##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633376.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	876145
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.850024824658018	33.0	33.0	33.0	33.0	33.0
2	31.96952673358862	33.0	33.0	33.0	33.0	33.0
3	31.690495294728613	33.0	33.0	33.0	27.0	33.0
4	31.98010146722289	33.0	33.0	33.0	33.0	33.0
5	32.074681702229654	33.0	33.0	33.0	33.0	33.0
6	35.24502565214662	37.0	37.0	37.0	33.0	37.0
7	35.396113656986	37.0	37.0	37.0	33.0	37.0
8	35.53311495243367	37.0	37.0	37.0	33.0	37.0
9	35.654110906299756	37.0	37.0	37.0	33.0	37.0
10	35.66434094813073	37.0	37.0	37.0	33.0	37.0
11	35.6702703319656	37.0	37.0	37.0	33.0	37.0
12	35.67066638513032	37.0	37.0	37.0	33.0	37.0
13	35.66466851948022	37.0	37.0	37.0	33.0	37.0
14	35.6492669592362	37.0	37.0	37.0	33.0	37.0
15	35.672908023215335	37.0	37.0	37.0	33.0	37.0
16	35.65084204098637	37.0	37.0	37.0	33.0	37.0
17	35.64253976225397	37.0	37.0	37.0	33.0	37.0
18	35.62220865267735	37.0	37.0	37.0	33.0	37.0
19	35.63062620913205	37.0	37.0	37.0	33.0	37.0
20	35.63903691740522	37.0	37.0	37.0	33.0	37.0
21	35.62848386967911	37.0	37.0	37.0	33.0	37.0
22	35.535454747787185	37.0	37.0	37.0	33.0	37.0
23	35.585946390152316	37.0	37.0	37.0	33.0	37.0
24	35.56902339224672	37.0	37.0	37.0	33.0	37.0
25	35.594627601595626	37.0	37.0	37.0	33.0	37.0
26	35.48157439693202	37.0	37.0	37.0	33.0	37.0
27	35.497414240793475	37.0	37.0	37.0	33.0	37.0
28	35.51614287589383	37.0	37.0	37.0	33.0	37.0
29	35.51870067169247	37.0	37.0	37.0	33.0	37.0
30	35.52067066524377	37.0	37.0	37.0	33.0	37.0
31	35.51602645680795	37.0	37.0	37.0	33.0	37.0
32	35.504238453680614	37.0	37.0	37.0	33.0	37.0
33	35.49432342819967	37.0	37.0	37.0	33.0	37.0
34	35.47174953917445	37.0	37.0	37.0	33.0	37.0
35	35.4102722722837	37.0	37.0	37.0	33.0	37.0
36	35.43268751177031	37.0	37.0	37.0	33.0	37.0
37	35.42812890560352	37.0	37.0	37.0	33.0	37.0
38	35.40990817729942	37.0	37.0	37.0	33.0	37.0
39	35.30709871082983	37.0	37.0	37.0	33.0	37.0
40	35.09463730318612	37.0	37.0	37.0	33.0	37.0
41	35.25725764570933	37.0	37.0	37.0	33.0	37.0
42	35.28766243030549	37.0	37.0	37.0	33.0	37.0
43	34.96488594924356	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	22.0
16	35.0
17	37.0
18	27.0
19	26.0
20	44.0
21	148.0
22	358.0
23	883.0
24	1831.0
25	3497.0
26	5567.0
27	8303.0
28	11806.0
29	16259.0
30	21097.0
31	27290.0
32	34616.0
33	45584.0
34	66027.0
35	134302.0
36	498382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34715144182755	20.501172751085722	13.858322537936072	24.293353269150657
2	15.563976282464662	22.027061730649606	39.19796380736065	23.210998179525077
3	17.67960782747148	27.496019494490064	30.474293638610046	24.350079039428405
4	12.164653111071798	17.912902544670118	40.13137094887262	29.791073395385464
5	13.803308813038937	36.981093312180064	35.310137020698626	13.90546085408237
6	28.637383081567545	40.54968070353652	17.55793846908902	13.254997745806914
7	26.007681376941033	32.8128334921731	23.508095121241347	17.671390009644522
8	24.378498992746632	35.639420415570484	21.059527817884028	18.922552773798856
9	25.991816423080653	15.303060566458749	20.65377306267798	38.05134994778261
10	14.710921137482952	28.13689514863407	34.15678911595683	22.99539459792614
11	33.26070456374231	23.882119968726638	24.682558252344077	18.174617215186984
12	22.636892295225106	25.811366839963707	31.001946024915966	20.54979483989522
13	27.77154466441057	22.60676029652626	26.587265806458976	23.034429232604193
14	21.71375742599684	21.24431458263187	28.653019762710507	28.38890822866078
15	23.60328484440361	28.9195281602931	25.23509236484828	22.242094630455004
16	22.167563588218844	29.055578699872736	25.71903052576914	23.05782718613928
17	21.93381232558538	27.88065902333518	26.754133162889705	23.43139548818974
18	22.670448384685184	27.210678597720694	28.55086772166708	21.568005295927044
19	24.06302609727842	26.632463804507246	28.289381323867623	21.01512877434671
20	23.758624428604854	26.255927957130382	28.38582654697567	21.59962106728909
21	22.504722391841533	27.130783146625276	28.43376381763293	21.93073064390027
22	22.398575578243328	26.993248834382438	28.22010055413202	22.388075033242213
23	22.210707131810373	27.052713877269174	28.677330807115258	22.05924818380519
24	22.47390557499044	27.266491277128786	27.776452527835005	22.48315062004577
25	22.57685657054483	26.991194379925698	28.5467588127536	21.885190236775877
26	22.717472564472775	27.26740436799845	28.08587619629171	21.92924687123707
27	22.750343835780605	27.16342614521569	27.945146066005055	22.141083952998645
28	22.15261172522813	27.12188051064607	28.248862916526374	22.476644847599427
29	23.110215774786134	26.916320928613413	27.80156252675071	22.17190076984974
30	22.88856296617569	26.772166707565532	28.414817182087436	21.92445314417134
31	22.66565465761946	26.679830393370963	28.223524644893256	22.430990304116328
32	21.86715669210005	26.830490386865186	28.449628771493302	22.852724149541455
33	22.003777913473225	26.95318697247602	28.75117703119917	22.291858082851583
34	22.346072853237764	26.735871345496463	28.488891678888766	22.429164122377003
35	22.298021446221803	27.283155185500117	28.073549469551274	22.34527389872681
36	22.793144970296012	26.94154506388783	28.1254815127633	22.13982845305286
37	22.558823025869007	26.741806436149268	28.17136432896381	22.528006209017914
38	22.513396755103322	27.084329648631222	27.796768799684983	22.605504796580476
39	22.07865136478551	26.900455974753037	28.299311187075197	22.721581473386255
40	22.31114712747319	27.390215089967985	28.309012777565357	21.989625004993467
41	22.455872030314616	26.673438757283325	28.233682780818242	22.637006431583814
42	22.12578968093181	27.229282824190058	28.210170690924446	22.434756803953686
43	22.568752889076578	26.63725753157297	27.948341884048872	22.845647695301576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	64.5
2	96.0
3	355.0
4	614.0
5	614.0
6	740.5
7	867.0
8	812.5
9	758.0
10	1014.5
11	1271.0
12	1271.0
13	2174.0
14	3077.0
15	5279.0
16	7481.0
17	8560.5
18	9640.0
19	9640.0
20	9053.5
21	8467.0
22	9023.0
23	9579.0
24	12001.0
25	14423.0
26	14423.0
27	17929.0
28	21435.0
29	24944.0
30	28453.0
31	32278.0
32	36103.0
33	36103.0
34	41279.0
35	46455.0
36	49918.5
37	53382.0
38	56764.0
39	60146.0
40	60146.0
41	62038.5
42	63931.0
43	66085.0
44	68239.0
45	67266.5
46	66294.0
47	66294.0
48	65214.0
49	64134.0
50	63879.0
51	63624.0
52	60479.5
53	57335.0
54	57335.0
55	54036.0
56	50737.0
57	44694.0
58	38651.0
59	35747.5
60	32844.0
61	32844.0
62	28423.5
63	24003.0
64	19656.5
65	15310.0
66	13092.0
67	10874.0
68	10874.0
69	8995.0
70	7116.0
71	5906.0
72	4696.0
73	3771.5
74	2847.0
75	2847.0
76	2135.0
77	1423.0
78	1118.5
79	814.0
80	633.5
81	453.0
82	453.0
83	356.0
84	259.0
85	206.5
86	154.0
87	119.5
88	85.0
89	85.0
90	59.5
91	34.0
92	19.0
93	4.0
94	3.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	876145.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.734738302564864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.03561127735955	50.19845247749141
2	9.339477215672892	11.157824547219745
3	2.66608581843763	4.777738159696538
4	1.1266208321334854	2.691936022948464
5	0.6615608315283885	1.975908157128774
6	0.4064935077090844	1.456906998281627
7	0.2863064899766136	1.1971710277155247
8	0.19801122688219505	0.9462519055022174
9	0.15292070517629708	0.8221210474274803
>10	0.9618485521994531	10.896729620429637
>50	0.0973369602824023	4.0713133829464825
>100	0.06385649221990834	7.1421546444528445
>500	0.003096072337715887	1.1785497190506897
>1k	5.805135633217287E-4	0.8471548539547377
>5k	1.9350452110724294E-4	0.6397874357537826
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5535	0.631744745447386	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4025	0.45939884379868634	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2202	0.2513282618744614	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1102	0.12577826729593847	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.282727174154963E-4	0.0	0.0
2	0.0	0.0	2.282727174154963E-4	0.0	0.0
3	0.0	1.1413635870774815E-4	4.565454348309926E-4	0.0	0.0
4	0.0	1.1413635870774815E-4	9.130908696619852E-4	0.0	0.0
5	0.0	1.1413635870774815E-4	0.0013696363044929777	0.0	0.0
6	0.0	1.1413635870774815E-4	0.002396863532862711	1.1413635870774815E-4	0.0
7	0.0	1.1413635870774815E-4	0.003880636196063437	2.282727174154963E-4	0.0
8	0.0	1.1413635870774815E-4	0.00490786342443317	2.282727174154963E-4	0.0
9	1.1413635870774815E-4	1.1413635870774815E-4	0.005935090652802903	6.848181522464889E-4	0.0
10	4.565454348309926E-4	1.1413635870774815E-4	0.007076454239880385	6.848181522464889E-4	0.0
11	4.565454348309926E-4	1.1413635870774815E-4	0.012212590381729052	9.130908696619852E-4	0.0
12	4.565454348309926E-4	1.1413635870774815E-4	0.013696363044929778	0.0010272272283697334	0.0
13	4.565454348309926E-4	1.1413635870774815E-4	0.014495317555884015	0.0010272272283697334	0.0
14	4.565454348309926E-4	1.1413635870774815E-4	0.01597909021908474	0.0010272272283697334	0.0
15	4.565454348309926E-4	1.1413635870774815E-4	0.016892181088746724	0.0012554999457852296	0.0
16	4.565454348309926E-4	1.1413635870774815E-4	0.017691135599700963	0.001597909021908474	0.0
17	4.565454348309926E-4	1.1413635870774815E-4	0.018033544675824208	0.0018261817393239704	0.0
18	5.706817935387408E-4	1.1413635870774815E-4	0.018375953751947453	0.002054454456739467	0.0
19	5.706817935387408E-4	1.1413635870774815E-4	0.0184900901106552	0.0021685908154472147	0.0
20	5.706817935387408E-4	1.1413635870774815E-4	0.018718362828070698	0.0026251362502782076	0.0
21	5.706817935387408E-4	1.1413635870774815E-4	0.019289044621609436	0.002853408967693704	0.0
22	5.706817935387408E-4	1.1413635870774815E-4	0.019859726415148177	0.0030816816851092	0.0
23	5.706817935387408E-4	1.1413635870774815E-4	0.019973862773855926	0.0036523634786479408	0.0
24	5.706817935387408E-4	1.1413635870774815E-4	0.020087999132563674	0.004337181630894429	0.0
25	5.706817935387408E-4	1.1413635870774815E-4	0.020202135491271422	0.004793727065725422	0.0
26	5.706817935387408E-4	1.1413635870774815E-4	0.02031627184997917	0.005250272500556415	0.0
27	5.706817935387408E-4	1.1413635870774815E-4	0.02031627184997917	0.007647136033419126	0.0
28	5.706817935387408E-4	1.1413635870774815E-4	0.02043040820868692	0.018261817393239704	0.0
29	6.848181522464889E-4	1.1413635870774815E-4	0.02043040820868692	0.040404270982542845	0.0
30	6.848181522464889E-4	1.1413635870774815E-4	0.02043040820868692	0.07293313321425107	0.0
31	6.848181522464889E-4	1.1413635870774815E-4	0.02043040820868692	0.14095840300406895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2450	0.0	22.048979	1
GGTATCA	1045	0.0	21.77512	1
TCTAGTA	60	3.7249833E-5	21.583334	2
GTGTTAT	95	3.6044057E-6	17.526316	1
TCGCCCA	65	0.0015795286	17.076923	30
GCGACAA	120	1.0403346E-7	16.958334	16
GGCGACA	135	2.2169843E-8	16.444445	15
ACGGTAC	125	1.6562808E-7	16.279999	3
GCGAACT	205	0.0	16.243904	23
CATTCGG	275	0.0	16.145454	37
TTCGCCG	150	4.663889E-9	16.033333	24
GTCTAAT	70	0.0025918342	15.857142	1
GTACTAG	70	0.0025918342	15.857142	1
GCCGCTC	165	9.731593E-10	15.69697	27
CACGGTA	130	2.586712E-7	15.653846	2
ATTACCC	260	0.0	15.653846	3
TACCGAG	130	2.586712E-7	15.653846	26
AGGCGAC	145	5.3396434E-8	15.310345	14
ATCAACG	3555	0.0	14.987343	3
TCAACGC	3605	0.0	14.933426	4
>>END_MODULE