FastQCFastQC Report
Fri 10 Feb 2017
ERR1633379.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633379.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274243
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10550.3846953249490416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.3099441006698439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5280.19252998253373832No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5150.1877896609940819No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC4050.14767924796621973No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3800.13856324500534198No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3710.13528148393942596No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT3640.13272900311038022No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG3570.13017652228133444No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA3560.12981188216289935No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3360.12251907979419711No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG3060.11157987624114381No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG2980.10866275529366291No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG2950.10756883493835759No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC2830.10319315351713626No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA2810.10246387328026604No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA2760.10064067268809049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTA250.005489137529.633
TATTGCG250.005489137529.611
CTTAGAC250.005489137529.63
GGTATCA1900.029.2105271
TGCGGTA451.3196257E-424.66666636
CGTTGTA400.001927458923.12500221
ATAATAC400.001927458923.1250023
TAGGACC652.6704947E-622.769234
TAATACT1005.293259E-1022.24
TAATGCT502.6948375E-422.24
TCTATAC502.6948375E-422.23
TTAGGCT603.712983E-521.5833324
CTTGCGG450.00381849420.55555534
TTTTCGG450.00381849420.55555526
CGGTCAC555.1293784E-420.18181830
TATACCA851.2382461E-619.5882345
GTATCAA5100.019.2254921
TCGCCTA500.007021561718.535
TTAGGAC1002.8551403E-718.53
CGAACCG500.007021561718.531
Produced by FastQC (version 0.11.5)