##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633368.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	488028
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.020779545435918	33.0	33.0	33.0	27.0	33.0
2	31.357614727023858	33.0	33.0	33.0	27.0	33.0
3	31.104438679747883	33.0	33.0	33.0	27.0	33.0
4	31.421967592023407	33.0	33.0	33.0	27.0	33.0
5	31.546163334890622	33.0	33.0	33.0	27.0	33.0
6	34.12799265615907	37.0	33.0	37.0	27.0	37.0
7	34.30757661445655	37.0	33.0	37.0	27.0	37.0
8	34.40815281090429	37.0	37.0	37.0	27.0	37.0
9	34.51567533010401	37.0	37.0	37.0	27.0	37.0
10	34.6038874818658	37.0	37.0	37.0	27.0	37.0
11	34.59535723360135	37.0	37.0	37.0	27.0	37.0
12	34.584677518503035	37.0	37.0	37.0	27.0	37.0
13	34.64950166793709	37.0	37.0	37.0	27.0	37.0
14	34.617536288901455	37.0	37.0	37.0	27.0	37.0
15	34.6348570164007	37.0	37.0	37.0	27.0	37.0
16	34.61343611432131	37.0	37.0	37.0	27.0	37.0
17	34.6323715032744	37.0	37.0	37.0	27.0	37.0
18	34.59266066701091	37.0	37.0	37.0	27.0	37.0
19	34.57572721237306	37.0	37.0	37.0	27.0	37.0
20	34.58591105428377	37.0	37.0	37.0	27.0	37.0
21	34.595539600186875	37.0	37.0	37.0	27.0	37.0
22	34.49276885752457	37.0	37.0	37.0	27.0	37.0
23	34.53447548091503	37.0	37.0	37.0	27.0	37.0
24	34.53163343086872	37.0	37.0	37.0	27.0	37.0
25	34.515829009810915	37.0	37.0	37.0	27.0	37.0
26	34.38804945617874	37.0	37.0	37.0	27.0	37.0
27	34.39130746596507	37.0	37.0	37.0	27.0	37.0
28	34.41022031522782	37.0	37.0	37.0	27.0	37.0
29	34.416008917521125	37.0	37.0	37.0	27.0	37.0
30	34.422127418918585	37.0	37.0	37.0	27.0	37.0
31	34.42001073708886	37.0	37.0	37.0	27.0	37.0
32	34.38436114321309	37.0	37.0	37.0	27.0	37.0
33	34.38407017630136	37.0	37.0	37.0	27.0	37.0
34	34.3526170629554	37.0	37.0	37.0	27.0	37.0
35	34.302451908497055	37.0	37.0	37.0	27.0	37.0
36	34.335349611087885	37.0	37.0	37.0	27.0	37.0
37	34.33956658224528	37.0	37.0	37.0	27.0	37.0
38	34.32609809273239	37.0	37.0	37.0	27.0	37.0
39	34.22665297892744	37.0	37.0	37.0	27.0	37.0
40	33.99557197537846	37.0	33.0	37.0	27.0	37.0
41	34.19434335734835	37.0	37.0	37.0	27.0	37.0
42	34.23664830706435	37.0	37.0	37.0	27.0	37.0
43	33.78988295753523	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	18.0
16	27.0
17	20.0
18	34.0
19	50.0
20	173.0
21	364.0
22	852.0
23	1798.0
24	3030.0
25	4781.0
26	6980.0
27	9420.0
28	12457.0
29	15835.0
30	19724.0
31	24037.0
32	29981.0
33	38181.0
34	54705.0
35	95915.0
36	169644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.22248723433901	21.86001622857705	13.839779684772186	24.077716852311752
2	16.18718598113223	22.64132385846714	37.45215438458449	23.71933577581614
3	18.034825870646767	27.812953355135363	29.95360921914316	24.19861155507471
4	13.162974255575499	19.45093314318031	38.73486767152705	28.651224929717145
5	14.381346971895056	37.352365028236086	35.13958215512225	13.126705844746613
6	29.115747457113116	40.21142229544207	17.76598883670609	12.906841410738728
7	26.12555017335071	32.65714262296426	23.75867778078307	17.458629422901964
8	24.287950691353775	36.3352102748203	20.500463088183466	18.87637594564246
9	25.34793085642627	15.859540846017032	20.540419811978	38.252108485578695
10	14.574163777488177	28.64979058578606	34.129394215086016	22.646651421639742
11	33.30259739195292	23.8006835673363	24.75370265640496	18.143016384305817
12	22.41818092404534	25.9507651200341	30.88388371159032	20.747170244330242
13	27.42199218077651	22.706484054193613	26.562205447228436	23.309318317801438
14	21.74752268312474	21.4469251764243	27.894301146655522	28.91125099379544
15	23.347840697664886	29.54338685485259	24.39450195480587	22.71427049267665
16	22.529854844394173	29.141360741596795	25.496487906431597	22.832296507577436
17	22.069430442515596	28.071954887834305	26.85952445351496	22.99909021613514
18	22.474735056185303	27.840820608653598	28.218053062529197	21.4663912726319
19	24.097182948519347	27.011360003934197	27.730990844787595	21.160466202758858
20	23.506438155187816	27.108895391248044	27.815207324169926	21.569459129394215
21	22.714065586400782	27.319948855393545	27.897374740793563	22.068610817412114
22	22.336013507421708	27.21442212332079	27.784471382789512	22.66509298646799
23	22.417566205217735	27.27077134918488	28.159859680182286	22.1518027654151
24	22.577393100395877	27.76664453678887	26.88103961248125	22.774922750334
25	22.681485488537543	27.287778570082043	28.057816354799314	21.972919586581096
26	22.785782782955078	27.342898358290917	27.933438245346576	21.937880613407426
27	22.5987033530863	27.29003253911661	27.542067258435992	22.569196849361102
28	22.196062521002894	27.400681928086097	27.931594088863754	22.47166146204726
29	23.299277910283838	27.110534641454997	27.56317260485054	22.027014843410626
30	23.049497159999017	26.908292147171885	27.874835050447928	22.167375642381174
31	22.841517289991558	27.100494233937393	27.71521306154565	22.342775414525395
32	22.04156318899735	27.231839156769695	27.848402140860774	22.878195513372184
33	21.915750735613532	27.456621341398446	28.197972247493997	22.42965567549403
34	22.642143483570614	26.909931397378838	27.949420934864396	22.498504184186153
35	22.458957272943355	27.529363069332085	27.706402091683263	22.305277566041294
36	23.28534428352472	27.151106084077142	27.400477021810225	22.163072610587918
37	22.7206225872286	27.041686132762877	27.6023097035416	22.635381576466923
38	22.75197324743662	27.310318260427678	27.27855778766792	22.659150704467777
39	22.52001934315244	27.1131984230413	27.767054349340615	22.599727884465644
40	22.55997606694698	27.5822288885064	27.75045694099519	22.107338103551434
41	22.34912750907735	26.834116075307158	27.799839353479715	23.016917062135782
42	22.20712745989984	27.58058963829944	27.741441064856936	22.47084183694378
43	22.657101641709083	26.887391707033203	27.77340644389256	22.682100207365153
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	15.0
2	21.0
3	96.5
4	172.0
5	172.0
6	213.0
7	254.0
8	264.0
9	274.0
10	349.0
11	424.0
12	424.0
13	719.5
14	1015.0
15	1817.0
16	2619.0
17	3138.0
18	3657.0
19	3657.0
20	3669.5
21	3682.0
22	4177.5
23	4673.0
24	6064.0
25	7455.0
26	7455.0
27	9425.5
28	11396.0
29	13725.5
30	16055.0
31	18445.5
32	20836.0
33	20836.0
34	23921.5
35	27007.0
36	29169.0
37	31331.0
38	33474.5
39	35618.0
40	35618.0
41	37382.0
42	39146.0
43	40005.0
44	40864.0
45	39773.5
46	38683.0
47	38683.0
48	37032.5
49	35382.0
50	35150.5
51	34919.0
52	33469.0
53	32019.0
54	32019.0
55	29741.0
56	27463.0
57	23747.5
58	20032.0
59	18660.5
60	17289.0
61	17289.0
62	15118.0
63	12947.0
64	10714.0
65	8481.0
66	7029.0
67	5577.0
68	5577.0
69	4604.0
70	3631.0
71	2867.0
72	2103.0
73	1699.0
74	1295.0
75	1295.0
76	1008.0
77	721.0
78	542.5
79	364.0
80	303.5
81	243.0
82	243.0
83	204.5
84	166.0
85	139.0
86	112.0
87	83.0
88	54.0
89	54.0
90	41.5
91	29.0
92	18.5
93	8.0
94	4.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	488028.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.87399493460706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.45901483059951	55.86356670275436
2	6.772155637918919	8.651292698256073
3	1.9738384227024948	3.782308362402958
4	0.9379180053113649	2.3963427968133955
5	0.5831049673411453	1.8622621865146263
6	0.36898489683650315	1.4141123658888783
7	0.28035670529587503	1.253525194176629
8	0.19401645582685	0.9914084893371705
9	0.16837628971582164	0.9679379648774689
>10	1.1323076688311489	13.816463461869763
>50	0.09257236041463328	4.030568408171469
>100	0.03637931331343665	4.1158395866161595
>500	3.2481529744139866E-4	0.17759646567917292
>1k	6.496305948827973E-4	0.6767753166418949
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1938	0.39710836263493077	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1324	0.2712959092511086	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	856	0.17539977214422123	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.0490627586941732E-4	0.0	0.0
2	0.0	0.0	2.0490627586941732E-4	0.0	0.0
3	0.0	0.0	4.0981255173883465E-4	0.0	0.0
4	0.0	0.0	4.0981255173883465E-4	2.0490627586941732E-4	0.0
5	0.0	0.0	6.14718827608252E-4	2.0490627586941732E-4	0.0
6	0.0	0.0	6.14718827608252E-4	2.0490627586941732E-4	0.0
7	0.0	0.0	0.0020490627586941734	2.0490627586941732E-4	0.0
8	0.0	0.0	0.002458875310433008	2.0490627586941732E-4	0.0
9	0.0	0.0	0.003278500413910677	8.196251034776693E-4	0.0
10	0.0	0.0	0.0038932192415189293	0.0010245313793470867	0.0
11	0.0	0.0	0.005737375724343685	0.001229437655216504	0.0
12	0.0	0.0	0.005942282000213102	0.001229437655216504	0.0
13	0.0	0.0	0.00614718827608252	0.001229437655216504	0.0
14	0.0	0.0	0.006557000827821354	0.0014343439310859213	0.0
15	0.0	0.0	0.0073766259312990235	0.0014343439310859213	0.0
16	0.0	0.0	0.0077864384830378585	0.0014343439310859213	0.0
17	0.0	0.0	0.007991344758907276	0.0016392502069553386	0.0
18	0.0	0.0	0.007991344758907276	0.0018441564828247559	0.0
19	0.0	0.0	0.008196251034776694	0.0018441564828247559	0.0
20	0.0	0.0	0.00840115731064611	0.0018441564828247559	0.0
21	0.0	0.0	0.00840115731064611	0.0018441564828247559	0.0
22	0.0	0.0	0.008606063586515528	0.0022539690345635905	0.0
23	0.0	0.0	0.008606063586515528	0.002458875310433008	0.0
24	0.0	0.0	0.008606063586515528	0.0028686878621718426	0.0
25	0.0	0.0	0.008606063586515528	0.00307359413804126	0.0
26	0.0	0.0	0.008606063586515528	0.00307359413804126	0.0
27	0.0	0.0	0.008606063586515528	0.0034834066897800947	0.0
28	0.0	0.0	0.008606063586515528	0.0077864384830378585	0.0
29	0.0	0.0	0.008606063586515528	0.018646471104116977	0.0
30	0.0	0.0	0.008606063586515528	0.034629160621931526	0.0
31	0.0	0.0	0.008606063586515528	0.06946322751973247	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	365	0.0	27.876713	1
GTATCAA	910	0.0	22.362637	1
GCCGGCA	75	3.7316386E-7	22.2	15
CTGACGC	50	2.6990153E-4	22.2	27
TGACGCA	50	2.6990153E-4	22.2	28
CCGCGTC	45	0.0038227008	20.555555	25
ACGAAAT	45	0.0038227008	20.555555	19
CGAACCG	65	6.8917325E-5	19.923077	31
GCGAACC	65	6.8917325E-5	19.923077	30
ATACCAT	75	9.247864E-6	19.733334	6
TCTGACG	60	9.2264335E-4	18.5	26
GTCGCCC	90	2.147055E-6	18.5	37
CGAACTA	130	6.9303496E-10	18.5	24
GCTTCGC	100	2.866509E-7	18.5	22
TGCTCGC	120	5.151378E-9	18.5	10
ACCGTGG	80	1.613566E-5	18.5	8
GACGCAG	60	9.2264335E-4	18.5	29
CGGTACC	50	0.007029223	18.5	14
GGCGAAC	70	1.2173741E-4	18.5	29
CGCCGCT	100	2.866509E-7	18.5	26
>>END_MODULE