
94	24.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1005127.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.81893437520062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.4557430268316	54.06420838910111
2	7.87043522765453	9.73083837685285
3	1.7390874798317535	3.2252560436535656
4	0.7052758102569509	1.7439759612276353
5	0.4106858369085014	1.2694080400335495
6	0.27596307385504726	1.0235845891574284
7	0.20678447555328477	0.8948237146827153
8	0.15531983022755344	0.7681365113603541
9	0.11710622236502943	0.6515443687780265
>10	0.8657208785060229	10.701303323638527
>50	0.12382385492783442	5.311203674176014
>100	0.0701395194122238	7.97108994148025
>500	0.0030991879052780593	1.2209228853246001
>1k	6.524606116374862E-4	0.8633582543895959
>5k	1.6311515290937155E-4	0.5603459261437729
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5557	0.5528654587927695	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3861	0.3841305626055215	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2264	0.2252451680235433	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1260	0.12535729315797906	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1177	0.11709963019598518	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.9897983040949055E-4	0.0	0.0
2	0.0	0.0	1.9897983040949055E-4	0.0	0.0
3	0.0	0.0	2.984697456142358E-4	0.0	0.0
4	0.0	0.0	2.984697456142358E-4	0.0	0.0
5	0.0	0.0	2.984697456142358E-4	0.0	0.0
6	9.948991520474528E-5	0.0	0.0012933688976616885	0.0	0.0
7	9.948991520474528E-5	0.0	0.002188778134504396	9.948991520474528E-5	0.0
8	9.948991520474528E-5	0.0	0.003183677286551849	1.9897983040949055E-4	0.0
9	9.948991520474528E-5	0.0	0.004178576438599302	5.969394912284716E-4	0.0
10	1.9897983040949055E-4	0.0	0.004576536099418283	5.969394912284716E-4	0.0
11	1.9897983040949055E-4	0.0	0.0068648041491274235	6.964294064332169E-4	0.0
12	1.9897983040949055E-4	0.0	0.007859703301174876	6.964294064332169E-4	0.0
13	1.9897983040949055E-4	0.0	0.008755112538017584	7.959193216379622E-4	0.0
14	1.9897983040949055E-4	0.0	0.00925256211404131	8.954092368427075E-4	0.0
15	1.9897983040949055E-4	0.0	0.009650521774860291	8.954092368427075E-4	0.0
16	2.984697456142358E-4	0.0	0.010446441096498253	9.948991520474528E-4	0.0
17	3.979596608189811E-4	0.0	0.01094389067252198	0.001094389067252198	0.0
18	3.979596608189811E-4	0.0	0.01114287050293147	0.0011938789824569433	0.0
19	3.979596608189811E-4	0.0	0.011640320078955197	0.0012933688976616885	0.0
20	3.979596608189811E-4	0.0	0.011640320078955197	0.0013928588128664337	0.0
21	3.979596608189811E-4	0.0	0.012038279739774178	0.0018903083888901601	0.0
22	3.979596608189811E-4	0.0	0.012237259570183668	0.0024872478801186318	0.0
23	3.979596608189811E-4	0.0	0.012336749485388413	0.002984697456142358	0.0
24	4.974495760237264E-4	0.0	0.012336749485388413	0.0034821470321660846	0.0
25	4.974495760237264E-4	0.0	0.012336749485388413	0.004079086523394556	0.0
26	5.969394912284716E-4	0.0	0.012336749485388413	0.004676026014623027	0.0
27	5.969394912284716E-4	0.0	0.012336749485388413	0.0057704150818752254	0.0
28	5.969394912284716E-4	0.0	0.012336749485388413	0.0147245074503023	0.0
29	5.969394912284716E-4	0.0	0.012336749485388413	0.034025551000022886	0.0
30	5.969394912284716E-4	0.0	0.012336749485388413	0.06208170708776105	0.0
31	5.969394912284716E-4	0.0	0.012336749485388413	0.1263521923100265	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1255	0.0	25.20717	1
CGTTCGT	40	0.0019310194	23.125	24
GTATCAA	3195	0.0	22.755869	1
TTGCGGT	55	5.142477E-4	20.181818	35
CGAGTCG	105	2.2586391E-8	19.38095	21
CGCCGTC	105	2.2586391E-8	19.38095	26
CGTAGTT	50	0.007034284	18.499998	28
GCCGCTC	115	6.402479E-8	17.69565	27
TTCGCCG	105	4.795784E-7	17.619047	24
ATAAGGT	75	2.0669404E-4	17.266666	4
GTCGGCT	75	2.0669404E-4	17.266666	23
GTACCGG	65	0.0015797472	17.076923	6
GCGATAG	110	7.8051926E-7	16.818182	36
CGATAGA	110	7.8051926E-7	16.818182	37
GAGTCGC	135	2.2180757E-8	16.444445	22
TAGCTCG	90	4.4450622E-5	16.444445	36
ATAGGCG	115	1.2417386E-6	16.086956	32
TAGTACC	220	0.0	15.977273	4
CTCGGTA	70	0.0025921904	15.857142	27
ATTTCGG	105	9.340683E-6	15.857142	29
>>END_MODULE