FastQCFastQC Report
Fri 10 Feb 2017
ERR1633385.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633385.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193843
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78000.6533522414588853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55730.4668118002115856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32100.2688795762926951No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA27660.23168875639426625No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15890.13309957842027803No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14430.12087016466989378No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14330.12003253359110033No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA13000.10889204024314755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGT200.001841964137.06
GGTATCA18100.024.6325971
GTATCAA46450.022.3035531
GCTTTAT5850.022.1367511
TTCGCCG2550.021.03921524
TGGACCG806.960581E-720.81255
CGCTCTC2500.020.7229
TATTGAG6550.020.3358765
GCCGGCA2750.020.18181815
GACCGTG902.1523574E-618.57
CCGCTCT2800.018.528
GCGTTAG500.007035037518.523
TTATTGA7250.018.117244
CTTTATT8550.017.7426912
GCCGCTC2950.017.55932427
TTAACGG953.6066212E-617.52631635
ATTGAGC7000.017.4428566
GGACCGT1401.869921E-917.1785726
AGCTTCG3800.017.03947421
TAATACG1655.4569682E-1116.8181822
Produced by FastQC (version 0.11.5)