##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633383.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1067102
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42378610479598	33.0	33.0	33.0	27.0	33.0
2	31.634649733577483	33.0	33.0	33.0	27.0	33.0
3	31.376204898875645	33.0	33.0	33.0	27.0	33.0
4	31.682381815421582	33.0	33.0	33.0	27.0	33.0
5	31.790303082554434	33.0	33.0	33.0	27.0	33.0
6	34.81987007802441	37.0	37.0	37.0	27.0	37.0
7	34.98858403414106	37.0	37.0	37.0	33.0	37.0
8	35.11835044822332	37.0	37.0	37.0	33.0	37.0
9	35.26360272963597	37.0	37.0	37.0	33.0	37.0
10	35.275787131876804	37.0	37.0	37.0	33.0	37.0
11	35.26885808479415	37.0	37.0	37.0	33.0	37.0
12	35.28827234884763	37.0	37.0	37.0	33.0	37.0
13	35.28514893609046	37.0	37.0	37.0	33.0	37.0
14	35.27463635153903	37.0	37.0	37.0	33.0	37.0
15	35.28166004749312	37.0	37.0	37.0	33.0	37.0
16	35.26690981743076	37.0	37.0	37.0	33.0	37.0
17	35.25049432950177	37.0	37.0	37.0	33.0	37.0
18	35.231898169059754	37.0	37.0	37.0	33.0	37.0
19	35.22294682232814	37.0	37.0	37.0	33.0	37.0
20	35.22632138258573	37.0	37.0	37.0	33.0	37.0
21	35.230334119887324	37.0	37.0	37.0	33.0	37.0
22	35.12543037122974	37.0	37.0	37.0	33.0	37.0
23	35.17200605003083	37.0	37.0	37.0	33.0	37.0
24	35.16253085459497	37.0	37.0	37.0	33.0	37.0
25	35.19190761520454	37.0	37.0	37.0	33.0	37.0
26	35.072710012726056	37.0	37.0	37.0	33.0	37.0
27	35.07234641112096	37.0	37.0	37.0	27.0	37.0
28	35.08949003937768	37.0	37.0	37.0	33.0	37.0
29	35.09479599888296	37.0	37.0	37.0	33.0	37.0
30	35.10236884571484	37.0	37.0	37.0	33.0	37.0
31	35.101074686393616	37.0	37.0	37.0	33.0	37.0
32	35.09369769712736	37.0	37.0	37.0	33.0	37.0
33	35.07910583992908	37.0	37.0	37.0	33.0	37.0
34	35.067288787763495	37.0	37.0	37.0	33.0	37.0
35	35.00677535980628	37.0	37.0	37.0	27.0	37.0
36	35.04832902571638	37.0	37.0	37.0	27.0	37.0
37	35.05412416057696	37.0	37.0	37.0	27.0	37.0
38	35.037000211788566	37.0	37.0	37.0	27.0	37.0
39	34.93386855239705	37.0	37.0	37.0	27.0	37.0
40	34.732050919218594	37.0	37.0	37.0	27.0	37.0
41	34.922843364551845	37.0	37.0	37.0	27.0	37.0
42	34.96365858184129	37.0	37.0	37.0	27.0	37.0
43	34.585133379939315	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	29.0
16	59.0
17	49.0
18	35.0
19	57.0
20	111.0
21	319.0
22	899.0
23	1926.0
24	3637.0
25	6204.0
26	9635.0
27	14376.0
28	19641.0
29	25471.0
30	32743.0
31	40811.0
32	50801.0
33	66523.0
34	95240.0
35	184840.0
36	513691.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.601177769322895	19.700084902849024	13.68266576203587	25.01607156579221
2	16.496361172596437	21.251951547274768	37.949511855473986	24.302175424654813
3	18.543494436333173	26.353432005562734	29.240691142927293	25.862382415176803
4	13.254965317279884	17.94298951740321	37.81063103620835	30.99141412910856
5	14.912070261324597	36.1342214708622	34.58132399714366	14.372384270669533
6	30.596231662952555	38.55423380332902	17.205384302531527	13.644150231186897
7	27.687231398685412	31.385659477725653	22.67224688923833	18.254862234350604
8	25.15279701471837	35.160931194956056	20.12619224778887	19.560079542536702
9	26.2733084559864	15.73073614331151	19.632706151801795	38.363249248900296
10	15.52082181459692	27.67982817012807	32.91559757174103	23.883752443533982
11	34.638769302278504	23.331321654349818	23.613862592329504	18.416046451042167
12	23.964719398895326	24.999953144123054	29.403281035927215	21.632046421054408
13	28.62781627248379	21.741126902582884	25.481631559119933	24.14942526581339
14	22.681430641119594	20.515752008711445	26.754237176952156	30.048580173216806
15	24.3915764378663	27.947843786254733	23.68274073143898	23.97783904443999
16	23.63953961289549	27.726496623565506	24.507591589182663	24.12637217435634
17	23.271346131859936	26.780570179795372	25.71712919664662	24.230954491698075
18	24.276404692334943	26.373392609141394	26.627632597446166	22.722570101077498
19	25.620606090139464	25.868848526195247	26.152795140483292	22.357750243182
20	25.15635806136621	25.66427576745241	26.3605540988584	22.818812072322984
21	24.161232946803583	26.15729330467003	26.502714829510204	23.178758919016175
22	23.75845982858246	25.968276697073005	26.517521286624895	23.755742187719637
23	23.72612927349026	26.008197904230336	26.939505314393564	23.326167507885845
24	23.849922500379535	26.471227680203018	25.74271250545871	23.936137313958742
25	24.02066531596792	26.079699972448743	26.7322149147879	23.167419796795432
26	24.099570612743673	26.286896660300513	26.344248253681467	23.269284473274347
27	23.834553772741497	26.16047950430231	26.12261995573057	23.882346767225627
28	23.540673712541068	26.14295540632479	26.5821823968093	23.734188484324836
29	24.285869579477875	26.16357199218069	25.98083407209433	23.569724356247107
30	24.16470028169753	25.897243187624053	26.391666401150033	23.546390129528387
31	24.213242970212782	25.911018815445946	26.187374777668865	23.688363436672407
32	23.329166284010338	25.798752134285195	26.65171651819601	24.220365063508456
33	23.395233070503103	26.065080938841835	26.969680499146286	23.570005491508777
34	23.899496018187577	25.6320389241141	26.590522742905552	23.877942314792776
35	23.652096987916806	26.333096554968506	26.408440805096422	23.606365652018273
36	24.49334740259132	25.92882404868513	26.114373321388207	23.463455227335345
37	24.106692706039347	25.638786170394205	26.26852915653799	23.985991967028454
38	23.918425792473446	26.185219407329384	26.130866590072927	23.765488210124243
39	23.69979627064704	25.863975514992944	26.460075981490057	23.97615223286996
40	23.719382027210145	26.21829965645271	26.660619134815605	23.40169918152154
41	23.815811421963414	25.587057282246683	26.634660978987952	23.962470316801955
42	23.459425621918054	26.25672147554779	26.536919619680216	23.746933282853934
43	23.888531742982394	25.583777370860517	26.571124409850228	23.956566476306858
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	22.0
2	35.0
3	148.0
4	261.0
5	261.0
6	304.5
7	348.0
8	366.0
9	384.0
10	495.5
11	607.0
12	607.0
13	1012.5
14	1418.0
15	2632.0
16	3846.0
17	4540.0
18	5234.0
19	5234.0
20	4913.5
21	4593.0
22	5316.5
23	6040.0
24	8148.0
25	10256.0
26	10256.0
27	13682.0
28	17108.0
29	21373.0
30	25638.0
31	30415.5
32	35193.0
33	35193.0
34	42176.5
35	49160.0
36	53982.5
37	58805.0
38	64164.5
39	69524.0
40	69524.0
41	74242.5
42	78961.0
43	81373.5
44	83786.0
45	84084.0
46	84382.0
47	84382.0
48	83149.5
49	81917.0
50	82255.0
51	82593.0
52	79906.0
53	77219.0
54	77219.0
55	73773.0
56	70327.0
57	63251.0
58	56175.0
59	52512.0
60	48849.0
61	48849.0
62	43074.5
63	37300.0
64	31821.5
65	26343.0
66	22484.0
67	18625.0
68	18625.0
69	15806.0
70	12987.0
71	10441.5
72	7896.0
73	6340.0
74	4784.0
75	4784.0
76	3742.0
77	2700.0
78	2137.5
79	1575.0
80	1249.0
81	923.0
82	923.0
83	760.5
84	598.0
85	495.0
86	392.0
87	290.5
88	189.0
89	189.0
90	143.5
91	98.0
92	59.5
93	21.0
94	11.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1067102.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.50732881485749
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.15578459194192	50.07885945385004
2	9.016098187249922	10.730478389114431
3	2.5930937032888464	4.629242389480376
4	1.1790655663446403	2.806521694029867
5	0.6731755409090179	2.0029439131496236
6	0.42188620211699324	1.5063192571096407
7	0.3054564211509452	1.2723826984427216
8	0.21984638581638558	1.0465976935626942
9	0.17939188835537873	0.9607618878373553
>10	1.082459483169237	12.356205158407336
>50	0.10903984715048007	4.522068352827194
>100	0.061833613373624564	6.597779707463131
>500	0.0023904742771596508	0.8863169367641899
>1k	4.780948554319302E-4	0.6035224679614063
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3156	0.29575429527823954	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2059	0.1929525012604231	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1149	0.10767480522011955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.811352616713304E-4	0.0	0.0
2	0.0	0.0	2.811352616713304E-4	0.0	0.0
3	0.0	0.0	4.685587694522173E-4	0.0	0.0
4	0.0	0.0	5.622705233426608E-4	0.0	0.0
5	9.371175389044346E-5	0.0	6.559822772331043E-4	0.0	0.0
6	9.371175389044346E-5	0.0	0.0021553703394802	0.0	0.0
7	9.371175389044346E-5	0.0	0.0035610466478368514	1.8742350778088693E-4	0.0
8	9.371175389044346E-5	0.0	0.004779299448412617	1.8742350778088693E-4	0.0
9	1.8742350778088693E-4	0.0	0.005903840495097938	8.434057850139912E-4	0.0
10	3.7484701556177386E-4	0.0	0.006559822772331043	9.371175389044346E-4	9.371175389044346E-5
11	3.7484701556177386E-4	0.0	0.009371175389044346	0.001030829292794878	9.371175389044346E-5
12	3.7484701556177386E-4	0.0	0.010402004681839225	0.001030829292794878	9.371175389044346E-5
13	3.7484701556177386E-4	0.0	0.011057986959072329	0.001030829292794878	9.371175389044346E-5
14	3.7484701556177386E-4	0.0	0.011995104497976763	0.0011245410466853216	9.371175389044346E-5
15	3.7484701556177386E-4	0.0	0.013307069052442971	0.0011245410466853216	1.8742350778088693E-4
16	3.7484701556177386E-4	0.0	0.013869339575785632	0.0011245410466853216	1.8742350778088693E-4
17	3.7484701556177386E-4	0.0	0.014244186591347406	0.0011245410466853216	1.8742350778088693E-4
18	3.7484701556177386E-4	0.0	0.014900168868580511	0.0013119645544662085	1.8742350778088693E-4
19	3.7484701556177386E-4	0.0	0.015275015884142285	0.0013119645544662085	1.8742350778088693E-4
20	3.7484701556177386E-4	0.0	0.015368727638032728	0.0014993880622470954	1.8742350778088693E-4
21	3.7484701556177386E-4	0.0	0.015837286407484944	0.0014993880622470954	1.8742350778088693E-4
22	3.7484701556177386E-4	0.0	0.015930998161375387	0.0021553703394802	1.8742350778088693E-4
23	3.7484701556177386E-4	0.0	0.016024709915265833	0.00243650560115153	1.8742350778088693E-4
24	3.7484701556177386E-4	0.0	0.01621213342304672	0.0027176408628228604	1.8742350778088693E-4
25	3.7484701556177386E-4	0.0	0.01621213342304672	0.0028113526167133037	1.8742350778088693E-4
26	3.7484701556177386E-4	0.0	0.01621213342304672	0.002998776124494191	1.8742350778088693E-4
27	3.7484701556177386E-4	0.0	0.01621213342304672	0.003935893663398625	1.8742350778088693E-4
28	3.7484701556177386E-4	0.0	0.01621213342304672	0.010495716435729668	1.8742350778088693E-4
29	3.7484701556177386E-4	0.0	0.01621213342304672	0.023709073734282195	1.8742350778088693E-4
30	3.7484701556177386E-4	0.0	0.01621213342304672	0.04245142451237089	1.8742350778088693E-4
31	3.7484701556177386E-4	0.0	0.01621213342304672	0.0989596121083083	1.8742350778088693E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	785	0.0	27.808918	1
GTATCAA	2100	0.0	23.169048	1
TTAACGG	205	0.0	22.560976	35
TAACGGC	225	0.0	20.555555	36
AACGGCC	235	0.0	19.680851	37
TCGATAC	180	0.0	19.527779	1
CTCTAAT	205	0.0	18.048782	1
GTCGATT	210	0.0	17.61905	12
TAATACT	285	0.0	16.877193	4
TTTAACG	285	0.0	16.877193	34
CGAGTCG	155	4.0017767E-10	16.709679	21
CGTAGGC	90	4.4454755E-5	16.444445	28
CCGCTCT	170	8.54925E-11	16.32353	28
GGTCGAT	220	0.0	15.977273	11
ATTAGAG	325	0.0	15.938462	3
CTTAGAC	105	9.341762E-6	15.857144	3
TATTAGA	365	0.0	15.712329	2
CTAACCG	165	9.731593E-10	15.69697	6
ATCAACG	3155	0.0	15.421553	3
CGATTGG	240	0.0	15.416667	14
>>END_MODULE