FastQCFastQC Report
Fri 10 Feb 2017
ERR1633374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553408
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT158052.855939921360009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT123952.2397580085578817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67321.2164623568867816No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA31420.5677547126170925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28870.5216765930380479No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA24770.44759020469527006No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG24030.4342185150919394No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG22090.39916300451023473No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT19300.3487481207355152No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA19180.3465797386376778No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17160.31007863999074825No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG15210.2748424309008905No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC15070.27231265178674685No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14520.2623742338383254No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT14500.2620128368220192No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC14410.26038655024864116No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13410.2423166994333295No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12380.22370475309355844No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA12280.22189776801202732No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC12250.22135567248756793No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11930.2155733202266682No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC11920.2153926217185151No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA11920.2153926217185151No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11850.21412773216144326No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC11340.2049121082456343No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11310.20437001272117497No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT10860.19623857985428475No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10850.1960578813461316No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA10800.195154388805366No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10490.1895527350526194No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT10280.18575806638140396No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10040.18142130218572916No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC9880.1785301260552793No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA9630.17401266335145138No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA9470.1711214872210015No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC9440.17057939169654215No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC9400.1698565976639297No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9330.16859170810685786No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC9140.16515843645194864No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8920.16118306927258008No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8880.16046027523996761No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8840.15973748120735515No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8730.15774979761767086No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8650.15630420955244595No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8640.1561235110442928No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.15269023938938361No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8240.14889557071816814No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8140.147088585636637No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8100.14636579160402452No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8050.14546229906325892No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7890.14257112293280907No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7840.14166763039204347No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7620.13769226321267491No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG7510.13570457962299062No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG7450.13462038857407194No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7450.13462038857407194No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7450.13462038857407194No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG7400.13371689603330633No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7340.13263270498438764No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC7300.13190991095177518No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7270.13136781542731582No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7230.13064502139470338No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA7130.1288380363131722No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA7050.12739244824794727No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7020.1268503527234879No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.12432057360934427No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6790.12269428703596623No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT6780.12251358852781312No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC6700.12106800046258817No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT6700.12106800046258817No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6620.11962241239736324No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.11763472880767896No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA6400.11564704521799468No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA6360.11492425118538221No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6340.11456285416907598No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6320.11420145715276976No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6310.11402075864461662No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC6290.11365936162831039No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA6250.11293656759569792No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT6250.11293656759569792No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA6180.1116716780386261No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC6160.11131028102231988No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC6160.11131028102231988No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6160.11131028102231988No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG6120.1105874869897074No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA6000.10841910489187002No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5990.10823840638371689No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5950.10751561235110443No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.10733491384295132No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT5810.1049858332369608No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG5800.10480513472880768No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5740.10372094367988897No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG5710.10317884815542963No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5700.10299814964727652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC352.3860866E-531.7142873
CTTCCCT250.00549391229.635
TCCCTGT250.00549391229.637
GAATACA250.00549391229.621
CTGGTGG250.00549391229.627
TACATAG250.00549391229.624
ACCACAT250.00549391229.68
ATACATA250.00549391229.623
TGGTAGA250.00549391229.636
AACAACG405.9346505E-527.752
TAACAAC405.9346505E-527.751
TCCTGCT358.86356E-426.42857237
AAAATAA358.86356E-426.42857236
GAGGTCG951.0913936E-1125.3157926
CGAGGTC951.0913936E-1125.3157925
GGTATCA27050.024.1423281
TCTTTGT1002.0008883E-1124.05000137
AGGTCGC1002.0008883E-1124.05000127
GGTCGCT952.8012437E-1023.36842228
GCTCTCG1054.0017767E-1122.9047618
Produced by FastQC (version 0.11.5)