FastQCFastQC Report
Fri 10 Feb 2017
ERR1633382.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633382.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721716
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160552.2245592449107408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT119381.6541132523042303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66160.9167040775041706No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28830.3994646093477212No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG27190.37674098953050783No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA23650.3276912248031082No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19770.27393046572335933No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT19750.2736533484085153No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG18760.259936041323734No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC17370.24067638794207138No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG16280.22557349428306978No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.21684429886548173No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15190.2104706006240682No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14990.20769942747562753No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13430.1860842769177904No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12430.17222841117558707No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA12300.17042714862910063No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12190.16890300339745826No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC11670.16169795321151256No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11290.1564327242294753No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC11240.15573993094236513No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC11080.15352299242361261No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10870.1506132606177499No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT10800.14964335001579568No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10790.14950479135837363No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC10760.14908911538610756No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10640.14742641149704314No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10540.14604082492282283No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10150.14063703728336355No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG10140.1404984786259415No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC9920.13745018816265678No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9760.13523324964390426No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9710.1345404563567941No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT9630.13343198709741783No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9620.13329342843999578No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9550.13232351783804155No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9300.12885955140249072No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA9290.12872099274506868No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9260.1283053167728026No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.12775108214311445No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9130.12650405422631616No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9030.12511846765209583No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8850.12262441181849924No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8780.12165450121654502No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8700.12054603195716874No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8570.11874476941068231No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT8320.11528080297513149No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT8320.11528080297513149No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8290.11486512700286539No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8250.11431089237317726No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC8150.11292530579895693No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8110.11237107116926881No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT8020.1111240432524705No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.11056980862278237No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC7920.10973845667825016No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7920.10973845667825016No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7810.10821431144660781No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7770.10766007681691966No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7700.10669016621496544No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC7650.10599737292785527No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7610.10544313829816715No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7380.1022562891774604No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7360.10197917186261633No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC7360.10197917186261633No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG7350.10184061320519429No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7330.10156349589035023No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC7330.10156349589035023No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7260.100593585288396No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7220.10003935065870785No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7220.10003935065870785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCC303.598164E-430.83333215
GTTATGC358.866183E-426.4285721
GGTATCA27300.025.2765561
GCCGGCA7100.025.27464715
CCGGCAG7150.024.8391616
GGCAGCT7150.024.5804218
CAGCTTC7500.024.41999820
GCAGCTT7400.024.2519
CGCTCTC7250.023.98620829
GTACTAT701.9208528E-723.7857151
CGCCGGC7700.023.78571314
CCGCTCT7350.023.65986428
TTCCCTG551.901387E-523.54545436
GCCGCTC7400.023.527
GCTCTCT7450.023.34228130
CGAGCGG400.001930494423.12500235
GACTCCG400.001930494423.12500220
CGGCAGC7850.023.09554117
TATTAGC1054.0017767E-1122.9047642
TTGCGGT1054.0017767E-1122.90476435
Produced by FastQC (version 0.11.5)