##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633373.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	980424
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78666576909582	33.0	33.0	33.0	27.0	33.0
2	31.91041528971139	33.0	33.0	33.0	33.0	33.0
3	31.628643321664914	33.0	33.0	33.0	27.0	33.0
4	31.92782408427374	33.0	33.0	33.0	33.0	33.0
5	32.0328296736922	33.0	33.0	33.0	33.0	33.0
6	35.161109887150864	37.0	37.0	37.0	33.0	37.0
7	35.312855458454706	37.0	37.0	37.0	33.0	37.0
8	35.45182390475957	37.0	37.0	37.0	33.0	37.0
9	35.566795590479224	37.0	37.0	37.0	33.0	37.0
10	35.561929328535406	37.0	37.0	37.0	33.0	37.0
11	35.582973285027705	37.0	37.0	37.0	33.0	37.0
12	35.57393127871207	37.0	37.0	37.0	33.0	37.0
13	35.57287765293383	37.0	37.0	37.0	33.0	37.0
14	35.558829649212996	37.0	37.0	37.0	33.0	37.0
15	35.58314871932959	37.0	37.0	37.0	33.0	37.0
16	35.56145198403956	37.0	37.0	37.0	33.0	37.0
17	35.54723262588431	37.0	37.0	37.0	33.0	37.0
18	35.54671550268047	37.0	37.0	37.0	33.0	37.0
19	35.536137426256396	37.0	37.0	37.0	33.0	37.0
20	35.53094783481433	37.0	37.0	37.0	33.0	37.0
21	35.52696792408182	37.0	37.0	37.0	33.0	37.0
22	35.42792302106027	37.0	37.0	37.0	33.0	37.0
23	35.480712426460386	37.0	37.0	37.0	33.0	37.0
24	35.469213319951365	37.0	37.0	37.0	33.0	37.0
25	35.4876063825447	37.0	37.0	37.0	33.0	37.0
26	35.37768047293824	37.0	37.0	37.0	33.0	37.0
27	35.38147474970013	37.0	37.0	37.0	33.0	37.0
28	35.39691296826679	37.0	37.0	37.0	33.0	37.0
29	35.4001381035144	37.0	37.0	37.0	33.0	37.0
30	35.40466471649001	37.0	37.0	37.0	33.0	37.0
31	35.38814023320523	37.0	37.0	37.0	33.0	37.0
32	35.38296900116684	37.0	37.0	37.0	33.0	37.0
33	35.3682060006691	37.0	37.0	37.0	33.0	37.0
34	35.34863283640547	37.0	37.0	37.0	33.0	37.0
35	35.280749961241256	37.0	37.0	37.0	33.0	37.0
36	35.30980167764151	37.0	37.0	37.0	33.0	37.0
37	35.29466026943445	37.0	37.0	37.0	33.0	37.0
38	35.27659155630625	37.0	37.0	37.0	33.0	37.0
39	35.1726691717053	37.0	37.0	37.0	33.0	37.0
40	34.95942265795207	37.0	37.0	37.0	27.0	37.0
41	35.113703866898405	37.0	37.0	37.0	27.0	37.0
42	35.14928949107733	37.0	37.0	37.0	33.0	37.0
43	34.794574592217245	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	29.0
16	47.0
17	35.0
18	43.0
19	39.0
20	76.0
21	226.0
22	508.0
23	1258.0
24	2481.0
25	4360.0
26	7093.0
27	10705.0
28	14814.0
29	19641.0
30	25439.0
31	32225.0
32	40865.0
33	53071.0
34	75720.0
35	152072.0
36	539674.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10627238827283	20.58221748957594	13.716208497548	23.59530162460323
2	16.108744787969286	22.063107390271963	39.169481775231944	22.65866604652681
3	17.668886114579	28.020631889876217	30.062095583135463	24.248386412409324
4	11.966251336156601	18.24577937708583	40.46351374507356	29.324455541684006
5	13.767410834496097	37.036119066852706	35.226391846792815	13.970078251858379
6	28.403833443489756	40.89353177808785	17.245905852977895	13.456728925444503
7	26.392050786190463	32.54265501456513	23.39651008135256	17.66878411789185
8	24.44972787283869	35.23495956851321	20.734600540174455	19.58071201847364
9	26.29097206922719	15.017176242115656	20.549068566252966	38.14278312240418
10	15.36274101817173	27.733511215555716	33.72755052915881	23.176197237113737
11	33.54915832333766	24.270927680268944	23.851313309343713	18.328600687049683
12	23.058901046893997	26.258537122714255	30.055567795158012	20.626994035233736
13	27.938116569973808	22.810947100438177	26.34676425709693	22.904172072491086
14	22.30432955537604	21.399210953628227	28.347837262245722	27.948622228750008
15	23.998698522271997	28.69187208799458	25.58178910348992	21.727640286243503
16	22.563299144043803	28.674736644553782	25.539766468385107	23.222197743017308
17	22.251291278059288	27.27248618964856	26.366449617716412	24.109772914575732
18	22.71262229402789	26.685495255114112	28.686262270201464	21.915620180656532
19	25.157584881643047	25.96111478299185	28.251450392891236	20.629849942473868
20	24.408317217856766	25.885637234502624	28.368236599675246	21.33780894796537
21	22.61093159694173	27.054825259275578	28.272359713756494	22.061883430026192
22	22.674985516470425	26.523932502672317	28.321114130213047	22.47996785064421
23	22.17764966993872	26.617361468099514	29.118626227020144	22.08636263494162
24	22.54585771054156	27.36091731740553	27.421401352884057	22.671823619168848
25	22.598895987858313	27.034527918533207	28.743380415004122	21.62319567860436
26	23.122648976361248	26.88653072548204	28.09784338204695	21.892976916109767
27	22.872655096162475	26.871027229035597	27.640184246815664	22.61613342798626
28	22.1934591564466	26.648878444428124	28.706253620882393	22.451408778242882
29	23.74666470833027	26.69079908284579	27.79807511852015	21.76446109030379
30	23.26758626879799	26.326160926293113	28.287761213515783	22.11849159139311
31	22.61103359362888	26.379607190358456	28.378844255138592	22.63051496087407
32	21.577909149510823	26.442131159579933	28.7208391471445	23.259120543764737
33	21.803117834732728	26.33350468776774	29.117912250210114	22.74546522728942
34	22.6612159637055	26.363185723727696	28.76724763979666	22.20835067277015
35	22.5442257635472	27.13774856592658	27.853153329579854	22.464872340946364
36	23.142334336980735	26.7561789593074	27.699342325361272	22.40214437835059
37	22.81604693479556	26.255171232038382	28.205347890300526	22.723433942865537
38	22.727717803725735	26.46181652019942	27.675067113820145	23.135398562254696
39	22.2551671521709	26.52791037347107	28.32509200101181	22.891830473346225
40	22.517400634827382	27.399370068460176	28.229929091903095	21.853300204809347
41	22.607361712891567	26.25476324528979	28.180256705262213	22.95761833655643
42	21.822395208603623	27.672007213205713	28.039705270372817	22.465892307817843
43	23.095211867518543	26.055767708664824	27.74320090083474	23.105819522981893
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	81.0
2	111.0
3	398.5
4	686.0
5	686.0
6	877.0
7	1068.0
8	1013.5
9	959.0
10	1293.0
11	1627.0
12	1627.0
13	2568.5
14	3510.0
15	6334.5
16	9159.0
17	10341.5
18	11524.0
19	11524.0
20	10340.0
21	9156.0
22	9230.5
23	9305.0
24	11538.5
25	13772.0
26	13772.0
27	16968.5
28	20165.0
29	23599.0
30	27033.0
31	31519.5
32	36006.0
33	36006.0
34	44698.5
35	53391.0
36	57579.5
37	61768.0
38	64647.0
39	67526.0
40	67526.0
41	69756.5
42	71987.0
43	74422.5
44	76858.0
45	75535.5
46	74213.0
47	74213.0
48	72511.0
49	70809.0
50	72022.0
51	73235.0
52	71123.5
53	69012.0
54	69012.0
55	65992.0
56	62972.0
57	53215.0
58	43458.0
59	39518.5
60	35579.0
61	35579.0
62	31542.5
63	27506.0
64	21599.0
65	15692.0
66	13298.0
67	10904.0
68	10904.0
69	9185.5
70	7467.0
71	6787.0
72	6107.0
73	5302.0
74	4497.0
75	4497.0
76	2911.0
77	1325.0
78	1066.0
79	807.0
80	622.5
81	438.0
82	438.0
83	378.5
84	319.0
85	270.0
86	221.0
87	170.5
88	120.0
89	120.0
90	96.5
91	73.0
92	39.5
93	6.0
94	4.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	980424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.93831614256372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14653897336694	42.520747816971216
2	8.432134569822413	8.421732038088644
3	2.1817029680955407	3.2685171764977414
4	0.9809420938790386	1.9594638560671944
5	0.5713935633319044	1.4267216203747324
6	0.399463720342605	1.1969127332372202
7	0.29135752233398593	1.0184932842580063
8	0.22862454202142193	0.9133699725931692
9	0.17164811803509913	0.7714636185341588
>10	1.273092671370613	12.770158711445257
>50	0.16913614374987562	5.9373420149515
>100	0.14170608301814552	13.797119182020149
>500	0.010181228830388049	3.545544918336057
>1k	0.001662241441696008	1.167425398776675
>5k	4.15560360424002E-4	1.2849876578482218
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7250	0.7394759818201104	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5134	0.5236509918157858	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2893	0.295076415918011	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1367	0.1394294713307712	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1300	0.1325956932918819	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1240	0.12647589206302579	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1134	0.11566424322538005	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1129	0.11515425978964203	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1124	0.11464427635390403	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1064	0.10852447512504793	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.039933742952029E-4	0.0	0.0
2	0.0	0.0	2.039933742952029E-4	1.0199668714760145E-4	0.0
3	0.0	0.0	4.079867485904058E-4	1.0199668714760145E-4	0.0
4	0.0	0.0	4.079867485904058E-4	1.0199668714760145E-4	0.0
5	0.0	0.0	6.119801228856086E-4	1.0199668714760145E-4	0.0
6	0.0	0.0	0.0010199668714760144	1.0199668714760145E-4	0.0
7	0.0	0.0	0.0032638939887232464	2.039933742952029E-4	0.0
8	0.0	0.0	0.003671880737313652	2.039933742952029E-4	0.0
9	0.0	0.0	0.004997837670232471	7.139768100332102E-4	0.0
10	4.079867485904058E-4	0.0	0.005915807854560884	7.139768100332102E-4	0.0
11	4.079867485904058E-4	0.0	0.00856772172039852	7.139768100332102E-4	0.0
12	4.079867485904058E-4	0.0	0.009995675340464942	7.139768100332102E-4	0.0
13	4.079867485904058E-4	0.0	0.011015642211940957	7.139768100332102E-4	0.0
14	4.079867485904058E-4	0.0	0.011729619021974166	7.139768100332102E-4	0.0
15	4.079867485904058E-4	0.0	0.013259569329188188	7.139768100332102E-4	0.0
16	4.079867485904058E-4	0.0	0.014585526262107006	7.139768100332102E-4	0.0
17	5.099834357380072E-4	0.0	0.014993513010697412	7.139768100332102E-4	0.0
18	6.119801228856086E-4	0.0	0.015707489820730624	7.139768100332102E-4	0.0
19	7.139768100332102E-4	0.0	0.015911483195025826	7.139768100332102E-4	0.0
20	7.139768100332102E-4	0.0	0.016115476569321028	8.159734971808116E-4	0.0
21	7.139768100332102E-4	0.0	0.016625460005059035	0.001325956932918819	0.0
22	8.159734971808116E-4	0.0	0.01682945337935424	0.0019379370558044275	0.0
23	8.159734971808116E-4	0.0	0.01693145006650184	0.0025499171786900364	0.0
24	8.159734971808116E-4	0.0	0.01693145006650184	0.0037738774244612536	0.0
25	9.17970184328413E-4	0.0	0.01693145006650184	0.004589850921642065	0.0
26	9.17970184328413E-4	0.0	0.01693145006650184	0.005711814480265681	0.0
27	0.0010199668714760144	0.0	0.01693145006650184	0.006935774726036899	0.0
28	0.0010199668714760144	0.0	0.01693145006650184	0.01693145006650184	1.0199668714760145E-4
29	0.0010199668714760144	0.0	0.01693145006650184	0.039880704674712164	1.0199668714760145E-4
30	0.0010199668714760144	0.0	0.01693145006650184	0.069867730696107	1.0199668714760145E-4
31	0.0010199668714760144	0.0	0.01693145006650184	0.1404494382022472	1.0199668714760145E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGCGCG	25	0.005495954	29.6	9
CGTTATT	40	5.9392514E-5	27.750002	10
CGCCGTT	70	6.5901986E-9	26.42857	25
GGTATCA	1395	0.0	24.136202	1
CCGTTTA	85	1.9754225E-9	23.941177	27
GCTATAC	55	1.9022887E-5	23.545454	3
GGACCGT	90	3.8271537E-9	22.611113	6
AGGACCG	120	1.0913936E-11	21.583332	5
GTATCAA	3365	0.0	20.946507	1
TTTACCG	90	9.474752E-8	20.555557	30
CGACCTT	45	0.0038254121	20.555557	25
TTACCGG	90	9.474752E-8	20.555557	31
GCCGGCA	305	0.0	20.016394	15
CGCTCTC	270	0.0	19.870369	29
TAGGACC	280	0.0	19.821428	4
GACCGTT	105	2.2584572E-8	19.38095	7
TCGCTGC	115	3.0486262E-9	19.304348	25
CCGGCAG	300	0.0	19.116667	16
GCCGCTC	300	0.0	19.116667	27
TTCGCCG	300	0.0	19.116667	24
>>END_MODULE