FastQCFastQC Report
Fri 10 Feb 2017
ERR1633380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562420
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT174453.101774474591942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130192.3148181074641725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73571.3080971515948936No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33140.5892393584865403No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG30370.5399879093915579No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA29950.5325201806479144No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA24630.4379289498950962No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG22750.40450197361402507No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT18870.3355143842679848No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18770.33373635361473636No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG18340.3260908218057679No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA17730.31524483482095234No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17220.3061768784893852No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC16620.2955086945698944No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15810.28110664627858184No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT13820.24572383627893746No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13110.2330998186408734No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12970.23061057572632554No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12220.21727534582696206No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC12150.21603072436968815No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11900.21158564773656696No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC11850.21069663240994274No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11530.20500693431954767No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11530.20500693431954767No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11490.2042957220582483No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11250.20002844849045198No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC11230.19967284235980226No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA11190.1989616300985029No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC11080.1970057963799296No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.1946943565307066No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC10350.18402617261121582No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10330.18367056648056612No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA10310.18331496034991643No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10200.18135912663134313No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10050.17869208065147044No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA9920.1763806408022474No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT9800.17424700401834928No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA9700.1724689733651008No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9650.1715799580384766No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT9560.16997973045055295No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA9540.16962412431990329No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT9520.16926851818925356No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG9450.16802389673197968No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9420.16749048753600512No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.16073397105366097No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC9000.16002275879236158No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8770.15593328828989012No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8720.1550442729632659No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8480.1507769993954696No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8470.15059919633014474No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8440.1500657871341702No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8400.14935457487287082No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8180.1454429074357242No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8180.1454429074357242No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7870.13993101241065398No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7820.13904199708402973No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7800.13868639095338003No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7770.1381529817574055No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7730.13744176949610612No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7710.13708616336545643No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA7700.13690836030013157No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG7570.13459692045090857No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7470.13281888979766013No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC7440.13228548060168557No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC7410.13175207140571102No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG7280.12944063155648802No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7250.1289072223605135No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7190.12784040396856441No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7150.12712919170726503No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA7060.12552896411934142No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7010.12463994879271718No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6970.1239287365314178No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG6960.12375093346609295No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6660.11841684150634757No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC6480.11521638633050033No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.11521638633050033No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT6390.11361615874257672No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT6310.11219373421997796No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA6310.11219373421997796No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC6270.11148252195867857No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC6240.11094911276270403No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC5950.10579282386828348No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5880.10454820241100955No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5870.10437039934568472No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG5810.10330358095373565No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG5760.1024145656271114No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5760.1024145656271114No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA5740.10205895949646172No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5740.10205895949646172No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5690.10116994416983749No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT5680.10099214110451263No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5660.10063653497386295No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG5650.10045873190853812No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5630.10010312577788841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAAC355.624479E-737.02
CTAGTCG352.386156E-531.7142859
TGCTCTC352.386156E-531.71428517
ACTTTAT250.00549398629.622
GGTCGCT405.9348175E-527.75000228
TTGCTCT405.9348175E-527.75000216
CAGCTAG405.9348175E-527.7500029
AAGCAAG509.080311E-625.90000232
CTTCTCT509.080311E-625.90000233
CCGCTCT7400.025.7528
GCCGCTC7400.025.7527
CGCTCTC7450.025.5771829
GCTCTCT7500.025.16000230
TCAGCAA601.3353783E-624.66666823
GACCGTT1800.024.6666667
CGCGAGG451.3220387E-424.66666623
GCTTCTC451.3220387E-424.66666632
CGAGGTC451.3220387E-424.66666625
TCTCGCG451.3220387E-424.66666620
AGCTAGG451.3220387E-424.66666610
Produced by FastQC (version 0.11.5)