FastQCFastQC Report
Fri 10 Feb 2017
ERR1633378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706529
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34950.4946718393724815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24940.35299329539197966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13920.19701951370715143No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12130.17168438945888986No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11920.16871211231244576No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC10160.1438015990851048No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9800.13870626683405776No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9430.1334693975760372No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8530.12073106694841966No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7970.11280499455790208No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7550.10686044026501389No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7360.10417123713251684No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7090.10034973794423158No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT7070.10006666393028453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15550.024.0321521
GGTATCA4600.022.9239141
TGCGGTA806.952305E-720.812536
GGTTAGA555.140398E-420.1818181
TTAGCCG1202.382876E-1020.04166829
TTCGCCG1052.2557288E-819.3809524
GCCGGCA1153.0449883E-919.30434815
CCGGTGC1254.110916E-1019.24000233
CACCGCC2900.019.1379311
ATCAAGC2750.018.83636330
GCACCGC2950.018.81355910
TTAACGG2950.018.81355935
TAGCCGG1306.9485395E-1018.530
CGAATGT609.2320127E-418.515
TATATCG500.007032266418.4999985
CCGTCCG500.007032266418.49999828
AACGGCC2950.018.18644137
CGAGGGT3000.017.88333316
CGGCAGC1351.1496013E-917.81481617
TAACGGC3150.017.61904736
Produced by FastQC (version 0.11.5)