##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633370.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426395
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.046531971528747	33.0	33.0	33.0	27.0	33.0
2	31.389835715709612	33.0	33.0	33.0	27.0	33.0
3	31.145318308141512	33.0	33.0	33.0	27.0	33.0
4	31.461377361366807	33.0	33.0	33.0	27.0	33.0
5	31.578125916110647	33.0	33.0	33.0	27.0	33.0
6	34.24640767363595	37.0	33.0	37.0	27.0	37.0
7	34.40006097632477	37.0	37.0	37.0	27.0	37.0
8	34.51243565238804	37.0	37.0	37.0	27.0	37.0
9	34.64530306406032	37.0	37.0	37.0	27.0	37.0
10	34.71053366010389	37.0	37.0	37.0	27.0	37.0
11	34.68505259208011	37.0	37.0	37.0	27.0	37.0
12	34.69392933782056	37.0	37.0	37.0	27.0	37.0
13	34.740963191407026	37.0	37.0	37.0	27.0	37.0
14	34.7067531279682	37.0	37.0	37.0	27.0	37.0
15	34.7299968339216	37.0	37.0	37.0	27.0	37.0
16	34.70025680413701	37.0	37.0	37.0	27.0	37.0
17	34.72737954244304	37.0	37.0	37.0	27.0	37.0
18	34.680920273455364	37.0	37.0	37.0	27.0	37.0
19	34.665357239179635	37.0	37.0	37.0	27.0	37.0
20	34.68151127475697	37.0	37.0	37.0	27.0	37.0
21	34.672444564312435	37.0	37.0	37.0	27.0	37.0
22	34.57205877179611	37.0	37.0	37.0	27.0	37.0
23	34.616132928388	37.0	37.0	37.0	27.0	37.0
24	34.59337703303275	37.0	37.0	37.0	27.0	37.0
25	34.60945133033924	37.0	37.0	37.0	27.0	37.0
26	34.46494916685233	37.0	37.0	37.0	27.0	37.0
27	34.48889410054058	37.0	37.0	37.0	27.0	37.0
28	34.508054737977695	37.0	37.0	37.0	27.0	37.0
29	34.52140855310217	37.0	37.0	37.0	27.0	37.0
30	34.531758111610124	37.0	37.0	37.0	27.0	37.0
31	34.53010471511158	37.0	37.0	37.0	27.0	37.0
32	34.49191946434644	37.0	37.0	37.0	27.0	37.0
33	34.48989317416949	37.0	37.0	37.0	27.0	37.0
34	34.45511556186165	37.0	37.0	37.0	27.0	37.0
35	34.4052650711195	37.0	37.0	37.0	27.0	37.0
36	34.42924518345665	37.0	37.0	37.0	27.0	37.0
37	34.453025950116675	37.0	37.0	37.0	27.0	37.0
38	34.42003775841649	37.0	37.0	37.0	27.0	37.0
39	34.322949377924225	37.0	37.0	37.0	27.0	37.0
40	34.12020778855287	37.0	37.0	37.0	27.0	37.0
41	34.308629322576486	37.0	37.0	37.0	27.0	37.0
42	34.340294797077824	37.0	37.0	37.0	27.0	37.0
43	33.903383013403065	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	9.0
16	18.0
17	29.0
18	31.0
19	41.0
20	135.0
21	307.0
22	728.0
23	1453.0
24	2537.0
25	4019.0
26	5846.0
27	7903.0
28	10542.0
29	13010.0
30	16297.0
31	20175.0
32	25003.0
33	32700.0
34	46288.0
35	83640.0
36	155684.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.918620058865606	21.980323409045603	14.000164167028226	24.100892365060567
2	15.984709013942473	22.889339696760047	38.17516621911608	22.950785070181404
3	17.863717914140643	27.77799927297459	30.981836090948534	23.376446721936233
4	13.286975691553607	19.123817117930557	38.659224428053804	28.929982762462036
5	13.840453100997902	38.08792316983079	35.60337245980839	12.468251269362915
6	28.81060988050986	40.21036832045404	17.950022866121788	13.028998932914316
7	25.04391468005019	33.285803069923425	24.2357438525311	17.43453839749528
8	23.557968550287878	37.10339004913285	21.086785726849516	18.251855673729757
9	24.91562987370865	16.32594190832444	20.394704440718115	38.363723777248794
10	14.180982422401764	29.085706914949753	34.80200283774435	21.931307824904138
11	32.663844557276704	24.520690908664502	24.833311835270113	17.98215269878868
12	21.4425591294457	26.589195464299536	30.993562307250322	20.974683099004444
13	26.699890946188393	22.64004033818408	27.585220276973228	23.0748484386543
14	21.15784659763834	21.955463830485815	28.036210555939917	28.850479015935925
15	23.11471757408037	29.766061984779373	24.94799423070158	22.171226210438675
16	22.39941837967143	29.61901523235498	25.301891438689477	22.679674949284117
17	21.829524267404636	28.80380867505482	26.766026806130466	22.600640251410077
18	21.424735280666987	28.59906893842564	28.26276105489042	21.713434726016956
19	23.556092355679592	27.590848860798083	28.520503289203674	20.332555494318648
20	23.28615485641248	27.187232495690616	28.492125845753353	21.03448680214355
21	21.700301363758957	28.51182588914035	27.654404953153765	22.133467793946927
22	21.55724152487717	28.07537611838788	27.85140538702377	22.515976969711183
23	21.66864057974413	28.065995145346452	28.19803234090456	22.067331934004855
24	22.654580846398293	28.405117320794098	27.228039728420832	21.712262104386777
25	22.5732009052639	27.505012957469017	28.147844135132914	21.773942002134174
26	22.275355011198535	27.80027908394798	27.76533495936866	22.15903094548482
27	22.14753925350907	27.598588163557263	27.55191782267616	22.701954760257507
28	21.44162103214156	28.029878399136948	28.46796984017167	22.060530728549818
29	22.73689888483683	28.069043961584917	27.415190140597335	21.77886701298092
30	21.945848333118352	28.381664888190528	27.71327055898873	21.959216219702387
31	22.388864784999825	27.830063673354523	27.97171636628009	21.809355175365564
32	21.50728784343156	27.99939023675231	27.85257800865395	22.640743911162186
33	21.606726157670707	27.690990748015338	28.399254212643203	22.30302888167075
34	22.07577480974214	27.626965607007588	27.909567419880627	22.387692163369646
35	22.243459702857677	28.226409784354882	27.534563022549513	21.995567490237924
36	22.513162677798753	28.0774868373222	27.275413642280043	22.133936842599
37	22.9172480915583	27.065514370478077	27.566927379542445	22.450310158421182
38	22.71461907386344	27.957879431043985	27.471006930193838	21.856494564898743
39	22.05888905826757	27.678560958735442	27.969371123019737	22.29317885997725
40	22.12221062629721	27.696150283188125	28.109851194315134	22.07178789619953
41	21.731727623447743	27.785504051407734	28.148078659458953	22.334689665685573
42	22.187642913261175	27.795823121753305	27.654639477479805	22.361894487505715
43	22.18482862134875	27.450368789502694	27.823731516551554	22.541071072597006
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	16.0
2	16.0
3	75.5
4	135.0
5	135.0
6	172.5
7	210.0
8	194.5
9	179.0
10	285.5
11	392.0
12	392.0
13	614.5
14	837.0
15	1676.5
16	2516.0
17	2854.0
18	3192.0
19	3192.0
20	3408.5
21	3625.0
22	4446.5
23	5268.0
24	6465.5
25	7663.0
26	7663.0
27	9351.5
28	11040.0
29	13883.0
30	16726.0
31	18163.5
32	19601.0
33	19601.0
34	22106.5
35	24612.0
36	26001.5
37	27391.0
38	29970.5
39	32550.0
40	32550.0
41	34518.0
42	36486.0
43	34441.0
44	32396.0
45	32346.0
46	32296.0
47	32296.0
48	31591.5
49	30887.0
50	30951.5
51	31016.0
52	30562.5
53	30109.0
54	30109.0
55	25822.5
56	21536.0
57	18878.0
58	16220.0
59	14304.0
60	12388.0
61	12388.0
62	10902.5
63	9417.0
64	7857.5
65	6298.0
66	5263.5
67	4229.0
68	4229.0
69	3487.0
70	2745.0
71	2269.0
72	1793.0
73	1468.5
74	1144.0
75	1144.0
76	876.5
77	609.0
78	484.0
79	359.0
80	276.0
81	193.0
82	193.0
83	170.0
84	147.0
85	117.0
86	87.0
87	68.0
88	49.0
89	49.0
90	33.0
91	17.0
92	10.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	426395.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.83095230539295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.213483390932	54.269411531517505
2	5.992926227112106	7.291108189823901
3	1.5445516611513026	2.8186964529813103
4	0.7659350500446984	1.8637023399319126
5	0.44291293928459113	1.3471407942531184
6	0.30811492986196404	1.1245754761807578
7	0.2110415113076453	0.8986499276169393
8	0.1404106687987728	0.6833051757493178
9	0.1284807988781766	0.703404841384543
>10	1.0194613657547935	12.69432700520598
>50	0.14664797702263987	6.20621557349062
>100	0.0821228671308332	8.438064801392299
>500	0.00351955144846428	1.356903431736959
>1k	3.9106127205158666E-4	0.30449445873480147
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1280	0.3001911373257191	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	964	0.2260814502984322	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	705	0.16533964985518124	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	656	0.15384795787943104	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	634	0.14868842270664523	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	620	0.1454050821421452	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	555	0.13016100094982352	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	547	0.12828480634153777	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	512	0.12007645493028764	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	511	0.11984193060425193	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	500	0.11726216301785902	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	481	0.1128062008231804	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	479	0.11233715217110894	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	465	0.10905381160660889	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	435	0.10201808182553734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.690486520714361E-4	0.0
4	0.0	0.0	2.3452432603571804E-4	4.690486520714361E-4	0.0
5	0.0	0.0	2.3452432603571804E-4	4.690486520714361E-4	0.0
6	0.0	0.0	4.690486520714361E-4	4.690486520714361E-4	0.0
7	0.0	0.0	0.0014071459562143083	7.035729781071542E-4	0.0
8	0.0	0.0	0.0016416702822500263	7.035729781071542E-4	0.0
9	0.0	0.0	0.002579767586392899	0.0014071459562143083	0.0
10	0.0	0.0	0.003048816238464335	0.0014071459562143083	0.0
11	0.0	0.0	0.005394059498821515	0.0016416702822500263	0.0
12	0.0	0.0	0.0058631081508929515	0.0016416702822500263	0.0
13	0.0	0.0	0.0058631081508929515	0.0016416702822500263	0.0
14	0.0	0.0	0.0058631081508929515	0.0016416702822500263	0.0
15	0.0	0.0	0.007035729781071542	0.0016416702822500263	0.0
16	0.0	0.0	0.007504778433142977	0.0016416702822500263	0.0
17	0.0	0.0	0.007504778433142977	0.0016416702822500263	0.0
18	0.0	0.0	0.007973827085214415	0.0018761946082857443	0.0
19	0.0	0.0	0.007973827085214415	0.0021107189343214624	0.0
20	0.0	0.0	0.008208351411250132	0.0023452432603571806	0.0
21	0.0	0.0	0.008677400063321569	0.0023452432603571806	0.0
22	0.0	0.0	0.009380973041428722	0.0023452432603571806	0.0
23	0.0	0.0	0.009380973041428722	0.0023452432603571806	0.0
24	0.0	0.0	0.00961549736746444	0.002579767586392899	0.0
25	0.0	0.0	0.00961549736746444	0.002579767586392899	0.0
26	0.0	0.0	0.00961549736746444	0.002579767586392899	0.0
27	0.0	0.0	0.00961549736746444	0.003517864890535771	0.0
28	0.0	0.0	0.00961549736746444	0.011491691975750184	0.0
29	0.0	0.0	0.00961549736746444	0.02439052990771468	0.0
30	2.3452432603571804E-4	0.0	0.00961549736746444	0.039634611100036354	0.0
31	2.3452432603571804E-4	0.0	0.00961549736746444	0.09005734119771573	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGA	30	3.595714E-4	30.833334	26
CGTCAAA	50	9.073585E-6	25.900002	27
GTATCAA	670	0.0	24.850746	1
GGTATCA	230	0.0	24.130436	1
ACGCCGG	40	0.0019292061	23.125	14
GATAGCG	40	0.0019292061	23.125	25
AGCTATA	50	2.6982406E-4	22.2	2
TCGCCTA	60	3.7192123E-5	21.583334	35
CGGCGTC	60	3.7192123E-5	21.583334	24
CCGGCGT	60	3.7192123E-5	21.583334	23
TTGCGCC	45	0.0038219206	20.555555	13
CGGTGGG	45	0.0038219206	20.555555	18
TTTGACG	45	0.0038219206	20.555555	10
TCGCCAT	100	1.2840246E-8	20.35	13
ACACGAT	55	5.135803E-4	20.181818	15
CTCTAAC	55	5.135803E-4	20.181818	1
GCGTCAA	65	6.88912E-5	19.923077	26
CCCACCG	75	9.243373E-6	19.733334	19
CTAGCAC	105	2.2493623E-8	19.38095	3
GTCGCCA	110	3.8346116E-8	18.5	12
>>END_MODULE