##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633378.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	706529
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83788492758259	33.0	33.0	33.0	27.0	33.0
2	31.961054677161165	33.0	33.0	33.0	33.0	33.0
3	31.692486791058823	33.0	33.0	33.0	27.0	33.0
4	31.968308448768557	33.0	33.0	33.0	33.0	33.0
5	32.07202535210869	33.0	33.0	33.0	33.0	33.0
6	35.22402335926763	37.0	37.0	37.0	33.0	37.0
7	35.36405441248696	37.0	37.0	37.0	33.0	37.0
8	35.492884226974404	37.0	37.0	37.0	33.0	37.0
9	35.597739087850606	37.0	37.0	37.0	33.0	37.0
10	35.5964157168354	37.0	37.0	37.0	33.0	37.0
11	35.62492551615008	37.0	37.0	37.0	33.0	37.0
12	35.61972686188394	37.0	37.0	37.0	33.0	37.0
13	35.618276107562465	37.0	37.0	37.0	33.0	37.0
14	35.60732397396285	37.0	37.0	37.0	33.0	37.0
15	35.621738102753035	37.0	37.0	37.0	33.0	37.0
16	35.59435918412408	37.0	37.0	37.0	33.0	37.0
17	35.5944327833677	37.0	37.0	37.0	33.0	37.0
18	35.58293714766131	37.0	37.0	37.0	33.0	37.0
19	35.578597623027505	37.0	37.0	37.0	33.0	37.0
20	35.579875702200475	37.0	37.0	37.0	33.0	37.0
21	35.5687395704918	37.0	37.0	37.0	33.0	37.0
22	35.47910135323532	37.0	37.0	37.0	33.0	37.0
23	35.53514434651656	37.0	37.0	37.0	33.0	37.0
24	35.50973986913488	37.0	37.0	37.0	33.0	37.0
25	35.54057795221428	37.0	37.0	37.0	33.0	37.0
26	35.433390561463156	37.0	37.0	37.0	33.0	37.0
27	35.447799028773055	37.0	37.0	37.0	33.0	37.0
28	35.46001933395515	37.0	37.0	37.0	33.0	37.0
29	35.46537792503917	37.0	37.0	37.0	33.0	37.0
30	35.45859264092486	37.0	37.0	37.0	33.0	37.0
31	35.45909085118941	37.0	37.0	37.0	33.0	37.0
32	35.44451961632148	37.0	37.0	37.0	33.0	37.0
33	35.44875015745992	37.0	37.0	37.0	33.0	37.0
34	35.41742943318675	37.0	37.0	37.0	33.0	37.0
35	35.35872837491455	37.0	37.0	37.0	33.0	37.0
36	35.38593178765486	37.0	37.0	37.0	33.0	37.0
37	35.383661534063	37.0	37.0	37.0	33.0	37.0
38	35.35882320470922	37.0	37.0	37.0	33.0	37.0
39	35.264757709874615	37.0	37.0	37.0	33.0	37.0
40	35.06936728711773	37.0	37.0	37.0	33.0	37.0
41	35.23254388708744	37.0	37.0	37.0	33.0	37.0
42	35.26786869328789	37.0	37.0	37.0	33.0	37.0
43	34.93765294842816	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	23.0
16	36.0
17	25.0
18	30.0
19	21.0
20	62.0
21	122.0
22	302.0
23	771.0
24	1657.0
25	2957.0
26	4698.0
27	7098.0
28	10051.0
29	13417.0
30	17399.0
31	22321.0
32	28303.0
33	37978.0
34	54279.0
35	109336.0
36	395642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.810214442719264	21.144921156810266	13.68662857434019	24.35823582613028
2	15.86445850064187	21.548018552670875	39.224716890601805	23.362806056085457
3	17.27756397826558	28.06098546556475	30.100109125032375	24.561341431137294
4	12.056688402033037	18.01143335942332	40.62550864861881	29.30636958992483
5	13.210498082881243	37.78358708559734	35.87722513867088	13.128689692850543
6	28.77376583268344	40.787568521603504	17.395464305074526	13.04320134063853
7	25.99327133068848	32.55110547479297	24.047986706844306	17.40763648767425
8	24.13121046694474	36.06405398787594	20.80848769123419	18.996247853945132
9	25.76794441558662	15.199942252901156	20.640341726949636	38.391771604562585
10	14.90172377920793	28.174356608150553	34.36051457194255	22.563405040698967
11	33.15858230872335	24.110687600933577	24.450376417670046	18.280353672673026
12	22.622992120634823	26.236856519689923	30.307460840248595	20.83269051942666
13	27.166188507478108	22.756036907189937	26.41561775949749	23.662156825834465
14	21.535987907078123	21.252489282110147	28.453042974881427	28.758479835930302
15	23.69315343036167	29.015086429573305	25.133009402303376	22.158750737761647
16	22.3220844438091	28.740221562030715	25.6547148100078	23.28297918415238
17	21.772779319744835	28.037773396421095	26.719214639455707	23.470232644378363
18	22.230934611318148	27.66199264290638	28.487861078596914	21.619211667178558
19	24.27487052902287	26.50676759198844	27.879676559631665	21.338685319357026
20	23.425082339153807	26.83768111429255	28.125950951765606	21.61128559478804
21	22.60841380891655	27.41048138151442	28.098068161391822	21.883036648177214
22	22.12548954112287	27.33957134102068	27.927940679009637	22.606998438846812
23	22.196965729644504	27.377503258889586	28.500033261196638	21.925497750269273
24	22.566660391859358	27.795462040482416	26.91128035791878	22.726597209739445
25	22.39497600240047	27.39123234856602	28.29112463890371	21.922667010129803
26	22.76396297958046	27.503895806116947	28.103021956635892	21.62911925766671
27	22.648893392910978	27.4121798255981	27.194354371865842	22.744572409625082
28	21.886291999337608	27.11933975816987	28.70398808824549	22.290380154247032
29	23.489764751340708	26.902080452465505	27.626325317149046	21.981829479044737
30	22.92814590766975	26.72487611973465	28.203513231587095	22.143464741008508
31	22.461923006698946	27.07489713798018	28.161335203508987	22.301844651811887
32	21.468333217744778	26.83329346707637	28.469461267690356	23.228912047488496
33	21.619070130171586	27.220255644141993	28.743759987205053	22.416914238481365
34	22.510753274104815	27.071075638791896	28.482341135324944	21.93582995177834
35	22.595817015295903	27.455490149731997	27.74266873688129	22.206024098090808
36	23.23471506477441	27.321879215148986	27.31763310493978	22.125772615136814
37	22.35053338221078	26.72487611973465	27.872741246289962	23.051849251764615
38	22.70635741774223	27.273615095771014	27.555556813662285	22.464470672824472
39	21.90653179133482	27.278427354008116	28.18072577346436	22.634315081192703
40	22.2044671910141	27.701764541865938	28.32410276152854	21.76966550559142
41	22.012684546564966	26.6310370841112	28.275414031129646	23.080864338194186
42	21.580430527267815	27.92114690267491	27.930063734114242	22.56835883594304
43	22.721501877488397	26.395661041514217	28.005219884817183	22.8776171961802
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	49.0
2	67.0
3	225.5
4	384.0
5	384.0
6	485.0
7	586.0
8	513.0
9	440.0
10	637.0
11	834.0
12	834.0
13	1367.5
14	1901.0
15	3338.0
16	4775.0
17	5571.0
18	6367.0
19	6367.0
20	6223.0
21	6079.0
22	6733.5
23	7388.0
24	9487.0
25	11586.0
26	11586.0
27	14313.5
28	17041.0
29	19663.5
30	22286.0
31	25885.0
32	29484.0
33	29484.0
34	34949.5
35	40415.0
36	43784.5
37	47154.0
38	49888.5
39	52623.0
40	52623.0
41	54640.5
42	56658.0
43	57165.5
44	57673.0
45	55801.0
46	53929.0
47	53929.0
48	50947.5
49	47966.0
50	47921.5
51	47877.0
52	45604.5
53	43332.0
54	43332.0
55	42496.0
56	41660.0
57	35674.5
58	29689.0
59	26843.0
60	23997.0
61	23997.0
62	21374.0
63	18751.0
64	15081.0
65	11411.0
66	9358.5
67	7306.0
68	7306.0
69	6214.5
70	5123.0
71	4357.0
72	3591.0
73	3550.5
74	3510.0
75	3510.0
76	3077.0
77	2644.0
78	1888.5
79	1133.0
80	743.5
81	354.0
82	354.0
83	272.5
84	191.0
85	175.0
86	159.0
87	123.0
88	87.0
89	87.0
90	64.0
91	41.0
92	22.0
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	706529.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.708077722546456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.16520932866891	48.55806255983519
2	7.353874828142421	8.19340460976072
3	1.8326268089416984	3.062763501268393
4	0.8526275192482295	1.8999296044264937
5	0.508208066121671	1.4155647223365537
6	0.3193488865904419	1.067418755687341
7	0.226753541771501	0.8842402750208623
8	0.18407810399662877	0.8203709859570548
9	0.14707435174871888	0.7373906476389692
>10	1.1473033219587936	12.747760670468608
>50	0.14871626346935493	5.814650652995196
>100	0.10593128783171082	10.784315452080403
>500	0.006701249351877534	2.4631526392353953
>1k	0.0015464421581255845	1.55097492328888
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3495	0.4946718393724815	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2494	0.35299329539197966	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1392	0.19701951370715143	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1213	0.17168438945888986	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1192	0.16871211231244576	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1016	0.1438015990851048	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	980	0.13870626683405776	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	943	0.1334693975760372	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	853	0.12073106694841966	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	797	0.11280499455790208	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	755	0.10686044026501389	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	736	0.10417123713251684	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	709	0.10034973794423158	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	707	0.10006666393028453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4153700697352835E-4	0.0	1.4153700697352835E-4	0.0	0.0
2	1.4153700697352835E-4	0.0	1.4153700697352835E-4	0.0	0.0
3	1.4153700697352835E-4	0.0	2.830740139470567E-4	0.0	0.0
4	1.4153700697352835E-4	0.0	4.24611020920585E-4	4.24611020920585E-4	0.0
5	1.4153700697352835E-4	0.0	5.661480278941134E-4	4.24611020920585E-4	0.0
6	1.4153700697352835E-4	0.0	0.0011322960557882268	5.661480278941134E-4	0.0
7	1.4153700697352835E-4	0.0	0.003113814153417623	7.076850348676416E-4	0.0
8	1.4153700697352835E-4	0.0	0.004104573202232321	7.076850348676416E-4	0.0
9	1.4153700697352835E-4	0.0	0.00509533225104702	8.4922204184117E-4	0.0
10	2.830740139470567E-4	0.0	0.00594455429288819	9.907590488146983E-4	0.0
11	2.830740139470567E-4	0.0	0.008633757425385228	0.0011322960557882268	0.0
12	2.830740139470567E-4	0.0	0.010049127495120512	0.0011322960557882268	0.0
13	2.830740139470567E-4	0.0	0.01103988654393521	0.001273833062761755	0.0
14	2.830740139470567E-4	0.0	0.011747571578802851	0.0015569070767088116	0.0
15	2.830740139470567E-4	0.0	0.012172182599723436	0.0015569070767088116	0.0
16	2.830740139470567E-4	0.0	0.012596793620644023	0.0015569070767088116	0.0
17	2.830740139470567E-4	0.0	0.01273833062761755	0.0015569070767088116	0.0
18	2.830740139470567E-4	0.0	0.013162941648538136	0.0015569070767088116	0.0
19	2.830740139470567E-4	0.0	0.01358755266945872	0.00169844408368234	0.0
20	2.830740139470567E-4	0.0	0.013870626683405777	0.00169844408368234	0.0
21	2.830740139470567E-4	0.0	0.014295237704326362	0.0022645921115764535	0.0
22	2.830740139470567E-4	0.0	0.01443677471129989	0.0026892031324970383	0.0
23	2.830740139470567E-4	0.0	0.014719848725246946	0.002972277146444095	0.0
24	2.830740139470567E-4	0.0	0.014861385732220475	0.004104573202232321	0.0
25	2.830740139470567E-4	0.0	0.014861385732220475	0.004812258237099964	0.0
26	2.830740139470567E-4	0.0	0.014861385732220475	0.00509533225104702	0.0
27	2.830740139470567E-4	0.0	0.014861385732220475	0.006369165313808775	0.0
28	2.830740139470567E-4	0.0	0.014861385732220475	0.017692125871691042	0.0
29	2.830740139470567E-4	0.0	0.014861385732220475	0.037790380861932066	0.0
30	2.830740139470567E-4	0.0	0.014861385732220475	0.06213474606137894	0.0
31	2.830740139470567E-4	0.0	0.014861385732220475	0.11775878980197557	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1555	0.0	24.032152	1
GGTATCA	460	0.0	22.923914	1
TGCGGTA	80	6.952305E-7	20.8125	36
GGTTAGA	55	5.140398E-4	20.181818	1
TTAGCCG	120	2.382876E-10	20.041668	29
TTCGCCG	105	2.2557288E-8	19.38095	24
GCCGGCA	115	3.0449883E-9	19.304348	15
CCGGTGC	125	4.110916E-10	19.240002	33
CACCGCC	290	0.0	19.13793	11
ATCAAGC	275	0.0	18.836363	30
GCACCGC	295	0.0	18.813559	10
TTAACGG	295	0.0	18.813559	35
TAGCCGG	130	6.9485395E-10	18.5	30
CGAATGT	60	9.2320127E-4	18.5	15
TATATCG	50	0.0070322664	18.499998	5
CCGTCCG	50	0.0070322664	18.499998	28
AACGGCC	295	0.0	18.186441	37
CGAGGGT	300	0.0	17.883333	16
CGGCAGC	135	1.1496013E-9	17.814816	17
TAACGGC	315	0.0	17.619047	36
>>END_MODULE