FastQCFastQC Report
Fri 10 Feb 2017
ERR1633370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426395
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12800.3001911373257191No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9640.2260814502984322No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7050.16533964985518124No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6560.15384795787943104No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6340.14868842270664523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6200.1454050821421452No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5550.13016100094982352No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5470.12828480634153777No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5120.12007645493028764No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5110.11984193060425193No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5000.11726216301785902No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA4810.1128062008231804No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT4790.11233715217110894No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT4650.10905381160660889No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4350.10201808182553734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA303.595714E-430.83333426
CGTCAAA509.073585E-625.90000227
GTATCAA6700.024.8507461
GGTATCA2300.024.1304361
ACGCCGG400.001929206123.12514
GATAGCG400.001929206123.12525
AGCTATA502.6982406E-422.22
TCGCCTA603.7192123E-521.58333435
CGGCGTC603.7192123E-521.58333424
CCGGCGT603.7192123E-521.58333423
TTGCGCC450.003821920620.55555513
CGGTGGG450.003821920620.55555518
TTTGACG450.003821920620.55555510
TCGCCAT1001.2840246E-820.3513
ACACGAT555.135803E-420.18181815
CTCTAAC555.135803E-420.1818181
GCGTCAA656.88912E-519.92307726
CCCACCG759.243373E-619.73333419
CTAGCAC1052.2493623E-819.380953
GTCGCCA1103.8346116E-818.512
Produced by FastQC (version 0.11.5)