FastQCFastQC Report
Fri 10 Feb 2017
ERR1633366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841043
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT138011.6409386916007862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT100741.197798447879597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54750.650977417325868No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25280.30057916182644645No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG22410.266454866160232No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA20670.24576626878768387No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC20210.2402968694822976No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC20020.23803776976920327No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG19020.22614777127923305No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA18300.2175869723664545No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17610.20938287340837505No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT16880.20070317451069686No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC15980.19000217586972365No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT15420.18334377671534036No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC15360.18263037680594216No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG15210.18084687703244662No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14960.17787437740995407No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14810.17609087763645853No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG14740.17525857774216064No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG14540.1728805780441666No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG14330.17038367836127286No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14180.16860017858777732No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG14090.16753007872368No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT13750.16348747923709014No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG13570.16134727950889552No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA13480.16027717964479818No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13300.15813697991660355No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC13060.1552833802790107No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG12440.14791158121522918No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA12340.14672258136623217No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT12120.14410678169843874No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC12050.14327448180414082No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11800.14030198218164827No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC11680.13887518236285185No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG11410.1356648827705599No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT11350.13495148286116168No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC11200.13316798308766614No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT10910.1297198835255748No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10500.124844984144687No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA10450.1242504842201885No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG10110.12020788473359864No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA10070.11973228479399983No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC10020.11913778486950131No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT9970.11854328494500281No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT9890.11759208506580521No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9890.11759208506580521No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG9730.11568968530740996No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG9680.11509518538291146No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT9480.11271718568491743No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9440.1122415857453186No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA9440.1122415857453186No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT9400.1117659858057198No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG9360.111290385866121No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.10926908612282607No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9170.10903128615302665No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC9170.10903128615302665No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT9030.10736668636443082No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA8830.10498868666643679No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC8810.10475088669663739No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8750.10403748678723918No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC8750.10403748678723918No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC8640.10272958695334246No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA8460.10058938722514782No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC8430.10023268727044872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG352.387552E-531.7142852
AGACTAT250.005495514729.66
GTTCGCA250.005495514729.635
TTAAGGT250.005495514729.64
CTAGTGC701.9216532E-723.7857139
GGTATCA21700.023.44471
ACAACGC400.001930758823.1253
CGGCATT400.001930758823.12527
GTATCAA58000.022.8698271
CGTTATT652.681425E-622.7692310
TAATAGA502.7012665E-422.24
ACGCCCC502.7012665E-422.213
AGTGGTT502.7012665E-422.230
GCCTACC502.7012665E-422.28
TTCTCAA502.7012665E-422.232
CTTAGGA1350.021.9259262
AGTGACA4900.021.8979623
CACATGT4900.021.14285928
ACTAGTG1400.021.1428578
TTCTGTC705.0992385E-621.14285733
Produced by FastQC (version 0.11.5)