FastQCFastQC Report
Fri 10 Feb 2017
ERR1633373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980424
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72500.7394759818201104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51340.5236509918157858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28930.295076415918011No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC13670.1394294713307712No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.1325956932918819No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12400.12647589206302579No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11340.11566424322538005No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11290.11515425978964203No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11240.11464427635390403No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10640.10852447512504793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCG250.00549595429.69
CGTTATT405.9392514E-527.75000210
CGCCGTT706.5901986E-926.4285725
GGTATCA13950.024.1362021
CCGTTTA851.9754225E-923.94117727
GCTATAC551.9022887E-523.5454543
GGACCGT903.8271537E-922.6111136
AGGACCG1201.0913936E-1121.5833325
GTATCAA33650.020.9465071
TTTACCG909.474752E-820.55555730
CGACCTT450.003825412120.55555725
TTACCGG909.474752E-820.55555731
GCCGGCA3050.020.01639415
CGCTCTC2700.019.87036929
TAGGACC2800.019.8214284
GACCGTT1052.2584572E-819.380957
TCGCTGC1153.0486262E-919.30434825
CCGGCAG3000.019.11666716
GCCGCTC3000.019.11666727
TTCGCCG3000.019.11666724
Produced by FastQC (version 0.11.5)