FastQCFastQC Report
Fri 10 Feb 2017
ERR1633368.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633368.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488028
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19380.39710836263493077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.2712959092511086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8560.17539977214422123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3650.027.8767131
GTATCAA9100.022.3626371
GCCGGCA753.7316386E-722.215
CTGACGC502.6990153E-422.227
TGACGCA502.6990153E-422.228
CCGCGTC450.003822700820.55555525
ACGAAAT450.003822700820.55555519
CGAACCG656.8917325E-519.92307731
GCGAACC656.8917325E-519.92307730
ATACCAT759.247864E-619.7333346
TCTGACG609.2264335E-418.526
GTCGCCC902.147055E-618.537
CGAACTA1306.9303496E-1018.524
GCTTCGC1002.866509E-718.522
TGCTCGC1205.151378E-918.510
ACCGTGG801.613566E-518.58
GACGCAG609.2264335E-418.529
CGGTACC500.00702922318.514
GGCGAAC701.2173741E-418.529
CGCCGCT1002.866509E-718.526
Produced by FastQC (version 0.11.5)