FastQCFastQC Report
Fri 10 Feb 2017
ERR1633381.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633381.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542963
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32330.5954365214572632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22590.416050449109792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12890.2374010752113864No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8250.15194405511977796No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7460.13739426075073258No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.11437243421743286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG250.005493821629.5999985
GGTATCA5950.023.3193261
GCCGGCA502.6995587E-422.19999915
TAAGGTA502.6995587E-422.1999995
GTATCAA13800.021.5833321
ACCGAGC555.138291E-420.18181827
TGCTCGC656.893558E-519.92307910
ACGTGGG951.6720514E-719.47368436
GCGACAA609.228263E-418.516
TTAGCGT609.228263E-418.525
TAGCGTT609.228263E-418.526
CCTACCG609.228263E-418.524
CGAGCCT609.228263E-418.529
GTATAGG1501.2732926E-1118.51
TATACTG609.228263E-418.55
GGCGACA609.228263E-418.515
TACCGAG609.228263E-418.526
TAAGGAG500.007030219318.4999985
GCCACGT1054.785743E-717.61904733
TAGACAG953.5993016E-617.5263165
Produced by FastQC (version 0.11.5)