FastQCFastQC Report
Fri 10 Feb 2017
ERR1633369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644288
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57480.892147611006258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42690.6625918843746896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24400.37871262540975464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10850.16840295023343596No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7000.10864706466673288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT303.5977375E-430.8333347
GGTATCA8550.025.9649121
TAAACCG551.9009763E-523.5454545
GTATCAA22450.022.9910931
AGGACCG652.679808E-622.769235
CGAGTCG1750.022.221
GGACCGT855.1717507E-821.7647066
CCGCCGT603.7230137E-521.58333424
TAGGACC705.0961808E-621.1428594
GCCGCTC1800.020.55555727
ATTGCCG450.003824009620.55555711
CCGTTTA450.003824009620.55555727
CGGCCTT555.139722E-420.18181824
GGCGAGT1950.019.92307519
GCTTCGC2350.019.68085122
GTCAACC851.2435703E-619.58823615
ATACGGA851.2435703E-619.5882364
CGCTCTC1800.019.52777929
CCGCTCT1900.019.47368428
GCGAGTC2000.019.42520
Produced by FastQC (version 0.11.5)