##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633369.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644288
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.130961619648357	33.0	33.0	33.0	27.0	33.0
2	31.447285996324624	33.0	33.0	33.0	27.0	33.0
3	31.202230989867886	33.0	33.0	33.0	27.0	33.0
4	31.51996312208205	33.0	33.0	33.0	27.0	33.0
5	31.647444931459223	33.0	33.0	33.0	27.0	33.0
6	34.46082497268302	37.0	37.0	37.0	27.0	37.0
7	34.62940175821993	37.0	37.0	37.0	27.0	37.0
8	34.775510330783746	37.0	37.0	37.0	27.0	37.0
9	34.903563313300886	37.0	37.0	37.0	27.0	37.0
10	34.92564505314394	37.0	37.0	37.0	27.0	37.0
11	34.924625322836995	37.0	37.0	37.0	27.0	37.0
12	34.9237266564021	37.0	37.0	37.0	27.0	37.0
13	34.944512391973774	37.0	37.0	37.0	27.0	37.0
14	34.923054596702094	37.0	37.0	37.0	27.0	37.0
15	34.94162238005364	37.0	37.0	37.0	27.0	37.0
16	34.914117599582795	37.0	37.0	37.0	27.0	37.0
17	34.92367698917254	37.0	37.0	37.0	27.0	37.0
18	34.89620790702295	37.0	37.0	37.0	27.0	37.0
19	34.88410772822092	37.0	37.0	37.0	27.0	37.0
20	34.89973117612	37.0	37.0	37.0	27.0	37.0
21	34.89161213618755	37.0	37.0	37.0	27.0	37.0
22	34.7761653173736	37.0	37.0	37.0	27.0	37.0
23	34.81749776497467	37.0	37.0	37.0	27.0	37.0
24	34.815234801827756	37.0	37.0	37.0	27.0	37.0
25	34.823696856064366	37.0	37.0	37.0	27.0	37.0
26	34.695392433197576	37.0	37.0	37.0	27.0	37.0
27	34.71238017780868	37.0	37.0	37.0	27.0	37.0
28	34.728284555974966	37.0	37.0	37.0	27.0	37.0
29	34.7312459645376	37.0	37.0	37.0	27.0	37.0
30	34.72232448842754	37.0	37.0	37.0	27.0	37.0
31	34.71057353233336	37.0	37.0	37.0	27.0	37.0
32	34.69396915665044	37.0	37.0	37.0	27.0	37.0
33	34.65710520760902	37.0	37.0	37.0	27.0	37.0
34	34.62170023343598	37.0	37.0	37.0	27.0	37.0
35	34.55814014850502	37.0	37.0	37.0	27.0	37.0
36	34.57705870666534	37.0	37.0	37.0	27.0	37.0
37	34.570131680242376	37.0	37.0	37.0	27.0	37.0
38	34.5453430763882	37.0	37.0	37.0	27.0	37.0
39	34.418117363663455	37.0	37.0	37.0	27.0	37.0
40	34.1781718734479	37.0	37.0	37.0	27.0	37.0
41	34.34745641700606	37.0	37.0	37.0	27.0	37.0
42	34.365355244859444	37.0	37.0	37.0	27.0	37.0
43	33.94689176268998	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	25.0
16	37.0
17	42.0
18	41.0
19	66.0
20	135.0
21	388.0
22	909.0
23	1784.0
24	3245.0
25	5274.0
26	7741.0
27	10936.0
28	14678.0
29	18576.0
30	23543.0
31	29144.0
32	36066.0
33	46460.0
34	65307.0
35	118745.0
36	261142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.096683470746	21.286598539783448	14.545203387305056	23.07151460216549
2	15.46482318466276	23.463264875335256	39.04992798251713	22.02198395748485
3	17.705280868183173	28.640452716797455	31.36423462799245	22.29003178702692
4	12.288138223899871	19.070819260951623	40.263360484752155	28.377682030396343
5	13.505606188536804	37.65815908413629	35.04969827158041	13.786536455746498
6	27.098285238899372	41.72186972285686	17.61913926691169	13.560705771332074
7	24.433172742624418	34.04176393165789	23.797587414324028	17.72747591139366
8	24.064238353034668	35.76257822588656	21.202629879805304	18.970553541273468
9	25.999552994933943	15.38597645773319	20.82950481772127	37.7849657296116
10	14.413119598688784	28.797059700009935	34.18626452766465	22.603556173636637
11	32.39886510380451	24.919290751961857	24.705411244660773	17.97643289957286
12	22.192404638919243	26.96728792092977	31.123193354524687	19.717114085626303
13	28.058725290553294	23.29237856362372	27.117686500447004	21.53120964537598
14	21.762783103208502	22.23788119598689	29.570471590344688	26.42886411045992
15	23.227966375285586	29.198588208999706	26.106337538492102	21.467107877222606
16	21.082497268302376	29.729872355220028	26.480083440945663	22.707546935531937
17	21.318727028906327	28.12717294129333	27.411343995231945	23.142756034568393
18	22.42351246647462	26.85413976358399	29.371181831727426	21.351165938213967
19	23.988030197675574	26.639949836098143	29.879184464090592	19.49283550213569
20	23.9354139763584	26.125894010132118	29.419762590642694	20.51892942286679
21	21.94158512963147	27.54637677560346	29.176703585973975	21.3353345087911
22	21.729412933346577	27.37719777490811	29.40858746399126	21.484801827754048
23	21.448793086321643	27.195912387007056	29.96423959471541	21.391054931955896
24	21.708459570875135	27.27320701301281	29.598098986788518	21.420234429323532
25	21.78466772623423	27.147486838184165	30.040292539982122	21.027552895599484
26	21.91194000198669	27.40622206218337	29.28690275156452	21.39493518426542
27	21.92978916261051	27.31340642693951	29.23102711830734	21.525777292142646
28	21.26548996721963	27.421587861329094	29.631313946558063	21.681608224893214
29	21.942671600278135	27.486310469851993	29.123311314194893	21.447706615674978
30	22.037504966722956	27.52759635442535	29.452356710042714	20.98254196880898
31	21.928547481871462	27.331255587563323	29.433731498956988	21.306465431608224
32	20.97819608622231	27.284847769941393	29.66514974669713	22.071806397139166
33	21.12223105195192	27.296022896592824	30.0404477500745	21.54129830138075
34	21.3713432502235	27.07640061587365	29.48650293036654	22.065753203536307
35	21.36218585477302	27.782916956392175	29.5212699910599	21.333627197774906
36	21.672606039535115	27.46970298996722	29.31515098837787	21.542539982119795
37	22.003048326214365	27.1938946558061	29.187723502533032	21.61533351544651
38	21.81679621535711	27.558172742624414	29.063865848812952	21.56116519320552
39	21.532140905930266	27.301455249826162	29.135572911492996	22.030830932750572
40	21.515223005860733	27.588128290453955	29.496436376278933	21.400212327406376
41	21.592672841958876	27.199482219131816	29.35410872156551	21.853736217343798
42	21.315622827058707	27.54249652329393	29.326015694844543	21.815864954802823
43	21.359702493294925	27.3723862620443	29.221404092579718	22.04650715208106
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	66.0
2	90.0
3	300.5
4	511.0
5	511.0
6	657.0
7	803.0
8	792.5
9	782.0
10	1106.5
11	1431.0
12	1431.0
13	2312.0
14	3193.0
15	5744.5
16	8296.0
17	9301.5
18	10307.0
19	10307.0
20	9721.0
21	9135.0
22	9717.0
23	10299.0
24	12477.0
25	14655.0
26	14655.0
27	17112.0
28	19569.0
29	23178.5
30	26788.0
31	28822.5
32	30857.0
33	30857.0
34	33471.5
35	36086.0
36	37517.5
37	38949.0
38	40608.0
39	42267.0
40	42267.0
41	43423.5
42	44580.0
43	43999.5
44	43419.0
45	44069.5
46	44720.0
47	44720.0
48	43748.0
49	42776.0
50	43671.5
51	44567.0
52	44057.5
53	43548.0
54	43548.0
55	38803.5
56	34059.0
57	30529.0
58	26999.0
59	24132.0
60	21265.0
61	21265.0
62	18397.0
63	15529.0
64	12529.0
65	9529.0
66	8314.5
67	7100.0
68	7100.0
69	5840.0
70	4580.0
71	3879.5
72	3179.0
73	2640.0
74	2101.0
75	2101.0
76	1495.5
77	890.0
78	705.0
79	520.0
80	405.0
81	290.0
82	290.0
83	250.0
84	210.0
85	188.5
86	167.0
87	146.0
88	125.0
89	125.0
90	90.0
91	55.0
92	37.0
93	19.0
94	10.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	644288.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.26671649284505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.43023835299637	57.473877741195764
2	6.245382419693443	8.027404427116743
3	1.4336297879972766	2.7640403742275557
4	0.6756793894300555	1.7369478304224009
5	0.40004127110098897	1.2854669477642315
6	0.26541023121826807	1.023422645040293
7	0.19436125095243312	0.8743671588508312
8	0.14853451236835027	0.7636660316623803
9	0.12581662929093967	0.7277239480253586
>10	0.9185255735376626	12.033854298920362
>50	0.10548636681637608	4.630768916814684
>100	0.05445240281252305	5.977044173818009
>500	0.0014650870711863033	0.556307015232128
>1k	7.325435355931516E-4	1.2230907973820044
>5k	2.4418117853105056E-4	0.9020176935272982
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5748	0.892147611006258	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4269	0.6625918843746896	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2440	0.37871262540975464	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1085	0.16840295023343596	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	700	0.10864706466673288	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.65630277143141E-4	0.0	0.0
2	0.0	0.0	4.65630277143141E-4	1.5521009238104699E-4	0.0
3	0.0	0.0	6.208403695241879E-4	3.1042018476209397E-4	0.0
4	0.0	0.0	7.760504619052349E-4	6.208403695241879E-4	0.0
5	0.0	0.0	0.0017073110161915169	6.208403695241879E-4	0.0
6	0.0	0.0	0.0024833614780967518	7.760504619052349E-4	0.0
7	0.0	0.0	0.0068292440647660674	7.760504619052349E-4	0.0
8	0.0	0.0	0.00822613489619549	7.760504619052349E-4	0.0
9	0.0	0.0	0.009157395450481772	0.002017731200953611	0.0
10	3.1042018476209397E-4	0.0	0.010709496374292242	0.0021729412933346576	0.0
11	3.1042018476209397E-4	0.0	0.017228320254296214	0.0023281513857157047	0.0
12	3.1042018476209397E-4	0.0	0.018314790900963544	0.0023281513857157047	0.0
13	3.1042018476209397E-4	0.0	0.019401261547630874	0.002638571570477799	0.0
14	3.1042018476209397E-4	0.0	0.02064294228667925	0.002638571570477799	0.0
15	4.65630277143141E-4	0.0	0.02359193404191914	0.002638571570477799	0.0
16	4.65630277143141E-4	0.0	0.024523194596205423	0.002638571570477799	0.0
17	4.65630277143141E-4	0.0	0.02529924505811066	0.002793781662858846	0.0
18	4.65630277143141E-4	0.0	0.025920085427634847	0.0029489917552398925	0.0
19	4.65630277143141E-4	0.0	0.02623050561239694	0.0029489917552398925	0.0
20	4.65630277143141E-4	0.0	0.027161766166683223	0.0031042018476209396	0.0
21	4.65630277143141E-4	0.0	0.02778260653620741	0.0038802523095261745	0.0
22	4.65630277143141E-4	0.0	0.0284034469057316	0.004811512863812457	0.0
23	4.65630277143141E-4	0.0	0.028713867090493693	0.005277143140955598	0.0
24	4.65630277143141E-4	0.0	0.02886907718287474	0.006208403695241879	0.0
25	4.65630277143141E-4	0.0	0.02886907718287474	0.00667403397238502	0.0
26	4.65630277143141E-4	0.0	0.029024287275255786	0.007450084434290256	0.0
27	4.65630277143141E-4	0.0	0.029024287275255786	0.009002185358100726	0.0
28	4.65630277143141E-4	0.0	0.029024287275255786	0.01893563127048773	0.0
29	4.65630277143141E-4	0.0	0.029024287275255786	0.044700506605741534	0.0
30	4.65630277143141E-4	0.0	0.029024287275255786	0.07900193702195292	0.0
31	4.65630277143141E-4	0.0	0.029024287275255786	0.15086420979437767	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTT	30	3.5977375E-4	30.833334	7
GGTATCA	855	0.0	25.964912	1
TAAACCG	55	1.9009763E-5	23.545454	5
GTATCAA	2245	0.0	22.991093	1
AGGACCG	65	2.679808E-6	22.76923	5
CGAGTCG	175	0.0	22.2	21
GGACCGT	85	5.1717507E-8	21.764706	6
CCGCCGT	60	3.7230137E-5	21.583334	24
TAGGACC	70	5.0961808E-6	21.142859	4
GCCGCTC	180	0.0	20.555557	27
ATTGCCG	45	0.0038240096	20.555557	11
CCGTTTA	45	0.0038240096	20.555557	27
CGGCCTT	55	5.139722E-4	20.181818	24
GGCGAGT	195	0.0	19.923075	19
GCTTCGC	235	0.0	19.680851	22
GTCAACC	85	1.2435703E-6	19.588236	15
ATACGGA	85	1.2435703E-6	19.588236	4
CGCTCTC	180	0.0	19.527779	29
CCGCTCT	190	0.0	19.473684	28
GCGAGTC	200	0.0	19.425	20
>>END_MODULE