FastQCFastQC Report
Fri 10 Feb 2017
ERR1633377.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633377.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506158
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27560.5444940117512713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.39868973719668566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10280.2030986371844365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.10135175182452912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAC200.001840962837.022
GTATCAA12300.025.2682931
GCCGGCA451.321783E-424.66666815
GGTATCA3950.024.354431
TCGCCAT903.8162398E-922.61111313
TTAGCCG855.1662937E-821.76470629
TAGCCGG855.1662937E-821.76470630
CGTCCGC603.7209866E-521.58333229
GTCGCCA957.1213435E-921.42105312
CGAGTCG705.0926174E-621.14285921
AGCCGGT806.944283E-720.812531
CCGGCAG555.13763E-420.18181816
AGCTTCG555.13763E-420.18181821
GCTTCGC555.13763E-420.18181822
TGCTCGC555.13763E-420.18181810
GGACCGT555.13763E-420.1818186
CTAATGC555.13763E-420.1818183
CCGTCCG656.892381E-519.92307728
TGTCCGG851.2424935E-619.58823614
AGAGGTC1052.2519089E-819.380958
Produced by FastQC (version 0.11.5)