##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633381.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	542963
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.545764628529017	33.0	33.0	33.0	27.0	33.0
2	31.734501245941253	33.0	33.0	33.0	27.0	33.0
3	31.465018795019184	33.0	33.0	33.0	27.0	33.0
4	31.766096400675554	33.0	33.0	33.0	27.0	33.0
5	31.869088685601046	33.0	33.0	33.0	33.0	33.0
6	34.93165280138794	37.0	37.0	37.0	27.0	37.0
7	35.087657906708195	37.0	37.0	37.0	33.0	37.0
8	35.221707556500164	37.0	37.0	37.0	33.0	37.0
9	35.35218974405254	37.0	37.0	37.0	33.0	37.0
10	35.366115186486006	37.0	37.0	37.0	33.0	37.0
11	35.37554492663404	37.0	37.0	37.0	33.0	37.0
12	35.36228987978923	37.0	37.0	37.0	33.0	37.0
13	35.37114131165475	37.0	37.0	37.0	33.0	37.0
14	35.36330836539506	37.0	37.0	37.0	33.0	37.0
15	35.36599915648028	37.0	37.0	37.0	33.0	37.0
16	35.34969786154858	37.0	37.0	37.0	33.0	37.0
17	35.33928831246328	37.0	37.0	37.0	33.0	37.0
18	35.3218267174743	37.0	37.0	37.0	33.0	37.0
19	35.30409622755142	37.0	37.0	37.0	33.0	37.0
20	35.30404465865998	37.0	37.0	37.0	33.0	37.0
21	35.29796321296295	37.0	37.0	37.0	33.0	37.0
22	35.20161226455578	37.0	37.0	37.0	33.0	37.0
23	35.2416684009776	37.0	37.0	37.0	33.0	37.0
24	35.23284827879616	37.0	37.0	37.0	33.0	37.0
25	35.256352642813596	37.0	37.0	37.0	33.0	37.0
26	35.11989030560093	37.0	37.0	37.0	33.0	37.0
27	35.1523842324431	37.0	37.0	37.0	33.0	37.0
28	35.157802649535974	37.0	37.0	37.0	33.0	37.0
29	35.153457970432605	37.0	37.0	37.0	33.0	37.0
30	35.16703348110276	37.0	37.0	37.0	33.0	37.0
31	35.15270469626844	37.0	37.0	37.0	33.0	37.0
32	35.14713709773962	37.0	37.0	37.0	33.0	37.0
33	35.13795967681039	37.0	37.0	37.0	33.0	37.0
34	35.10245449505767	37.0	37.0	37.0	33.0	37.0
35	35.04173028364732	37.0	37.0	37.0	27.0	37.0
36	35.05567598528813	37.0	37.0	37.0	27.0	37.0
37	35.05399447107814	37.0	37.0	37.0	27.0	37.0
38	35.02651377718187	37.0	37.0	37.0	27.0	37.0
39	34.92146242009124	37.0	37.0	37.0	27.0	37.0
40	34.71012941950004	37.0	37.0	37.0	27.0	37.0
41	34.88141549240004	37.0	37.0	37.0	27.0	37.0
42	34.908715695176284	37.0	37.0	37.0	27.0	37.0
43	34.54319355094178	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	19.0
16	29.0
17	28.0
18	22.0
19	25.0
20	63.0
21	155.0
22	406.0
23	915.0
24	1823.0
25	3100.0
26	4759.0
27	6956.0
28	9590.0
29	12537.0
30	15978.0
31	20050.0
32	25162.0
33	32722.0
34	46890.0
35	92465.0
36	269266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.59337376579988	21.62099443240147	14.12214091936283	23.66349088243582
2	15.688545996688541	22.65752915023676	39.354983672920625	22.29894118015408
3	17.747618161826864	27.888824837051512	31.101014249589753	23.26254275153187
4	12.369903658260323	18.547120153675298	40.17142972909756	28.911546458966818
5	13.44677261618195	37.873667266461986	35.35434274526993	13.325217372086126
6	27.75603494160744	40.98161384845744	17.90379823302877	13.358552976906346
7	24.975182470997105	33.53414505224113	23.70953453550242	17.78113794125935
8	23.7227951075856	36.058073938739845	21.341233196368815	18.877897757305746
9	25.498422544445937	15.515237686545861	20.900871698439857	38.085468070568346
10	14.55568795663793	28.52901578928951	34.19754200562469	22.717754248447868
11	32.79118466635848	24.553422608907052	24.664664074715954	17.990728650018507
12	22.039070802246194	26.48817691076556	30.970986973329673	20.501765313658574
13	27.226717105953814	23.175796509154402	27.132419704473413	22.46506668041837
14	21.528354602431474	22.06780204175975	28.733265434292942	27.670577921515832
15	23.545250781360792	29.185598282019214	25.663811346261163	21.605339590358827
16	21.838504649488087	29.42668284947593	25.856642165304084	22.8781703357319
17	21.67569429224459	28.21518224998757	26.98010730012911	23.129016157638734
18	21.902965763781328	27.61937737930577	28.93180566631612	21.545851190596782
19	24.03975961529607	26.863340595952213	28.833456423365867	20.263443365385854
20	23.96903656418577	26.15924105325777	28.889998029331647	20.981724353224806
21	22.1250803461746	27.841307787086784	28.34244690706365	21.691164959674968
22	21.57955514464153	27.79121229255032	28.374309114985735	22.25492344782241
23	21.716028532330935	27.546996756685076	28.94874973064463	21.78822498033936
24	22.514425476505764	27.65768569865718	27.837255945616917	21.990632879220133
25	22.470039394949566	27.41254928973061	28.59402942741955	21.523381887900282
26	22.35106259542547	27.55031189970587	28.2391249495822	21.859500555286456
27	22.377399564979566	27.255632520079637	28.308006254569833	22.058961660370965
28	21.431110407154815	27.756219116219704	28.753340467029982	22.0593300095955
29	22.86988247817991	27.59359293358848	27.74608951254505	21.790435075686556
30	22.16246779246468	27.52968434313204	28.694772940329266	21.613074924074017
31	22.55089204973451	27.27147153673455	28.27761744354588	21.900018969985062
32	21.08836145372705	27.307201411514225	28.72995029127215	22.874486843486572
33	21.417849835071635	27.212351486197033	29.30254916080838	22.067249517922953
34	21.581028541539663	27.333538381068323	28.6266283337907	22.458804743601313
35	21.94514174999033	28.0089066842492	28.035427828415564	22.010523737344904
36	22.475564633317553	27.63595309440975	28.10965019715892	21.778832075113776
37	22.68699708819938	26.925591614898252	27.88845648782698	22.49895480907539
38	22.539473223773996	27.41899540115993	27.991041746859363	22.05048962820671
39	22.112924821765017	27.22984807436234	28.505809788143942	22.151417315728697
40	22.084930280700526	27.786423752631396	28.343736129349512	21.784909837318565
41	22.101321821192236	27.04696268438181	28.585004871418494	22.26671062300746
42	21.904070811454922	27.45435692671508	28.43121907017605	22.210353191653944
43	21.957297274399913	27.16188764243604	28.237651552684067	22.643163530479978
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	39.0
2	48.0
3	171.0
4	294.0
5	294.0
6	367.0
7	440.0
8	437.5
9	435.0
10	605.5
11	776.0
12	776.0
13	1250.0
14	1724.0
15	3295.0
16	4866.0
17	5571.5
18	6277.0
19	6277.0
20	6009.0
21	5741.0
22	6570.5
23	7400.0
24	9121.0
25	10842.0
26	10842.0
27	12800.5
28	14759.0
29	17781.0
30	20803.0
31	22658.5
32	24514.0
33	24514.0
34	27245.5
35	29977.0
36	31526.0
37	33075.0
38	35081.0
39	37087.0
40	37087.0
41	38838.5
42	40590.0
43	39828.0
44	39066.0
45	39243.0
46	39420.0
47	39420.0
48	39033.0
49	38646.0
50	39169.5
51	39693.0
52	39191.5
53	38690.0
54	38690.0
55	35062.0
56	31434.0
57	27648.5
58	23863.0
59	20788.0
60	17713.0
61	17713.0
62	15266.5
63	12820.0
64	10422.5
65	8025.0
66	6655.5
67	5286.0
68	5286.0
69	4305.0
70	3324.0
71	2821.0
72	2318.0
73	1814.0
74	1310.0
75	1310.0
76	1002.5
77	695.0
78	534.5
79	374.0
80	285.5
81	197.0
82	197.0
83	177.5
84	158.0
85	142.5
86	127.0
87	103.0
88	79.0
89	79.0
90	56.5
91	34.0
92	23.0
93	12.0
94	6.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	542963.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.177123311697855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.92865118886071	51.736131061573154
2	6.373641590767276	7.416002655412678
3	1.5043411262659991	2.625547176169064
4	0.6899256002325449	1.6055154688250368
5	0.4176869119665867	1.2149911491581198
6	0.28333427851511384	0.9890143955762832
7	0.20760489359720716	0.8454498846441642
8	0.14792939278677097	0.688488522046445
9	0.11915414502197183	0.6238840849238865
>10	1.045191399357597	12.69829275910877
>50	0.19018317610859295	7.671442198001639
>100	0.08978190497908914	10.012919754803331
>500	0.0016089947128869022	0.6028270437975232
>1k	9.653968277321413E-4	1.2694938459599392
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3233	0.5954365214572632	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2259	0.416050449109792	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1289	0.2374010752113864	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	825	0.15194405511977796	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	746	0.13739426075073258	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	621	0.11437243421743286	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.8417461226639752E-4	0.0	0.0
2	0.0	0.0	3.6834922453279505E-4	0.0	0.0
3	0.0	0.0	3.6834922453279505E-4	0.0	0.0
4	0.0	0.0	3.6834922453279505E-4	0.0	0.0
5	0.0	0.0	5.525238367991926E-4	0.0	0.0
6	0.0	0.0	7.366984490655901E-4	0.0	0.0
7	0.0	0.0	0.002578444571729565	0.0	0.0
8	0.0	0.0	0.002762619183995963	0.0	0.0
9	0.0	0.0	0.0031309684085287577	3.6834922453279505E-4	0.0
10	0.0	0.0	0.0036834922453279507	3.6834922453279505E-4	0.0
11	1.8417461226639752E-4	0.0	0.005709412980258323	3.6834922453279505E-4	0.0
12	1.8417461226639752E-4	0.0	0.006446111429323913	5.525238367991926E-4	0.0
13	1.8417461226639752E-4	0.0	0.006998635266123106	5.525238367991926E-4	0.0
14	1.8417461226639752E-4	0.0	0.00810368293972149	5.525238367991926E-4	0.0
15	1.8417461226639752E-4	0.0	0.009024556001053478	5.525238367991926E-4	0.0
16	1.8417461226639752E-4	0.0	0.010682127511451056	9.208730613319877E-4	0.0
17	1.8417461226639752E-4	0.0	0.011050476735983852	0.0011050476735983852	0.0
18	1.8417461226639752E-4	0.0	0.011050476735983852	0.0012892222858647826	0.0
19	1.8417461226639752E-4	0.0	0.011050476735983852	0.0014733968981311802	0.0
20	1.8417461226639752E-4	0.0	0.011050476735983852	0.0016575715103975778	0.0
21	1.8417461226639752E-4	0.0	0.011603000572783044	0.0016575715103975778	0.0
22	1.8417461226639752E-4	0.0	0.011603000572783044	0.0016575715103975778	0.0
23	1.8417461226639752E-4	0.0	0.011603000572783044	0.0016575715103975778	0.0
24	1.8417461226639752E-4	0.0	0.011603000572783044	0.0022100953471967705	0.0
25	1.8417461226639752E-4	0.0	0.011787175185049441	0.002578444571729565	0.0
26	1.8417461226639752E-4	0.0	0.011787175185049441	0.002762619183995963	0.0
27	1.8417461226639752E-4	0.0	0.011787175185049441	0.003867666857594348	0.0
28	1.8417461226639752E-4	0.0	0.011787175185049441	0.012523873634115031	0.0
29	1.8417461226639752E-4	0.0	0.011787175185049441	0.03149385869755398	0.0
30	1.8417461226639752E-4	0.0	0.011787175185049441	0.051753066046857706	0.0
31	1.8417461226639752E-4	0.0	0.011787175185049441	0.10129603674651864	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	25	0.0054938216	29.599998	5
GGTATCA	595	0.0	23.319326	1
GCCGGCA	50	2.6995587E-4	22.199999	15
TAAGGTA	50	2.6995587E-4	22.199999	5
GTATCAA	1380	0.0	21.583332	1
ACCGAGC	55	5.138291E-4	20.181818	27
TGCTCGC	65	6.893558E-5	19.923079	10
ACGTGGG	95	1.6720514E-7	19.473684	36
GCGACAA	60	9.228263E-4	18.5	16
TTAGCGT	60	9.228263E-4	18.5	25
TAGCGTT	60	9.228263E-4	18.5	26
CCTACCG	60	9.228263E-4	18.5	24
CGAGCCT	60	9.228263E-4	18.5	29
GTATAGG	150	1.2732926E-11	18.5	1
TATACTG	60	9.228263E-4	18.5	5
GGCGACA	60	9.228263E-4	18.5	15
TACCGAG	60	9.228263E-4	18.5	26
TAAGGAG	50	0.0070302193	18.499998	5
GCCACGT	105	4.785743E-7	17.619047	33
TAGACAG	95	3.5993016E-6	17.526316	5
>>END_MODULE