FastQCFastQC Report
Fri 10 Feb 2017
ERR1633371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642697
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50350.7834173801962666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34860.5424017849779912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19860.3090103112353099No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG11740.18266772678260518No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA11290.17566598257032473No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG9250.1439247421413201No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9080.14127963877223637No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG8730.13583383771824048No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7540.11731811413465444No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7460.1160733596080268No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC7390.11498419939722762No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7280.11327266192311464No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT7110.1106275585540309No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7100.11047196423820244No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA7070.1100051812907171No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7070.1100051812907171No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC6870.106893294974148No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT6800.10580413476334882No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6490.10098071097266674No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC6480.1008251166568383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTT250.00549456229.625
TTAACGG1200.024.66666835
GGTATCA7500.023.9266661
GTATCAA20900.022.8373221
GGGTATA502.7003055E-422.21
GCGCACG502.7003055E-422.234
CGCTCTC1800.021.58333229
GCCGGCA1750.021.14285915
CCGCTCT1850.021.028
TTCGCCG1850.021.024
CGAGCAT450.00382420.55555537
TGTACTA450.00382420.5555555
GCGGTAA555.139704E-420.18181823
GCTATAT851.2435594E-619.58823619
AGCTTCG2000.019.42500121
GCTTCGC2000.019.42500122
TAATATA1850.019.04
CCGGCAG2150.018.93023316
GCCCCTA500.007031588318.536
ACGCATT609.230775E-418.56
Produced by FastQC (version 0.11.5)