FastQCFastQC Report
Fri 10 Feb 2017
ERR1633376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876145
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55350.631744745447386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40250.45939884379868634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22020.2513282618744614No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.12577826729593847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24500.022.0489791
GGTATCA10450.021.775121
TCTAGTA603.7249833E-521.5833342
GTGTTAT953.6044057E-617.5263161
TCGCCCA650.001579528617.07692330
GCGACAA1201.0403346E-716.95833416
GGCGACA1352.2169843E-816.44444515
ACGGTAC1251.6562808E-716.2799993
GCGAACT2050.016.24390423
CATTCGG2750.016.14545437
TTCGCCG1504.663889E-916.03333324
GTCTAAT700.002591834215.8571421
GTACTAG700.002591834215.8571421
GCCGCTC1659.731593E-1015.6969727
CACGGTA1302.586712E-715.6538462
ATTACCC2600.015.6538463
TACCGAG1302.586712E-715.65384626
AGGCGAC1455.3396434E-815.31034514
ATCAACG35550.014.9873433
TCAACGC36050.014.9334264
Produced by FastQC (version 0.11.5)