##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633385.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1193843
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92007994351016	33.0	33.0	33.0	33.0	33.0
2	32.02532828856056	33.0	33.0	33.0	33.0	33.0
3	31.73698384125886	33.0	33.0	33.0	27.0	33.0
4	32.01396582297672	33.0	33.0	33.0	33.0	33.0
5	32.109282376325865	33.0	33.0	33.0	33.0	33.0
6	35.287412163911	37.0	37.0	37.0	33.0	37.0
7	35.44060651191153	37.0	37.0	37.0	33.0	37.0
8	35.578545085074	37.0	37.0	37.0	33.0	37.0
9	35.688202720123165	37.0	37.0	37.0	33.0	37.0
10	35.6805978675588	37.0	37.0	37.0	33.0	37.0
11	35.72056627211451	37.0	37.0	37.0	33.0	37.0
12	35.69364062108669	37.0	37.0	37.0	33.0	37.0
13	35.71244208828129	37.0	37.0	37.0	33.0	37.0
14	35.68729053987836	37.0	37.0	37.0	33.0	37.0
15	35.71786239899216	37.0	37.0	37.0	33.0	37.0
16	35.69221581062166	37.0	37.0	37.0	33.0	37.0
17	35.6795826586913	37.0	37.0	37.0	33.0	37.0
18	35.67125995629241	37.0	37.0	37.0	33.0	37.0
19	35.664776691742546	37.0	37.0	37.0	33.0	37.0
20	35.657939946877434	37.0	37.0	37.0	33.0	37.0
21	35.6533396769927	37.0	37.0	37.0	33.0	37.0
22	35.5604112098492	37.0	37.0	37.0	33.0	37.0
23	35.609471262134136	37.0	37.0	37.0	33.0	37.0
24	35.6097175256713	37.0	37.0	37.0	33.0	37.0
25	35.62582349605434	37.0	37.0	37.0	33.0	37.0
26	35.52099480417442	37.0	37.0	37.0	33.0	37.0
27	35.53511140074532	37.0	37.0	37.0	33.0	37.0
28	35.548010919358745	37.0	37.0	37.0	33.0	37.0
29	35.558965458607204	37.0	37.0	37.0	33.0	37.0
30	35.56454910737844	37.0	37.0	37.0	33.0	37.0
31	35.55212368795562	37.0	37.0	37.0	33.0	37.0
32	35.548066203009945	37.0	37.0	37.0	33.0	37.0
33	35.53684194655411	37.0	37.0	37.0	33.0	37.0
34	35.51116771635802	37.0	37.0	37.0	33.0	37.0
35	35.453006802401994	37.0	37.0	37.0	33.0	37.0
36	35.47327496161556	37.0	37.0	37.0	33.0	37.0
37	35.46595490361798	37.0	37.0	37.0	33.0	37.0
38	35.447979340667075	37.0	37.0	37.0	33.0	37.0
39	35.348679851538265	37.0	37.0	37.0	33.0	37.0
40	35.13046104052208	37.0	37.0	37.0	33.0	37.0
41	35.2929380161378	37.0	37.0	37.0	33.0	37.0
42	35.330580319187696	37.0	37.0	37.0	33.0	37.0
43	34.99491139119633	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	33.0
16	70.0
17	45.0
18	41.0
19	24.0
20	64.0
21	161.0
22	446.0
23	1060.0
24	2246.0
25	4193.0
26	7132.0
27	10678.0
28	15505.0
29	21125.0
30	27990.0
31	36145.0
32	46788.0
33	61713.0
34	89640.0
35	183668.0
36	685073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85571888430891	19.634407539349812	13.954766246482997	23.55510732985828
2	16.000261340896586	21.86409770799008	39.11552859128043	23.020112359832908
3	18.37678823764934	27.37328107632243	30.29334678010425	23.956583905923978
4	12.20403352869682	17.67007889647131	40.27330226838872	29.852585306443142
5	14.049251032170899	36.56929763796412	34.82233426003252	14.559117069832467
6	28.9228985720903	40.17421051176746	16.974845101072756	13.928045815069487
7	26.34567526885864	32.563410766742365	22.879306575487732	18.211607388911272
8	25.11368747816924	34.282481029750144	20.816053702203725	19.78777778987689
9	26.905380355708413	15.041006229462333	20.064698624526006	37.98891479030325
10	15.428996945159456	27.896632974352574	33.035583405858226	23.638786674629745
11	33.858220888341265	24.27856929261218	23.55150551621947	18.31170430282709
12	23.962698612799173	25.817800163003007	29.637900460948387	20.581600763249437
13	29.26791881344532	22.389711209932965	25.585357538637826	22.75701243798389
14	22.5128429785156	21.359843798556426	28.426099579257908	27.701213643670066
15	24.352197064438123	28.41981734616696	24.970117511264046	22.257868078130876
16	22.22444659808702	28.37592547763818	25.546826509013332	23.85280141526147
17	22.619054599306608	26.939639466831068	26.23728580726276	24.204020126599563
18	24.09219637758064	25.6454994500952	28.063572848356106	22.19873132396806
19	25.99353516333387	25.46356597978126	27.81261857714959	20.730280279735275
20	25.74433991739282	25.202141320089826	27.29370612383705	21.759812638680295
21	23.797685290276863	26.28117767579154	27.624067821313186	22.29706921261841
22	23.08268340141878	26.480282583220742	27.99254173287442	22.444492282486056
23	23.03426832506452	26.005597050868495	28.210912155115874	22.74922246895111
24	22.827876027249815	26.233348941192435	27.952921782847497	22.98585324871026
25	23.159912986883533	26.640102593054532	27.955602202299634	22.2443822177623
26	23.35080910974056	26.335121117265835	27.69560151544215	22.618468257551452
27	23.361363261333356	26.005764577084257	27.91606601538058	22.716806146201804
28	22.847225305169943	26.36494078367089	27.801813136233157	22.986020774926015
29	23.408186838637913	26.48597847455654	27.477566145632217	22.628268541173338
30	23.722298493185452	26.142884784682742	27.7168773448435	22.417939377288302
31	23.106807176488033	26.318871074337245	27.82300520252663	22.75131654664809
32	22.091765835206136	26.35916112922721	28.178328306150807	23.370744729415843
33	22.1243496841712	26.486146000772298	28.478619047898256	22.910885267158246
34	23.027567276434173	25.858844085863886	27.99538967854232	23.11819895915962
35	22.70080739259685	26.504071305858474	27.626999530088963	23.16812177145571
36	23.035776061006345	26.48723492117473	28.04305088692567	22.433938130893257
37	23.447806788664842	25.99378645265751	27.835150853169132	22.723255905508513
38	23.38272285384259	26.268864498933276	27.42764333333613	22.920769313888005
39	23.167619192808434	25.931801752826793	27.771239601857197	23.129339452507576
40	22.80777288135877	26.353213948567777	28.085686308836255	22.7533268612372
41	22.93819204032691	25.673308801911137	27.982657686144663	23.40584147161729
42	22.405039858674886	26.40506331234509	28.100596142038775	23.089300686941247
43	22.99841771489216	26.285282068077624	27.759429003646208	22.956871213384
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	66.0
1	94.5
2	123.0
3	446.0
4	769.0
5	769.0
6	977.5
7	1186.0
8	1096.5
9	1007.0
10	1407.0
11	1807.0
12	1807.0
13	2860.5
14	3914.0
15	6951.0
16	9988.0
17	11409.0
18	12830.0
19	12830.0
20	11828.0
21	10826.0
22	11545.0
23	12264.0
24	15665.0
25	19066.0
26	19066.0
27	23397.0
28	27728.0
29	32546.5
30	37365.0
31	42145.0
32	46925.0
33	46925.0
34	53203.5
35	59482.0
36	62459.5
37	65437.0
38	69195.0
39	72953.0
40	72953.0
41	75237.5
42	77522.0
43	79037.5
44	80553.0
45	82129.5
46	83706.0
47	83706.0
48	85595.0
49	87484.0
50	87456.5
51	87429.0
52	86266.0
53	85103.0
54	85103.0
55	80952.5
56	76802.0
57	68805.0
58	60808.0
59	56753.0
60	52698.0
61	52698.0
62	46566.0
63	40434.0
64	33808.0
65	27182.0
66	23066.0
67	18950.0
68	18950.0
69	15967.5
70	12985.0
71	11012.0
72	9039.0
73	6666.5
74	4294.0
75	4294.0
76	3106.5
77	1919.0
78	1483.0
79	1047.0
80	876.0
81	705.0
82	705.0
83	602.0
84	499.0
85	468.0
86	437.0
87	360.0
88	283.0
89	283.0
90	223.0
91	163.0
92	108.5
93	54.0
94	32.0
95	10.0
96	10.0
97	5.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1193843.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.506932130129165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.685605456973	50.71708835130272
2	8.497021540067248	9.9426932510592
3	1.7340794066852505	3.0436699846556583
4	0.7144856204807448	1.672094468216806
5	0.4537564601169835	1.3273949207836007
6	0.27872252703172384	0.9784319983309894
7	0.20421568582444882	0.8363623289307164
8	0.16602388255209322	0.7770838422764682
9	0.12628085277433104	0.6649474754342357
>10	0.916813848238718	10.670582455050292
>50	0.1300438629395427	5.325492988429003
>100	0.08597954459479604	9.677144168457986
>500	0.005373719451616282	1.9587109984780877
>1k	0.0013071209476904469	1.27195978205798
>5k	2.904713217089882E-4	1.1363429865362662
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7800	0.6533522414588853	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5573	0.4668118002115856	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3210	0.2688795762926951	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2766	0.23168875639426625	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1589	0.13309957842027803	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1443	0.12087016466989378	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1433	0.12003253359110033	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1300	0.10889204024314755	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.350524315173771E-4	0.0	0.0
2	0.0	0.0	4.1881553939672136E-4	8.376310787934427E-5	0.0
3	0.0	0.0	5.863417551554099E-4	8.376310787934427E-5	0.0
4	0.0	0.0	9.21394186672787E-4	1.6752621575868854E-4	0.0
5	0.0	0.0	0.001256446618190164	1.6752621575868854E-4	0.0
6	0.0	0.0	0.0017590252654662297	1.6752621575868854E-4	0.0
7	0.0	0.0	0.004523207825484591	2.512893236380328E-4	0.0
8	0.0	0.0	0.005444602012157377	2.512893236380328E-4	0.0
9	8.376310787934427E-5	0.0	0.006784811738226886	7.538679709140984E-4	0.0
10	4.1881553939672136E-4	0.0	0.007622442817020328	8.376310787934427E-4	0.0
11	4.1881553939672136E-4	0.0	0.011559308887349509	9.21394186672787E-4	0.0
12	4.1881553939672136E-4	0.0	0.012396939966142951	9.21394186672787E-4	0.0
13	4.1881553939672136E-4	0.0	0.013402097260695084	9.21394186672787E-4	0.0
14	4.1881553939672136E-4	0.0	0.014407254555247214	0.0010051572945521312	0.0
15	4.1881553939672136E-4	0.0	0.015579938065558034	0.0010051572945521312	0.0
16	5.025786472760656E-4	0.0	0.016417569144351475	0.0010051572945521312	0.0
17	5.025786472760656E-4	0.0	0.01666885846798951	0.0011726835103108197	0.0
18	5.025786472760656E-4	0.0	0.016836384683748196	0.001256446618190164	0.0
19	5.025786472760656E-4	0.0	0.017422726438903607	0.0015077359418281969	0.0
20	5.025786472760656E-4	0.0	0.017590252654662297	0.0015914990497075412	0.0
21	5.025786472760656E-4	0.0	0.018092831301938363	0.0019265514812249183	0.0
22	5.025786472760656E-4	0.0	0.018511646841335085	0.0022616039127422954	0.0
23	5.025786472760656E-4	0.0	0.018595409949214426	0.002596656344259672	0.0
24	5.863417551554099E-4	0.0	0.018762936164973116	0.003350524315173771	0.0
25	5.863417551554099E-4	0.0	0.018762936164973116	0.003769339854570492	0.0
26	5.863417551554099E-4	0.0	0.018762936164973116	0.004271918501846558	0.0
27	5.863417551554099E-4	0.0	0.018762936164973116	0.0058634175515540985	0.0
28	5.863417551554099E-4	0.0	0.018762936164973116	0.015244885634040656	0.0
29	5.863417551554099E-4	0.0	0.018762936164973116	0.038195977192980984	0.0
30	5.863417551554099E-4	0.0	0.01884669927285246	0.06969090575561443	0.0
31	6.701048630347542E-4	0.0	0.01884669927285246	0.14675296500461116	8.376310787934427E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACGT	20	0.0018419641	37.0	6
GGTATCA	1810	0.0	24.632597	1
GTATCAA	4645	0.0	22.303553	1
GCTTTAT	585	0.0	22.136751	1
TTCGCCG	255	0.0	21.039215	24
TGGACCG	80	6.960581E-7	20.8125	5
CGCTCTC	250	0.0	20.72	29
TATTGAG	655	0.0	20.335876	5
GCCGGCA	275	0.0	20.181818	15
GACCGTG	90	2.1523574E-6	18.5	7
CCGCTCT	280	0.0	18.5	28
GCGTTAG	50	0.0070350375	18.5	23
TTATTGA	725	0.0	18.11724	4
CTTTATT	855	0.0	17.742691	2
GCCGCTC	295	0.0	17.559324	27
TTAACGG	95	3.6066212E-6	17.526316	35
ATTGAGC	700	0.0	17.442856	6
GGACCGT	140	1.869921E-9	17.178572	6
AGCTTCG	380	0.0	17.039474	21
TAATACG	165	5.4569682E-11	16.818182	2
>>END_MODULE