##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633377.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	506158
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.862339427609562	33.0	33.0	33.0	33.0	33.0
2	31.986225644956715	33.0	33.0	33.0	33.0	33.0
3	31.7115999351981	33.0	33.0	33.0	27.0	33.0
4	31.98277612919286	33.0	33.0	33.0	33.0	33.0
5	32.08216209167888	33.0	33.0	33.0	33.0	33.0
6	35.26068737429814	37.0	37.0	37.0	33.0	37.0
7	35.39291288490946	37.0	37.0	37.0	33.0	37.0
8	35.51737797288594	37.0	37.0	37.0	33.0	37.0
9	35.62788496872518	37.0	37.0	37.0	33.0	37.0
10	35.6354754839398	37.0	37.0	37.0	33.0	37.0
11	35.648020973688055	37.0	37.0	37.0	33.0	37.0
12	35.637846285152065	37.0	37.0	37.0	33.0	37.0
13	35.64427115643732	37.0	37.0	37.0	33.0	37.0
14	35.61985190395094	37.0	37.0	37.0	33.0	37.0
15	35.64365869945748	37.0	37.0	37.0	33.0	37.0
16	35.632128702895145	37.0	37.0	37.0	33.0	37.0
17	35.620375455885316	37.0	37.0	37.0	33.0	37.0
18	35.61704843151743	37.0	37.0	37.0	33.0	37.0
19	35.6054492865864	37.0	37.0	37.0	33.0	37.0
20	35.608614306204785	37.0	37.0	37.0	33.0	37.0
21	35.610912007713004	37.0	37.0	37.0	33.0	37.0
22	35.51438286068777	37.0	37.0	37.0	33.0	37.0
23	35.56731099775169	37.0	37.0	37.0	33.0	37.0
24	35.540349060965156	37.0	37.0	37.0	33.0	37.0
25	35.55444742550745	37.0	37.0	37.0	33.0	37.0
26	35.448642518739206	37.0	37.0	37.0	33.0	37.0
27	35.47071270235776	37.0	37.0	37.0	33.0	37.0
28	35.48413538855456	37.0	37.0	37.0	33.0	37.0
29	35.478006867420845	37.0	37.0	37.0	33.0	37.0
30	35.495505356035075	37.0	37.0	37.0	33.0	37.0
31	35.491461164300475	37.0	37.0	37.0	33.0	37.0
32	35.4697861142173	37.0	37.0	37.0	33.0	37.0
33	35.466664559287814	37.0	37.0	37.0	33.0	37.0
34	35.448433097965456	37.0	37.0	37.0	33.0	37.0
35	35.37873351799241	37.0	37.0	37.0	33.0	37.0
36	35.40473725595565	37.0	37.0	37.0	33.0	37.0
37	35.39212656917405	37.0	37.0	37.0	33.0	37.0
38	35.39253750805084	37.0	37.0	37.0	33.0	37.0
39	35.287295666570515	37.0	37.0	37.0	33.0	37.0
40	35.06773971763758	37.0	37.0	37.0	33.0	37.0
41	35.236536812615824	37.0	37.0	37.0	33.0	37.0
42	35.29052588322224	37.0	37.0	37.0	33.0	37.0
43	34.947670490242174	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	19.0
16	28.0
17	13.0
18	15.0
19	19.0
20	22.0
21	74.0
22	242.0
23	542.0
24	1124.0
25	1999.0
26	3278.0
27	5057.0
28	7160.0
29	9532.0
30	12062.0
31	15951.0
32	19983.0
33	26444.0
34	38146.0
35	78228.0
36	286214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.52173432011348	21.568364028623474	14.046009348859448	23.863892302403595
2	15.284752982270359	22.32800825038822	40.01734636220311	22.369892405138316
3	17.3161344876501	28.11671454368004	31.2011269208429	23.366024047826965
4	12.050387428431439	18.300807257812778	40.91825082286559	28.7305544908902
5	12.868906546967548	38.013426637532156	36.152150119132756	12.965516696367537
6	27.668040414257998	41.426392549362056	17.94360654183081	12.961960494549132
7	24.850343173475476	33.29671762572161	24.56742756214463	17.285511638658285
8	23.728559066536537	36.44889540420185	21.253245034159292	18.56930049510232
9	25.276099557845573	15.713472868155792	21.051134230813304	37.95929334318533
10	14.06813682684063	28.800295559884464	34.98235728764536	22.149210325629547
11	32.369141651421096	24.663840144776927	25.144915224099982	17.822102979701988
12	21.60590171448441	26.616787643384082	31.418055231765575	20.359255410365932
13	27.042148894218805	23.003884162652767	27.47185661394268	22.482110329185748
14	21.329900940022682	22.121155844617686	28.9324677274685	27.616475487891133
15	22.963580542044184	29.660106132867604	25.544790361902802	21.83152296318541
16	21.75763299206967	29.618024411349815	26.043844017085576	22.58049857949494
17	21.246922897593244	28.59877745684154	27.51196266778358	22.64233697778164
18	21.846538037529783	27.917567241849383	29.052983455758874	21.182911264861957
19	23.517162625109155	27.253347768878495	29.06286179414333	20.16662781186902
20	23.315644522066233	26.81889844673007	29.05456398990039	20.81089304130331
21	21.812358986719563	28.01318955741093	28.614187664721292	21.56026379114822
22	21.536358212257834	27.809498219923423	28.66733312522967	21.98681044258907
23	21.549200052157627	27.785987774568415	29.11857562263167	21.546236550642288
24	22.03817780218825	27.92803828053691	28.1846775117651	21.84910640550974
25	22.010320887944083	27.689377625168426	28.94847063565132	21.351830851236176
26	21.827176494296246	27.80851038608498	28.806420129682824	21.557892989935947
27	21.94137008602057	27.70538053335125	28.310329976015396	22.04291940461279
28	21.485781119729413	27.849209140228943	28.912908617467277	21.752101122574373
29	22.45188261372931	27.653025339913622	28.418991698244422	21.476100348112645
30	22.037189968349804	27.842689436895196	28.65330588472374	21.466814710031255
31	21.99649121420584	27.52559477475413	28.72640558876872	21.751508422271307
32	21.13924900920266	27.52717530889564	29.022558173534748	22.311017508366955
33	21.215312214762978	27.567478929504226	29.327798829614473	21.889410026118327
34	21.574488598421837	27.543573350613844	28.848699417968305	22.03323863299602
35	21.733332279643903	28.05803721367636	28.64915698260227	21.559473524077465
36	22.165015667044678	27.744301186586007	28.420967365921314	21.669715780448
37	22.296200000790268	27.296812457770102	28.326530450965905	22.080457090473725
38	22.166398634418503	27.799422314771277	28.298278403186355	21.735900647623865
39	21.55453435488523	27.454075604850658	28.964473543834142	22.02691649642997
40	21.747952220452902	27.86758284962403	28.827362207057877	21.557102722865192
41	21.53932171377317	27.492601124550042	28.76394327462966	22.204133887047128
42	21.48834948770937	27.90018136629274	28.68471900078632	21.926750145211575
43	21.718909905602597	27.3823588681795	28.66081342189593	22.23791780432197
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	47.5
2	61.0
3	182.5
4	304.0
5	304.0
6	385.0
7	466.0
8	425.5
9	385.0
10	581.0
11	777.0
12	777.0
13	1198.5
14	1620.0
15	2954.0
16	4288.0
17	5001.0
18	5714.0
19	5714.0
20	5715.5
21	5717.0
22	6888.0
23	8059.0
24	9837.0
25	11615.0
26	11615.0
27	13711.0
28	15807.0
29	18369.5
30	20932.0
31	22752.0
32	24572.0
33	24572.0
34	27067.0
35	29562.0
36	30991.0
37	32420.0
38	34166.0
39	35912.0
40	35912.0
41	37147.0
42	38382.0
43	37498.5
44	36615.0
45	36415.0
46	36215.0
47	36215.0
48	34950.0
49	33685.0
50	33987.0
51	34289.0
52	33457.5
53	32626.0
54	32626.0
55	29475.5
56	26325.0
57	23247.0
58	20169.0
59	18049.5
60	15930.0
61	15930.0
62	13966.0
63	12002.0
64	9838.0
65	7674.0
66	6379.5
67	5085.0
68	5085.0
69	4223.0
70	3361.0
71	2859.0
72	2357.0
73	1918.0
74	1479.0
75	1479.0
76	1092.5
77	706.0
78	563.5
79	421.0
80	320.5
81	220.0
82	220.0
83	178.0
84	136.0
85	123.0
86	110.0
87	94.0
88	78.0
89	78.0
90	58.0
91	38.0
92	24.0
93	10.0
94	5.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	506158.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.06637222350111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.53361118253903	55.83493677128727
2	6.658707444575948	8.398810444540493
3	1.5978721999284962	3.023160087788254
4	0.7376800956088048	1.8609122996613108
5	0.4196375972238658	1.3232510452747976
6	0.3082147725716055	1.1662792543069567
7	0.21293074245789123	0.9400138623173074
8	0.17325966037090493	0.8741486585815099
9	0.13674645228475532	0.7761692394030252
>10	1.042151894761582	13.592533688808375
>50	0.12378532089373931	5.30275982582229
>100	0.054136290799716796	5.646174335064818
>500	3.165864959047765E-4	0.10242538399123911
>1k	9.497594877143295E-4	1.158425103152377
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2756	0.5444940117512713	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2018	0.39868973719668566	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1028	0.2030986371844365	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	513	0.10135175182452912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.951335353782811E-4	0.0	0.0
2	0.0	0.0	3.951335353782811E-4	0.0	0.0
3	0.0	0.0	3.951335353782811E-4	0.0	0.0
4	0.0	0.0	3.951335353782811E-4	0.0	0.0
5	0.0	0.0	7.902670707565622E-4	0.0	0.0
6	0.0	0.0	0.0019756676768914053	0.0	0.0
7	0.0	0.0	0.0037537685860936703	1.9756676768914054E-4	0.0
8	1.9756676768914054E-4	0.0	0.004346468889161092	1.9756676768914054E-4	0.0
9	1.9756676768914054E-4	0.0	0.004939169192228514	5.927003030674217E-4	0.0
10	3.951335353782811E-4	0.0	0.005729436262985076	7.902670707565622E-4	0.0
11	3.951335353782811E-4	0.0	0.009680771616767886	7.902670707565622E-4	0.0
12	3.951335353782811E-4	0.0	0.01106373899059187	7.902670707565622E-4	0.0
13	3.951335353782811E-4	0.0	0.011656439293659292	7.902670707565622E-4	0.0
14	3.951335353782811E-4	0.0	0.012841839899794136	7.902670707565622E-4	0.0
15	3.951335353782811E-4	0.0	0.01481750757668554	9.878338384457027E-4	0.0
16	3.951335353782811E-4	0.0	0.016002908182820384	9.878338384457027E-4	1.9756676768914054E-4
17	3.951335353782811E-4	0.0	0.016200474950509525	0.0011854006061348434	1.9756676768914054E-4
18	3.951335353782811E-4	0.0	0.016398041718198666	0.0013829673738239838	1.9756676768914054E-4
19	3.951335353782811E-4	0.0	0.016793175253576945	0.0013829673738239838	1.9756676768914054E-4
20	5.927003030674217E-4	0.0	0.017188308788955227	0.0015805341415131243	1.9756676768914054E-4
21	5.927003030674217E-4	0.0	0.017188308788955227	0.0015805341415131243	1.9756676768914054E-4
22	5.927003030674217E-4	0.0	0.017385875556644368	0.002568367979958827	1.9756676768914054E-4
23	5.927003030674217E-4	0.0	0.01758344232433351	0.0031610682830262487	1.9756676768914054E-4
24	5.927003030674217E-4	0.0	0.01758344232433351	0.0035562018184045296	1.9756676768914054E-4
25	5.927003030674217E-4	0.0	0.01758344232433351	0.004346468889161092	1.9756676768914054E-4
26	5.927003030674217E-4	0.0	0.01758344232433351	0.004741602424539373	1.9756676768914054E-4
27	5.927003030674217E-4	0.0	0.01758344232433351	0.006717270101430779	1.9756676768914054E-4
28	5.927003030674217E-4	0.0	0.01758344232433351	0.01837370939509007	1.9756676768914054E-4
29	5.927003030674217E-4	0.0	0.01758344232433351	0.03931578677013897	1.9756676768914054E-4
30	5.927003030674217E-4	0.0	0.01758344232433351	0.06381406596359239	1.9756676768914054E-4
31	5.927003030674217E-4	0.0	0.01758344232433351	0.12545489748260424	1.9756676768914054E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAAC	20	0.0018409628	37.0	22
GTATCAA	1230	0.0	25.268293	1
GCCGGCA	45	1.321783E-4	24.666668	15
GGTATCA	395	0.0	24.35443	1
TCGCCAT	90	3.8162398E-9	22.611113	13
TTAGCCG	85	5.1662937E-8	21.764706	29
TAGCCGG	85	5.1662937E-8	21.764706	30
CGTCCGC	60	3.7209866E-5	21.583332	29
GTCGCCA	95	7.1213435E-9	21.421053	12
CGAGTCG	70	5.0926174E-6	21.142859	21
AGCCGGT	80	6.944283E-7	20.8125	31
CCGGCAG	55	5.13763E-4	20.181818	16
AGCTTCG	55	5.13763E-4	20.181818	21
GCTTCGC	55	5.13763E-4	20.181818	22
TGCTCGC	55	5.13763E-4	20.181818	10
GGACCGT	55	5.13763E-4	20.181818	6
CTAATGC	55	5.13763E-4	20.181818	3
CCGTCCG	65	6.892381E-5	19.923077	28
TGTCCGG	85	1.2424935E-6	19.588236	14
AGAGGTC	105	2.2519089E-8	19.38095	8
>>END_MODULE