FastQCFastQC Report
Fri 10 Feb 2017
ERR1633384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032425
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT33480.3242850570259341No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG28400.2750805143230743No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC25950.25134997699590766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25150.24360123011356757No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC24790.24011429401651452No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC24530.23759595127975397No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT21740.2105721965275928No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT20520.1987553575320241No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC20350.19710874881952684No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC19850.19226578201806427No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT18500.17918977165411532No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA18100.17531539821294526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16140.15633096835121194No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA16020.15516865631886093No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT14620.14160834927476573No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA14570.14112405259461946No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA14440.1398648812262392No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG13380.12959779160713855No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG13170.12756374555052424No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA12840.12436738746155894No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC12380.11991185800421338No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA12190.11807153061965761No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG12040.11661864057921884No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT11920.11545632854686781No Hit
CTGTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGC11490.11129137709760999No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC11460.11100079908952223No Hit
ATGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAA11320.10964476838511272No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG11270.10916047170496646No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA11050.10702956631232292No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC11000.10654526963217667No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10810.10470494224762089No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG10770.1043175049035039No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA10470.10141172482262635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10340.10015255345424608No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA10330.10005569411821681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAC358.8687823E-426.4285711
GGTATCA7250.025.0068951
GTATCAA18750.022.1013341
CGCTCTC1059.822543E-1021.14285729
CTTATCG450.003825548320.55555519
ATCGCTC450.003825548320.5555558
TTACCGA555.1426067E-420.18181810
GCCGGCA1052.258821E-819.3809515
CCGCTCT1052.258821E-819.3809528
AGATCGC701.21904486E-418.535
CTATCGC902.1517844E-618.532
CGCCTAT902.1517844E-618.536
TACTATC801.6164102E-518.57
TATCGCC902.1517844E-618.533
ACCGACG500.007034410318.49999812
TCGCTCA500.007034410318.4999989
CCGTTAA1653.6379788E-1217.93939416
GCTTCGC1258.574716E-917.76000222
ATTAATC1156.4030246E-817.6956523
GCCGCTC1156.4030246E-817.69565227
Produced by FastQC (version 0.11.5)