##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633372.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	998922
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80167220263444	33.0	33.0	33.0	27.0	33.0
2	31.931037658595965	33.0	33.0	33.0	33.0	33.0
3	31.65502311491788	33.0	33.0	33.0	27.0	33.0
4	31.938243426413674	33.0	33.0	33.0	33.0	33.0
5	32.040651822664834	33.0	33.0	33.0	33.0	33.0
6	35.18805772622888	37.0	37.0	37.0	33.0	37.0
7	35.328422038957996	37.0	37.0	37.0	33.0	37.0
8	35.47065536648507	37.0	37.0	37.0	33.0	37.0
9	35.58835825019371	37.0	37.0	37.0	33.0	37.0
10	35.587575406287975	37.0	37.0	37.0	33.0	37.0
11	35.604216345220145	37.0	37.0	37.0	33.0	37.0
12	35.5892622246782	37.0	37.0	37.0	33.0	37.0
13	35.598237900456695	37.0	37.0	37.0	33.0	37.0
14	35.57718420457253	37.0	37.0	37.0	33.0	37.0
15	35.595780251110696	37.0	37.0	37.0	33.0	37.0
16	35.58104937122218	37.0	37.0	37.0	33.0	37.0
17	35.56139518400836	37.0	37.0	37.0	33.0	37.0
18	35.567658936333366	37.0	37.0	37.0	33.0	37.0
19	35.55360278380094	37.0	37.0	37.0	33.0	37.0
20	35.55547279967805	37.0	37.0	37.0	33.0	37.0
21	35.55017308658734	37.0	37.0	37.0	33.0	37.0
22	35.46402321702796	37.0	37.0	37.0	33.0	37.0
23	35.5166209173489	37.0	37.0	37.0	33.0	37.0
24	35.500160172666135	37.0	37.0	37.0	33.0	37.0
25	35.52299278622355	37.0	37.0	37.0	33.0	37.0
26	35.401737072564224	37.0	37.0	37.0	33.0	37.0
27	35.41428660095583	37.0	37.0	37.0	33.0	37.0
28	35.434045901481795	37.0	37.0	37.0	33.0	37.0
29	35.44561136905584	37.0	37.0	37.0	33.0	37.0
30	35.44863362704996	37.0	37.0	37.0	33.0	37.0
31	35.43842161850475	37.0	37.0	37.0	33.0	37.0
32	35.42790828513137	37.0	37.0	37.0	33.0	37.0
33	35.424553668855026	37.0	37.0	37.0	33.0	37.0
34	35.40570735252602	37.0	37.0	37.0	33.0	37.0
35	35.3441009408142	37.0	37.0	37.0	33.0	37.0
36	35.374041216431316	37.0	37.0	37.0	33.0	37.0
37	35.37286995381021	37.0	37.0	37.0	33.0	37.0
38	35.35987494519092	37.0	37.0	37.0	33.0	37.0
39	35.25627726689371	37.0	37.0	37.0	33.0	37.0
40	35.05305719565692	37.0	37.0	37.0	27.0	37.0
41	35.22885070105574	37.0	37.0	37.0	33.0	37.0
42	35.26961164134937	37.0	37.0	37.0	33.0	37.0
43	34.936990075301175	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	19.0
16	47.0
17	46.0
18	38.0
19	36.0
20	66.0
21	183.0
22	450.0
23	1134.0
24	2392.0
25	4285.0
26	6918.0
27	10193.0
28	14429.0
29	19340.0
30	25075.0
31	32332.0
32	40753.0
33	53363.0
34	76429.0
35	154958.0
36	556431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.282902969401015	20.418911586690452	13.532387914171476	24.765797529737057
2	16.331705578613747	21.259717975978106	38.87901157447728	23.529564870930862
3	17.633609030534917	27.39653346307319	29.837464787040428	25.132392719351458
4	12.375841156766995	17.776062595477924	39.961178150045754	29.88691809770933
5	13.733905149751433	37.2782859922997	35.331387235439806	13.656421622509066
6	29.419714452179452	39.94075613511365	17.267814704251183	13.371714708455714
7	26.83022298037284	32.09429765286979	23.50013314352872	17.575346223228642
8	24.662486160080565	35.54982270888017	20.511211085550222	19.276480045489038
9	26.303154800875344	15.318012817817609	20.191666616612707	38.18716576469434
10	15.479687102696706	27.5727233958207	33.54386028138333	23.40372922009927
11	33.64837294603583	23.952020277859532	23.99286430772373	18.406742468380916
12	23.42565285377637	25.876394753544318	29.638049817703482	21.059902574975826
13	27.534882603446516	22.149377028436653	26.069102492486902	24.24663787562993
14	22.078700839504986	20.904234765076755	27.8264969637269	29.19056743169136
15	24.22070992529947	28.331441293714626	24.80243702711523	22.645411753870672
16	22.813793269144135	28.199899491652005	25.033185774264656	23.953121464939205
17	22.35569944400063	27.3918283910055	26.21916425907128	24.033307905922584
18	22.851934385267317	26.996702445235965	27.90488146221627	22.24648170728045
19	25.03909214132835	25.83965514824981	27.32575716622519	21.795495544196644
20	24.106386684846264	26.145985372231266	27.52156825057412	22.22605969234835
21	23.432860623752404	26.799289634225694	27.409447384280256	22.358402357741646
22	22.96475600697552	26.716200063668634	27.28020806429331	23.038835865062538
23	22.84642844986896	26.62690380229888	27.88415912353517	22.64250862429699
24	23.208919214913674	27.217039969086677	26.3646210615043	23.209419754495347
25	23.099000722779156	26.638416212677267	27.75211678189088	22.5104662826527
26	23.31813695163386	26.912111255933898	27.609062569449865	22.160689222982374
27	23.270986123040636	26.770458554321557	26.719103193242315	23.239452129395488
28	22.409157071322884	26.4913576835829	28.174772404652217	22.924712840441998
29	24.23012006943485	26.302353937544677	26.95485733620843	22.512668656812043
30	23.503737028516742	26.212156704927914	27.590142173262777	22.69396409329257
31	23.065564678723664	26.340194729918853	27.65901641970044	22.935224171657044
32	22.08801087572403	26.27722685054489	27.82519556081456	23.809566712916524
33	22.329471169921174	26.471936747814144	28.2064065062137	22.992185576050982
34	23.232544683168456	26.36692354357998	27.77814484013767	22.622386933113898
35	23.249062489363535	26.94504676040772	27.227050760720058	22.57883998950869
36	24.040415567982283	26.560632361685897	26.698781286226552	22.700170784105264
37	23.13804281014934	26.100936809881052	27.150968744306365	23.610051635663247
38	23.465696020309895	26.55492621045487	27.048057806315207	22.93131996292003
39	22.520577182202413	26.623900564808867	27.614268181099227	23.241254071889497
40	22.90879568174492	27.006212697287673	27.848821029069338	22.236170591898066
41	22.68455394915719	25.994221771069213	27.851523942810353	23.469700336963246
42	22.276113650515256	27.302432021719415	27.453895299132462	22.967559028632866
43	23.373396521450125	25.717022950740898	27.50004504856235	23.409535479246628
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	51.0
1	59.0
2	67.0
3	268.5
4	470.0
5	470.0
6	567.5
7	665.0
8	622.5
9	580.0
10	794.5
11	1009.0
12	1009.0
13	1646.0
14	2283.0
15	4012.5
16	5742.0
17	6582.0
18	7422.0
19	7422.0
20	6762.5
21	6103.0
22	6527.0
23	6951.0
24	9112.5
25	11274.0
26	11274.0
27	14868.5
28	18463.0
29	22183.0
30	25903.0
31	31263.5
32	36624.0
33	36624.0
34	45282.0
35	53940.0
36	58715.0
37	63490.0
38	67840.0
39	72190.0
40	72190.0
41	76013.0
42	79836.0
43	81590.0
44	83344.0
45	80909.5
46	78475.0
47	78475.0
48	74950.5
49	71426.0
50	71960.5
51	72495.0
52	69554.5
53	66614.0
54	66614.0
55	65305.0
56	63996.0
57	54985.0
58	45974.0
59	41698.5
60	37423.0
61	37423.0
62	33794.0
63	30165.0
64	23842.5
65	17520.0
66	14741.5
67	11963.0
68	11963.0
69	9920.5
70	7878.0
71	6841.0
72	5804.0
73	5869.0
74	5934.0
75	5934.0
76	4950.5
77	3967.0
78	2785.0
79	1603.0
80	1079.5
81	556.0
82	556.0
83	422.5
84	289.0
85	266.5
86	244.0
87	176.5
88	109.0
89	109.0
90	86.5
91	64.0
92	39.5
93	15.0
94	8.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	998922.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.899462884682336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.66851867189897	42.249136049694556
2	8.652587186373468	8.635189063258416
3	2.356508800111188	3.527655702257266
4	1.030581211523518	2.0570179565627495
5	0.5962974276817442	1.4877460680418373
6	0.4039338229958085	1.2093648485068311
7	0.28768056811633913	1.0048574081955914
8	0.20775363123545745	0.8293435688793352
9	0.1707899536328152	0.7670094257139553
>10	1.301745136405764	13.104071919660539
>50	0.16760064975717637	5.857850327263151
>100	0.14278235212936868	13.434744798853734
>500	0.0107798641743884	3.6509841522856323
>1k	0.002440723964012468	2.18502871082638
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4619	0.46239846554585845	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3217	0.3220471668458598	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1844	0.18459899771954166	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1730	0.17318669525748756	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1628	0.16297568779143917	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1463	0.1464578815963609	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1296	0.12973985956861497	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1294	0.12953964373594737	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1188	0.11892820460456371	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1155	0.11562464336554805	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1076	0.10771611797517723	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1019	0.10200996674415021	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.0010791633380785E-4	0.0	0.0
2	0.0	0.0	1.0010791633380785E-4	0.0	0.0
3	0.0	0.0	3.003237490014235E-4	0.0	0.0
4	0.0	0.0	3.003237490014235E-4	0.0	0.0
5	1.0010791633380785E-4	0.0	4.004316653352314E-4	0.0	0.0
6	1.0010791633380785E-4	0.0	5.005395816690393E-4	1.0010791633380785E-4	0.0
7	1.0010791633380785E-4	0.0	0.002002158326676157	1.0010791633380785E-4	0.0
8	1.0010791633380785E-4	0.0	0.002702913741012812	1.0010791633380785E-4	0.0
9	1.0010791633380785E-4	0.0	0.0031033454063480433	4.004316653352314E-4	0.0
10	2.002158326676157E-4	0.0	0.0036038849880170826	4.004316653352314E-4	0.0
11	2.002158326676157E-4	0.0	0.006106582896362279	6.00647498002847E-4	0.0
12	3.003237490014235E-4	0.0	0.006406906645363702	6.00647498002847E-4	0.0
13	3.003237490014235E-4	0.0	0.006607122478031318	8.008633306704628E-4	0.0
14	3.003237490014235E-4	0.0	0.006907446227032741	8.008633306704628E-4	0.0
15	3.003237490014235E-4	0.0	0.007508093725035588	8.008633306704628E-4	0.0
16	3.003237490014235E-4	0.0	0.00790852539037082	8.008633306704628E-4	0.0
17	3.003237490014235E-4	0.0	0.008108741223038436	8.008633306704628E-4	0.0
18	3.003237490014235E-4	0.0	0.008108741223038436	8.008633306704628E-4	0.0
19	3.003237490014235E-4	0.0	0.00840906497203986	9.009712470042706E-4	0.0
20	3.003237490014235E-4	0.0	0.008509172888373667	0.0011011870796718863	0.0
21	3.003237490014235E-4	0.0	0.008509172888373667	0.001201294996005694	1.0010791633380785E-4
22	3.003237490014235E-4	0.0	0.008709388721041282	0.0014015108286733098	1.0010791633380785E-4
23	3.003237490014235E-4	0.0	0.00880949663737509	0.0022023741593437726	1.0010791633380785E-4
24	3.003237490014235E-4	0.0	0.00880949663737509	0.002702913741012812	1.0010791633380785E-4
25	3.003237490014235E-4	0.0	0.00880949663737509	0.0034036691553494666	1.0010791633380785E-4
26	3.003237490014235E-4	0.0	0.00880949663737509	0.004004316653352314	1.0010791633380785E-4
27	3.003237490014235E-4	0.0	0.00880949663737509	0.004805179984022776	1.0010791633380785E-4
28	3.003237490014235E-4	0.0	0.00880949663737509	0.015416619115406408	1.0010791633380785E-4
29	3.003237490014235E-4	0.0	0.00880949663737509	0.03133377781248185	1.0010791633380785E-4
30	3.003237490014235E-4	0.0	0.00880949663737509	0.05726172814293809	1.0010791633380785E-4
31	3.003237490014235E-4	0.0	0.00880949663737509	0.12213165792724558	1.0010791633380785E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGTT	45	1.323043E-4	24.666666	25
GTATCAA	2345	0.0	24.061834	1
GGTATCA	815	0.0	24.06135	1
TTTACCG	40	0.0019310112	23.125	30
CCGCCGT	60	3.7256563E-5	21.583334	24
TGCGGTA	80	6.958235E-7	20.8125	36
ACGGTTC	65	6.900975E-5	19.923077	8
AGGACCG	70	1.21899444E-4	18.5	5
GTTACGG	60	9.235659E-4	18.5	17
GGACCGT	80	1.6163252E-5	18.5	6
CCGTTTA	60	9.235659E-4	18.5	27
CGAACCG	285	0.0	18.175438	31
GTACATG	350	0.0	17.971428	1
GCTTAAT	105	4.795711E-7	17.619047	1
TAGGACC	300	0.0	17.266666	4
ATACCGC	75	2.0669244E-4	17.266666	6
TAGACTG	130	1.3924364E-8	17.076923	5
GCGAACC	305	0.0	16.983606	30
ATAGACT	110	7.8050834E-7	16.818182	4
GTATTAG	575	0.0	16.730434	1
>>END_MODULE