FastQCFastQC Report
Fri 10 Feb 2017
ERR1633383.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633383.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067102
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31560.29575429527823954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20590.1929525012604231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11490.10767480522011955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7850.027.8089181
GTATCAA21000.023.1690481
TTAACGG2050.022.56097635
TAACGGC2250.020.55555536
AACGGCC2350.019.68085137
TCGATAC1800.019.5277791
CTCTAAT2050.018.0487821
GTCGATT2100.017.6190512
TAATACT2850.016.8771934
TTTAACG2850.016.87719334
CGAGTCG1554.0017767E-1016.70967921
CGTAGGC904.4454755E-516.44444528
CCGCTCT1708.54925E-1116.3235328
GGTCGAT2200.015.97727311
ATTAGAG3250.015.9384623
CTTAGAC1059.341762E-615.8571443
TATTAGA3650.015.7123292
CTAACCG1659.731593E-1015.696976
ATCAACG31550.015.4215533
CGATTGG2400.015.41666714
Produced by FastQC (version 0.11.5)