FastQCFastQC Report
Fri 10 Feb 2017
ERR1633365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648636
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT126391.9485504967346863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT97861.5087044197361847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54710.8434622808478098No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA43210.6661671569262267No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA31860.49118457809927296No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG30130.4645132246745478No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA26860.4140997416116281No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT23840.3675405003730906No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23610.36399459789465893No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC20130.31034355169925815No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT19960.3077226672586782No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC18420.2839805376204836No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC17920.2762720539717191No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17590.2711844547635346No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG16300.2512965669497222No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC15870.24466727101178473No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA15760.24297140460905653No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15750.24281723493608126No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC15030.23171701848186038No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC15000.23125450946293452No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA14960.23063783077103336No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14840.2287877946953299No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA14710.22678358894665115No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14520.2238543651601206No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG14130.2178417479140843No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13860.21367916674375148No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT13320.20535400440308588No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC13150.20273311996250593No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG13090.2018081019246542No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT12830.19779969042729664No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12820.19764552075432137No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG12750.19656633304349436No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC12600.19425378794886503No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC12160.18747032233795224No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC12080.18623696495414993No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11910.18361608051357No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11540.1779118026134843No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT11500.17729512392158314No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG11460.17667844522968199No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG11300.17421173046207736No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA11260.1735950517701762No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG11070.17066582798364568No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG11040.1702033189647198No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10660.1643448713916588No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA10570.16295734433488118No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG10570.16295734433488118No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG10430.16079896891322715No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG10420.16064479924025185No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG10340.15941144185644954No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA10310.15894893283752368No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG10220.15756140578074607No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10100.15571136970504257No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC9830.15154878853470977No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG9700.149544582786031No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9600.1480028860562781No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT9570.14754037703735223No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG9570.14754037703735223No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9460.14584451063462406No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC9460.14584451063462406No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT9390.144765322923797No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC9060.13967772371561243No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8980.13844436633181015No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT8960.13813602698585956No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC8890.13705683927503254No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA8790.13551514254527963No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC8580.13227757941279855No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT8330.12842333758841631No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8280.12765248922353986No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8190.12626496216676225No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC8180.12611079249378696No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT7960.12271905968833059No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7940.12241072034238No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA7940.12241072034238No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7930.12225655066940473No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA7900.12179404165047886No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG7870.121331532631553No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG7860.1211773629585777No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC7830.12071485393965183No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG7810.12040651459370125No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA7790.12009817524775065No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG7750.11948149655584951No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC7740.11932732688287422No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA7730.11917315720989892No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG7720.11901898753692364No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA7710.11886481786394834No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT7680.11840230884502248No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG7350.11331470963683792No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7240.11161884323410973No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA7120.10976880715840626No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG6920.10668541369890047No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG6750.10406452925832055No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC6710.10344785056641938No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6680.10298534154749352No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA6670.10283117187451822No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG6520.10051862677988886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCAA200.001841344637.012
AGGATAA303.5977643E-430.83333434
CGCGAGT250.00549459929.618
ACAACGC509.0831145E-625.9000023
TCGTTGC551.9010025E-523.54545416
CGCTCGT551.9010025E-523.54545413
GCTCGTT551.9010025E-523.54545414
GTTGCGC551.9010025E-523.5454549
TAACAAC400.001930284823.1250021
CTTATAC2100.022.90476437
TTGCGGG652.6798552E-622.7692319
AACAACG502.7003433E-422.22
GGTATCA25850.022.1856861
CTCGTTG603.7230642E-521.58333415
ACATTTC603.7230642E-521.58333436
AACATTT603.7230642E-521.58333435
TATACAC603.7230642E-521.58333437
GTATCAA59100.021.223351
GGGTCTC450.003824038420.55555518
CTAGTGC450.003824038420.5555559
Produced by FastQC (version 0.11.5)