FastQCFastQC Report
Fri 10 Feb 2017
ERR1633367.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633367.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678519
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50300.7413204346525301No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35950.5298304100548401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19770.2913698805781415No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.13293658688997656No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6900.1016920675765896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22400.025.1897341
GGTATCA6700.024.8507461
TAATACG451.3224315E-424.6666682
GCCGGCA1500.024.66666615
CGGTTAT551.9011699E-523.54545424
CCGGTTA551.9011699E-523.54545423
GCCGCTC1700.022.8529427
CCGCTCT1750.021.14285728
GCTTCGC1750.021.14285722
CGGGCTA450.003824216320.55555717
TTCGCCG1800.020.55555724
CTCTAAC450.003824216320.5555571
CGAACCG1900.020.44736931
TAGGACC1550.020.2903214
CGCCGCT2100.020.26190626
CGCTCTC1750.020.08571429
GGCGAAC1950.019.92307729
CGAGTCG1500.019.73333421
GTCCGGC1600.019.6562515
GCGAACC2000.019.42500130
Produced by FastQC (version 0.11.5)