FastQCFastQC Report
Fri 10 Feb 2017
ERR1633372.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633372.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998922
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46190.46239846554585845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32170.3220471668458598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18440.18459899771954166No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC17300.17318669525748756No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC16280.16297568779143917No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG14630.1464578815963609No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA12960.12973985956861497No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12940.12953964373594737No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG11880.11892820460456371No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11550.11562464336554805No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10760.10771611797517723No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10190.10200996674415021No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT451.323043E-424.66666625
GTATCAA23450.024.0618341
GGTATCA8150.024.061351
TTTACCG400.001931011223.12530
CCGCCGT603.7256563E-521.58333424
TGCGGTA806.958235E-720.812536
ACGGTTC656.900975E-519.9230778
AGGACCG701.21899444E-418.55
GTTACGG609.235659E-418.517
GGACCGT801.6163252E-518.56
CCGTTTA609.235659E-418.527
CGAACCG2850.018.17543831
GTACATG3500.017.9714281
GCTTAAT1054.795711E-717.6190471
TAGGACC3000.017.2666664
ATACCGC752.0669244E-417.2666666
TAGACTG1301.3924364E-817.0769235
GCGAACC3050.016.98360630
ATAGACT1107.8050834E-716.8181824
GTATTAG5750.016.7304341
Produced by FastQC (version 0.11.5)