FastQCFastQC Report
Fri 10 Feb 2017
ERR1633375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862634
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63870.7404067078274216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45040.5221217805001889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25440.2949107037283483No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12270.14223877101992272No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT9150.10607047716644602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT200.001841681737.010
CGAAACT250.005495594329.59999814
TAGACCG250.005495594329.5999985
GGTATCA10000.026.0851
GTATCAA27100.022.800741
GAAGACG450.003825045220.5555576
AGACCGT656.899578E-519.9230776
TCGCCAT1153.0468073E-919.30434813
GTCGCCA1103.848254E-818.512
CACACGT500.00703349618.4999987
CGCTCTC1258.569259E-917.7629
GTCGCCC1705.456968E-1217.41176437
CCGCTCT1301.3915269E-817.07692328
TATTAGA2400.016.9583342
CCGTACA2650.016.75471717
GGTAACG1005.8743735E-616.6525
TGCGGTA803.381342E-416.187536
AACGGCC1951.8189894E-1216.12820637
GCCGGCA1751.3096724E-1015.85714215
GCCGCTC1403.4691766E-815.85714227
Produced by FastQC (version 0.11.5)